##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1546755_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2220900 Sequences flagged as poor quality 0 Sequence length 50 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.549515511729478 31.0 31.0 33.0 30.0 34.0 2 31.94144806159665 31.0 31.0 34.0 30.0 34.0 3 32.00343599441668 33.0 31.0 34.0 30.0 34.0 4 35.72570894682336 37.0 35.0 37.0 35.0 37.0 5 35.63075825115944 37.0 35.0 37.0 33.0 37.0 6 35.67896933675537 37.0 35.0 37.0 33.0 37.0 7 36.07021747940024 37.0 35.0 37.0 35.0 37.0 8 22.06171146832365 35.0 0.0 37.0 0.0 37.0 9 29.95323922734027 37.0 18.0 39.0 17.0 39.0 10 34.39584357692827 35.0 32.0 39.0 27.0 39.0 11 36.031677698230446 37.0 35.0 39.0 32.0 39.0 12 36.654477013823225 38.0 35.0 39.0 33.0 39.0 13 36.86710162546715 39.0 35.0 39.0 33.0 39.0 14 38.07479040028817 40.0 37.0 41.0 33.0 41.0 15 38.2698419559638 40.0 37.0 41.0 33.0 41.0 16 38.270392183349095 40.0 37.0 41.0 33.0 41.0 17 38.20025124949345 40.0 37.0 41.0 33.0 41.0 18 38.08578324102841 40.0 37.0 41.0 33.0 41.0 19 38.02848439821694 40.0 37.0 41.0 34.0 41.0 20 37.86242244135261 40.0 36.0 41.0 33.0 41.0 21 37.79233418884236 40.0 35.0 41.0 33.0 41.0 22 37.76967715790896 40.0 35.0 41.0 33.0 41.0 23 37.581415192039266 39.0 35.0 41.0 33.0 41.0 24 37.382427844567516 39.0 35.0 41.0 33.0 41.0 25 37.25062542212616 39.0 35.0 41.0 33.0 41.0 26 37.2010864964654 39.0 35.0 41.0 33.0 41.0 27 37.09615426178576 39.0 35.0 41.0 32.0 41.0 28 37.07046647755414 39.0 35.0 41.0 32.0 41.0 29 36.95686253320726 39.0 35.0 41.0 32.0 41.0 30 36.800994641811876 39.0 35.0 41.0 31.0 41.0 31 36.59760232338241 39.0 35.0 41.0 31.0 41.0 32 36.124864694493226 39.0 35.0 41.0 30.0 41.0 33 35.71297492007744 39.0 35.0 41.0 25.0 41.0 34 35.340139583051915 39.0 35.0 41.0 23.0 41.0 35 35.02006033589986 39.0 35.0 41.0 21.0 41.0 36 34.85652708361475 39.0 35.0 41.0 20.0 41.0 37 34.83131072988428 39.0 35.0 41.0 19.0 41.0 38 34.71995767481651 39.0 34.0 41.0 18.0 41.0 39 34.62864919627178 39.0 34.0 41.0 18.0 41.0 40 34.578587509568194 39.0 34.0 40.0 18.0 41.0 41 34.55835517132694 39.0 34.0 40.0 18.0 41.0 42 34.335347831960014 38.0 34.0 40.0 17.0 41.0 43 34.36179431761899 38.0 34.0 40.0 17.0 41.0 44 34.17768877482102 38.0 34.0 40.0 16.0 41.0 45 33.97493943896618 38.0 33.0 40.0 15.0 41.0 46 34.035850781214826 38.0 33.0 40.0 15.0 41.0 47 33.9239492998334 38.0 33.0 40.0 15.0 41.0 48 33.91451933900671 38.0 33.0 40.0 15.0 41.0 49 33.83313836732856 38.0 33.0 40.0 15.0 41.0 50 33.40863613850241 37.0 33.0 40.0 14.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 0.0 10 1.0 11 3.0 12 4.0 13 3.0 14 12.0 15 30.0 16 107.0 17 308.0 18 788.0 19 1817.0 20 3622.0 21 6337.0 22 10101.0 23 15584.0 24 24091.0 25 38836.0 26 53281.0 27 57985.0 28 52953.0 29 49716.0 30 52344.0 31 60833.0 32 76027.0 33 97738.0 34 141098.0 35 200292.0 36 202274.0 37 304452.0 38 367020.0 39 403241.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 30.124409023368905 22.8329055788194 26.365797649601515 20.676887748210184 2 36.944481966770226 23.483542707911205 27.190418298887835 12.381557026430725 3 25.761583141969474 23.459228240803277 38.303975865640055 12.475212751587195 4 22.52132018551038 25.671529560088253 38.52172542662885 13.285424827772523 5 20.87955333423387 29.813679139087757 36.62704309063893 12.679724436039445 6 18.28646044396416 37.765140258453776 33.87401503894817 10.074384258633886 7 80.71128821648881 2.9371876266378494 14.66909811337746 1.6824260434958802 8 50.04182988878383 39.789454725561704 8.57021928047188 1.5984961051825837 9 75.60272862353101 5.12999234544554 16.28137241658787 2.985906614435589 10 33.46719798279977 33.29492548066099 22.247827457337117 10.990049079202127 