FastQCFastQC Report
Thu 26 May 2016
SRR1546754_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546754_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2488182
Sequences flagged as poor quality0
Sequence length50
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199420.8014687028521225No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124340.4997222871960331No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA35220.14154913105231048No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAC207.8582525E-444.043
CCGAACG207.8582525E-444.01
CGTTTTT246200.043.3208771
CGACGGT2150.041.9534928
GAGCGAT16750.041.110457
GTTTTTA111100.039.7821772
CGTACAA404.1271232E-738.536
AGCGATA4700.037.914898
TGCGCGA700.037.7142872
GCGTTAG1850.036.8648641
CACGACG2450.036.81632626
CGGTCTA2450.036.81632631
ACGTCGC1200.036.66666420
GCGCGAC2500.036.089
GACCGAT14300.035.538469
CGAGATT4300.035.3023266
GTTAGCG1500.035.21
GTTTTTT182150.035.1951682
GACGGTC2600.034.6923129
GTATACG1650.034.6666681
Produced by FastQC (version 0.11.5)