##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546756_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2591706
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.42658426534491	31.0	31.0	33.0	30.0	34.0
2	31.82732454992966	31.0	31.0	34.0	30.0	34.0
3	31.88589870919001	31.0	31.0	34.0	30.0	34.0
4	35.633095729222376	37.0	35.0	37.0	33.0	37.0
5	35.538092283615505	37.0	35.0	37.0	33.0	37.0
6	35.5868721220694	37.0	35.0	37.0	33.0	37.0
7	36.00037812930942	37.0	35.0	37.0	35.0	37.0
8	22.02468489867292	35.0	0.0	37.0	0.0	37.0
9	29.8969007287092	37.0	18.0	39.0	17.0	39.0
10	34.2463635921667	35.0	32.0	39.0	27.0	39.0
11	35.912940356660826	37.0	35.0	39.0	32.0	39.0
12	36.50542152543537	38.0	35.0	39.0	32.0	39.0
13	36.705853210202086	39.0	35.0	39.0	33.0	39.0
14	37.89440121680468	40.0	37.0	41.0	33.0	41.0
15	38.10962624618688	40.0	37.0	41.0	33.0	41.0
16	38.117402977035205	40.0	37.0	41.0	33.0	41.0
17	38.07446600810432	40.0	37.0	41.0	33.0	41.0
18	37.947364785974955	39.0	37.0	41.0	33.0	41.0
19	37.89019202023686	39.0	37.0	41.0	33.0	41.0
20	37.696778106776	39.0	35.0	41.0	33.0	41.0
21	37.62046042259423	39.0	35.0	41.0	33.0	41.0
22	37.59101726816236	39.0	35.0	41.0	33.0	41.0
23	37.39446796820318	39.0	35.0	41.0	33.0	41.0
24	37.1714021574978	39.0	35.0	41.0	32.0	41.0
25	37.0325878012398	39.0	35.0	41.0	32.0	41.0
26	36.97541426380924	39.0	35.0	41.0	32.0	41.0
27	36.850974994848954	39.0	35.0	41.0	32.0	41.0
28	36.82290583885672	39.0	35.0	40.0	32.0	41.0
29	36.70856648092029	38.0	35.0	40.0	31.0	41.0
30	36.540512697042026	38.0	35.0	40.0	31.0	41.0
31	36.32333721494645	38.0	35.0	40.0	30.0	41.0
32	35.80555471955538	38.0	35.0	40.0	27.0	41.0
33	35.355786883234444	38.0	35.0	41.0	24.0	41.0
34	34.97910758396207	38.0	35.0	41.0	21.0	41.0
35	34.63080611766921	38.0	34.0	41.0	18.0	41.0
36	34.45560414645797	38.0	34.0	40.0	18.0	41.0
37	34.4060881133894	38.0	34.0	40.0	18.0	41.0
38	34.28201539835151	38.0	34.0	40.0	17.0	41.0
39	34.178143662900034	38.0	34.0	40.0	16.0	41.0
40	34.11501651807728	38.0	33.0	40.0	15.0	41.0
41	34.0904848003593	38.0	34.0	40.0	15.0	41.0
42	33.81736470108878	38.0	33.0	40.0	15.0	41.0
43	33.85473236547664	38.0	33.0	40.0	15.0	41.0
44	33.639680966899796	38.0	33.0	40.0	13.0	41.0
45	33.467987495495244	38.0	33.0	40.0	12.0	41.0
46	33.546635305084756	38.0	33.0	40.0	13.0	41.0
47	33.43335818183081	38.0	33.0	40.0	12.0	41.0
48	33.42182369450856	38.0	33.0	40.0	12.0	41.0
49	33.349553151476286	38.0	33.0	40.0	12.0	41.0
50	32.88966032412627	37.0	32.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	1.0
13	3.0
14	10.0
15	47.0
16	188.0
17	444.0
18	1274.0
19	2796.0
20	5350.0
21	8858.0
22	13973.0
23	21282.0
24	32571.0
25	50608.0
26	68715.0
27	72424.0
28	65789.0
29	61419.0
30	65103.0
31	75998.0
32	94425.0
33	119372.0
34	169605.0
35	243705.0
36	236547.0
37	347827.0
38	406418.0
39	426950.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.054337181763675	22.684170195230475	27.59286740085488	20.66862522215097
2	37.48183628852964	23.268457147531393	27.063216275302832	12.186490288636135
3	25.121136425196376	23.251865759465	39.23485148392603	12.39214633141259
4	22.20089778701751	25.54471842099374	38.96475912005451	13.289624671934238
5	20.07685285290847	29.83556005195034	37.577757662327436	12.509829432813751
6	17.85503448307794	37.48608059710477	34.65439366965234	10.00449125016495
7	79.59838037184774	3.13758582184862	15.444421550901222	1.8196122554024259
8	49.35926374364993	39.75068931429722	9.152388426773715	1.7376585152791253
9	74.6454266031718	5.002727932875102	17.338116283251264	3.0137291807018234
10	34.17706329344455	30.35494766767527	23.871148965198984	11.596840073681197
11	26.530092533643863	24.178552659908185	34.89030005718241	14.40105474926554
12	24.296197176685936	22.175393350943356	36.94605020785537	16.582359264515343