11 25.42406231707866 24.520464676482508 36.025350083299564 14.030122923139269 12 24.09149443919132 22.47404205502274 36.78643793056869 16.648025575217254 13 19.976676122292762 26.315142509793326 37.98131388175965 15.726867486154262 14 17.06853077581161 29.394569769012563 36.803142870007655 16.733756585168173 15 16.367463640866315 24.801071637624386 43.4946192984826 15.336845423026702 16 18.47849970732586 24.143005088027376 41.49493448601918 15.883560718627585 17 18.780809581701114 23.728443423837184 36.8585258228646 20.632221171597102 18 20.29839254356342 24.061686703588634 38.16587869782521 17.47404205502274 19 22.107388896393353 25.92791210770408 34.7516322211716 17.213066774730965 20 23.680850105812958 24.771263902021705 34.1450312936197 17.40285469854563 21 21.413390967625737 26.579990094105995 35.07578008915305 16.930838849115222 22 20.362060425953445 24.560268359674005 35.14462605250124 19.933045161871313 23 18.099869422306273 27.584627853572876 35.16713944797154 19.148363276149308 24 18.493133414381557 25.051510648836057 38.358503309469135 18.09685262731325 25 17.94538250258904 26.53095591877167 36.883785852582285 18.639875726057003 26 17.847584312666037 28.362555720653788 35.79040028817146 17.99945967850871 27 18.281957764870096 27.04673780899635 36.02980773560268 18.641496690530865 28 17.55387455536044 27.08924309964429 38.53892566076816 16.817956684227113 29 18.834706650457022 25.02345895808006 37.34490521860507 18.79692917285785 30 19.767166464046106 25.69647440226935 36.30789319645189 18.228465937232656 31 22.528344364897112 25.416002521500292 33.31271106308253 18.74294205052006 32 22.596965194290604 26.248142644873703 34.1582241433653 16.996668017470395 33 22.785267234004234 26.870277815300103 32.28479445269936 18.05966049799631 34 20.744517988202983 27.775001125669775 33.29551082894322 18.184970057184024 35 21.49128731595299 27.541672295015534 32.18078256562655 18.786257823404924 36 23.267864379305685 28.83848890089603 30.48682966364987 17.40681705614841 37 22.856499617272277 29.588365077220946 30.302805169075597 17.252330136431176 38 21.706875590976633 30.619388536179027 29.245125849880676 18.428610022963664 39 21.5626998063848 29.99500202620559 29.575847629339457 18.86645053807015 40 21.93705254626503 28.57850421000495 30.38497906254221 19.099464181187805 41 19.3162231527759 28.213246881894726 30.399252555270383 22.071277410058983 42 21.188662254041155 28.626682876311406 29.393083884911526 20.79157098473592 43 21.392048268719886 28.249223287856275 29.975415372146426 20.38331307127741 44 20.868386690080598 29.45296951686253 30.426223603043812 19.25242019001306 45 20.2687649151245 29.676437480300777 29.871538565446443 20.18325903912828 46 21.659192219370528 29.592507541987484 29.329506056103384 19.41879418253861 47 21.168625332072583 27.79395740465577 30.55022738529425 20.487189877977396 48 21.264262236030437 26.906839569543877 30.910891980728533 20.918006213697147 49 21.282363005988564 25.96902156783286 31.09892385969652 21.64969156648206 50 20.109820343104147 27.87198883335585 31.231392678643793 20.786798144896213 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 978.0 1 1572.0 2 2166.0 3 28520.5 4 54875.0 5 40804.5 6 26734.0 7 27106.0 8 27478.0 9 27865.0 10 28252.0 11 28056.5 12 27861.0 13 26835.0 14 25809.0 15 24682.5 16 23556.0 17 22379.0 18 21202.0 19 20401.5 20 19601.0 21 19368.5 22 19136.0 23 19852.5 24 20569.0 25 23411.0 26 26253.0 27 30815.5 28 35378.0 29 40492.0 30 45606.0 31 52464.5 32 59323.0 33 66054.0 34 72785.0 35 79233.0 36 85681.0 37 93972.5 38 102264.0 39 114352.0 40 126440.0 41 140230.0 42 154020.0 43 164049.5 44 174079.0 45 182054.5 46 190030.0 47 189991.0 48 189952.0 49 179051.5 50 168151.0 51 149102.0 52 130053.0 53 114611.0 54 99169.0 55 87545.0 56 75921.0 57 68905.0 58 61889.0 59 55170.5 60 48452.0 61 41342.0 62 34232.0 63 27066.0 64 19900.0 65 15193.5 66 10487.0 67 7926.0 68 5365.0 69 4239.0 70 3113.0 71 2489.0 72 1865.0 73 1454.5 74 1044.0 75 869.5 76 695.0 77 514.0 78 333.0 79 227.5 80 122.0 81 82.0 82 42.0 83 31.0 84 20.0 85 14.0 86 8.0 87 6.0 88 4.0 89 4.0 90 4.0 91 3.0 92 2.0 93 1.0 94 0.