FastQCFastQC Report
Thu 26 May 2016
SRR1546755_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546755_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2220900
Sequences flagged as poor quality0
Sequence length50
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181320.8164257733351344No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114980.5177180422351298No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACGG254.4441855E-544.02
CGTTTTT262700.043.3719061
GAGCGAT13500.042.3703737
TGCGCGA800.041.252
CTTACGA700.040.8571432
TTAAGCG1000.039.6000021
GTTTTTA116350.039.4997832
AGCGATA3200.039.18758
CGAACGG452.3526809E-839.111112
AGCGATC2150.037.8604668
CGACGGT1400.037.71428728
CGCGCGA357.2932744E-637.7142872
CGAAGCG357.2932744E-637.7142871
CGGTCTA1350.037.4814831
GACCGAT9400.037.4468089
GCGATCT2950.037.2881369
CGAGGTT2350.036.5106356
AAGGGCG5500.036.45
CGAGATT4000.036.3000036
ACGGTCT1400.036.14285730
Produced by FastQC (version 0.11.5)