13	19.556925052455796	27.775449838832028	37.678695037168566	14.988930071543608
14	16.786394753108567	30.4913828960538	36.574441699791564	16.147780651046066
15	15.668636797537994	24.388877442117277	44.63839648478647	15.30408927555826
16	17.07462188998289	23.01487900247945	42.85250719024457	17.057991917293087
17	17.826520446377792	23.29087481373273	36.59539315030331	22.287211589586164
18	19.761192048789486	23.511848952003046	38.920502557003	17.806456442204478
19	22.493677909454235	26.00480147053717	34.11146943364718	17.390051186361415
20	23.160690294346658	24.24692461259109	34.508968224019235	18.083416869043017
21	20.53330894785134	26.929752062926894	35.18605119562173	17.350887793600045
22	19.849859513386164	24.089036333596482	34.698495894210225	21.362608258807132
23	17.633520160079886	27.78224073255223	34.55766201876293	20.02657708860496
24	17.538524817243932	24.91953176787799	39.14981097392992	18.392132440948163
25	17.302772768207504	26.492858372053004	37.47172711719616	18.73264174254333
26	17.279197563303864	28.678291442007698	35.72392084596015	18.31859014872829
27	18.099275149264614	27.817622832219396	35.65909867863098	18.424003339885004
28	17.38345321575827	26.54861315288077	39.24229831624421	16.82563531511676
29	17.981630632486866	24.729734005323134	38.14460436484694	19.144030997343062
30	18.98926807284468	26.564471433102366	36.050462513880824	18.395797980172134
31	21.503905149735346	25.81974961666177	33.377821404125314	19.29852382947757
32	22.78950621714037	25.814386354007745	33.47790991725142	17.918197511600468
33	21.148077752646326	27.55509305453628	32.28240394550925	19.014425247308147
34	18.99968592116544	27.36726310777534	33.38978263738248	20.24326833367674
35	18.703085921011102	27.222609354610437	33.470270161816195	20.604034562562266
36	22.169489903561594	27.24263477416034	31.37581963386279	19.212055688415276
37	19.9447776869753	29.155698987462316	31.50530963002748	19.39421369553491
38	19.062578857324095	29.982567467143262	30.00510088721483	20.94975278831781
39	20.264412707305535	28.967907625324784	29.912266283289853	20.855413384079828
40	21.485963299849598	27.002213985691277	29.903353235282086	21.608469479177035
41	19.432836903568536	26.404538169067017	29.99985337843104	24.162771548933407
42	20.99188719708177	27.507055198390557	29.251157345779188	22.249900258748482
43	20.648599802601066	27.673470679158825	30.47247643058279	21.205453087657318
44	20.303460346196676	29.369920816635837	30.50276535995981	19.823853477207678
45	18.791676216360962	30.333340278565547	29.982914728753958	20.892068776319537
46	20.81061663630057	29.506317460390957	29.25679070079708	20.426275202511395
47	20.22038765199448	27.38466477293335	30.511871331084624	21.88307624398755
48	20.637873277293025	26.331265969211014	31.033072424109832	21.997788329386125
49	20.527096823482292	25.669308169985328	31.511714677513574	22.291880329018802
50	19.30218936870154	27.812954092786757	31.33638614873755	21.54847038977415
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1160.0
1	1759.5
2	2359.0
3	31859.0
4	61359.0
5	45866.5
6	30374.0
7	31262.0
8	32150.0
9	33216.5
10	34283.0
11	34469.5
12	34656.0
13	34006.5
14	33357.0
15	31845.5
16	30334.0
17	28763.0
18	27192.0
19	26025.0
20	24858.0
21	24592.0
22	24326.0
23	25016.0
24	25706.0
25	27834.0
26	29962.0
27	33613.0
28	37264.0
29	42562.0
30	47860.0
31	54263.5
32	60667.0
33	68282.5
34	75898.0
35	85426.0
36	94954.0
37	104459.0
38	113964.0
39	129998.0
40	146032.0
41	165074.5
42	184117.0
43	197818.0
44	211519.0
45	221808.5
46	232098.0
47	231795.0
48	231492.0
49	217766.0
50	204040.0
51	178617.5
52	153195.0
53	134595.5
54	115996.0
55	101675.5
56	87355.0
57	77592.0
58	67829.0
59	59439.0
60	51049.0
61	43065.0
62	35081.0
63	28178.0
64	21275.0
65	16476.0
66	11677.0
67	9311.0
68	6945.0
69	5510.0
70	4075.0