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 2220900.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.45131482332832 #Duplication Level Percentage of deduplicated Percentage of total 1 71.99204016622348 27.681886012051564 2 7.988788444121093 6.143588390437347 3 4.245592943643092 4.897458926631653 4 2.8921231589763665 4.448237523745564 5 2.1911019027663077 4.21253745366305 6 1.7539469214403125 4.046493915582537 7 1.3674409546535335 3.680593185478695 8 1.1468112734831153 3.5277121055713065 9 0.9218365667063725 3.1901245237874543 >10 5.442480827068598 34.094539980778116 >50 0.04236094101589145 1.074087987048884 >100 0.0135114556727223 1.0270627375403296 >500 0.0013505554075197012 0.38409783193180796 >1k 3.6833329295991814E-4 0.1927570609362796 >5k 0.0 0.0 >10k+ 2.4555552863994546E-4 1.398822364815568 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18132 0.8164257733351344 No Hit CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11498 0.5177180422351298 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0629024269440317 0.0 2 0.0 0.0 0.0 0.1812328335359539 0.0 3 0.0 0.0 0.0 0.3001035616191634 0.0 4 0.0 0.0 0.0 0.41222027106128145 0.0 5 0.0 0.0 0.0 0.6433427889594309 0.0 6 0.0 0.0 0.0 1.080057634292404 0.0 7 0.0 0.0 0.0 1.3986221801972174 0.0 8 0.0 0.0 0.0 2.1692106803548112 0.0 9 0.0 0.0 0.0 2.9830699266063307 0.0 10 0.0 0.0 0.0 4.0248547885992165 0.0 11 0.0 0.0 0.0 4.514205952541762 0.0 12 0.0 0.0 0.0 5.014543653473817 0.0 13 0.0 0.0 0.0 5.3198252960511505 0.0 14 0.0 0.0 0.0 5.463370705569814 0.0 15 0.0 0.0 0.0 5.592237381241839 0.0 16 0.0 0.0 0.0 5.79544328875681 0.0 17 0.0 0.0 0.0 6.016840019811788 0.0 18 0.0 0.0 0.0 6.390292223873205 0.0 19 0.0 0.0 0.0 6.544013688144446 0.0 20 0.0 0.0 0.0 6.742311675446891 0.0 21 0.0 0.0 0.0 6.903687694178036 0.0 22 0.0 0.0 0.0 7.0786167769823045 0.0 23 0.0 0.0 0.0 7.282948354270791 0.0 24 0.0 0.0 0.0 7.43869602413436 0.0 25 0.0 0.0 0.0 7.577783781349903 0.0 26 0.0 0.0 0.0 7.7199783871403485 0.0 27 0.0 0.0 0.0 7.88995452294115 0.0 28 0.0 0.0 0.0 8.048178666306452 0.0 29 4.5026790940609664E-5 0.0 0.0 8.207753613399973 0.0 30 4.5026790940609664E-5 0.0 0.0 8.418569048583908 0.0 31 4.5026790940609664E-5 0.0 0.0 8.589670854158225 0.0 32 4.5026790940609664E-5 0.0 0.0 8.76000720428655 0.0 33 4.5026790940609664E-5 0.0 0.0 8.927777027331262 0.0 34 4.5026790940609664E-5 0.0 0.0 9.089513260389932 0.0 35 4.5026790940609664E-5 0.0 0.0 9.263766941330092 0.0 36 4.5026790940609664E-5 0.0 0.0 9.440271961817281 0.0 37 4.5026790940609664E-5 0.0 0.0 9.623080733036156 0.0 38 4.5026790940609664E-5 0.0 0.0 9.817281282363005 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCACGG 25 4.4441855E-5 44.0 2 CGTTTTT 26270 0.0 43.371906 1 GAGCGAT 1350 0.0 42.370373 7 TGCGCGA 80 0.0 41.25 2 CTTACGA 70 0.0 40.857143 2 TTAAGCG 100 0.0 39.600002 1 GTTTTTA 11635 0.0 39.499783 2 AGCGATA 320 0.0 39.1875 8 CGAACGG 45 2.3526809E-8 39.11111 2 AGCGATC 215 0.0 37.860466 8 CGACGGT 140 0.0 37.714287 28 CGCGCGA 35 7.2932744E-6 37.714287 2 CGAAGCG 35 7.2932744E-6 37.714287 1 CGGTCTA 135 0.0 37.48148 31 GACCGAT 940 0.0 37.446808 9 GCGATCT 295 0.0 37.288136 9 CGAGGTT 235 0.0 36.510635 6 AAGGGCG 550 0.0 36.4 5 CGAGATT 400 0.0 36.300003 6 ACGGTCT 140 0.0 36.142857 30 >>END_MODULE