71	3243.0
72	2411.0
73	1898.0
74	1385.0
75	1025.5
76	666.0
77	517.0
78	368.0
79	293.5
80	219.0
81	177.0
82	135.0
83	90.0
84	45.0
85	29.5
86	14.0
87	8.0
88	2.0
89	2.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2591706.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.92172349395145
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.91942291393846	29.549147864766745
2	7.805859643475688	6.076350213519103
3	3.511485129482386	4.100191597885072
4	2.2264601321256525	3.4663066253160504
5	1.5889561807877453	3.0922456556312876
6	1.207693810664042	2.820331473841343
7	0.9466928017620708	2.579284082472826
8	0.8152327668629522	2.538421146803905
9	0.6847341983532489	2.3985931621641887
>10	5.149235686689916	37.007288527313456
>50	0.1255553316322939	3.057421907822664
>100	0.01628824564212725	1.1207232200800017
>500	0.0016578494493027234	0.45765319312457003
>1k	5.180779529069495E-4	0.32166389393380185
>5k	0.0	0.0
>10k+	2.072311811627798E-4	1.4143774353250207
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21714	0.8378265127294532	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13357	0.5153748148902692	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07385096920715545	0.0
2	0.0	0.0	0.0	0.20426699633368908	0.0
3	0.0	0.0	0.0	0.34672142596421046	0.0
4	0.0	0.0	0.0	0.4681086512127533	0.0
5	0.0	0.0	0.0	0.7246963968906968	0.0
6	0.0	0.0	0.0	1.2348622876205866	0.0
7	0.0	0.0	0.0	1.6065093803077972	0.0
8	0.0	0.0	0.0	2.469917498358224	0.0
9	0.0	0.0	0.0	3.3594859910807786	0.0
10	0.0	0.0	0.0	4.565679903507574	0.0
11	0.0	0.0	0.0	5.168255967304933	0.0
12	0.0	0.0	0.0	5.792130743224733	0.0
13	0.0	0.0	0.0	6.167366205889094	0.0
14	0.0	0.0	0.0	6.349408459138498	0.0
15	0.0	0.0	0.0	6.507412492003337	0.0
16	0.0	0.0	0.0	6.770675377531248	0.0
17	0.0	0.0	0.0	7.061140422563362	0.0
18	0.0	0.0	0.0	7.568142374173614	0.0
19	0.0	0.0	0.0	7.75759287511778	0.0
20	0.0	0.0	0.0	7.996817540261126	0.0
21	0.0	0.0	0.0	8.214897831775673	0.0
22	0.0	0.0	0.0	8.44362747935144	0.0
23	2.3150774046130232E-4	3.858462341021705E-5	0.0	8.709244026907372	0.0
24	2.3150774046130232E-4	3.858462341021705E-5	0.0	8.914205546462446	0.0
25	2.3150774046130232E-4	3.858462341021705E-5	0.0	9.096595061322542	0.0
26	2.3150774046130232E-4	3.858462341021705E-5	0.0	9.267370604536163	0.0
27	2.3150774046130232E-4	3.858462341021705E-5	0.0	9.480897910488304	0.0
28	2.3150774046130232E-4	3.858462341021705E-5	0.0	9.676599120424925	0.0
29	2.3150774046130232E-4	3.858462341021705E-5	0.0	9.885033256086917	0.0
30	2.3150774046130232E-4	3.858462341021705E-5	0.0	10.165427714408965	0.0
31	2.3150774046130232E-4	3.858462341021705E-5	0.0	10.388871268577532	0.0
32	2.3150774046130232E-4	3.858462341021705E-5	0.0	10.600315004865521	0.0
33	2.3150774046130232E-4	3.858462341021705E-5	0.0	10.814806926402918	0.0
34	2.3150774046130232E-4	3.858462341021705E-5	0.0	11.02513170861201	0.0
35	2.3150774046130232E-4	3.858462341021705E-5	0.0	11.251584863406574	0.0
36	2.3150774046130232E-4	3.858462341021705E-5	0.0	11.469047800946559	0.0
37	2.3150774046130232E-4	3.858462341021705E-5	0.0	11.695693878858172	0.0
38	2.3150774046130232E-4	3.858462341021705E-5	0.0	11.922147033652736	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGA	20	7.858313E-4	44.0	2
CGTTTTT	31895	0.0	43.510265	1
GAGCGAT	1670	0.0	41.628746	7
GTTTTTA	14125	0.0	40.666904	2
CGACGTC	95	0.0	39.36842	18
TCGTAGA	120	0.0	38.5	2
GAGACCG	675	0.0	37.807404	7
CGAGGTT	175	0.0	37.714287	6
AGCGATA	435	0.0	37.42529	8
AGACCGA	600	0.0	37.033333	8
CGACGGT	180	0.0	36.666668	28
CGACGAA	30	1.3015464E-4	36.666664	22
TACGAGA	475	0.0	36.126316	4
GTTTTTT	22970	0.0	36.088814	2
GACCGAT	1335	0.0	35.7603	9
CGGTCTA	185	0.0	35.67568	31
TATTTTT	16230	0.0	35.636475	7
ACGTCGC	105	0.0	35.61905	20
ACGGGAT	890	0.0	35.595505	5
GCGCGAC	260	0.0	35.538464	9
>>END_MODULE