FastQCFastQC Report
Thu 26 May 2016
SRR1546753_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546753_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4050629
Sequences flagged as poor quality0
Sequence length50
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224860.5551236610412852No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139620.3446872078385851No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA46590.11501917356538946No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCGA207.8588387E-444.013
CGACGGT4950.043.55555728
CGTTTTT309250.042.9684751
GAGCGAT28850.041.2547657
CTTGCGA1350.040.740742
CACGACG5400.040.33333226
AGCGATA6750.040.0888868
CGGTCTA5450.039.55962831
GTTGCGA1300.038.9230772
TCACGAC5900.038.03389725
GTTTTTA142600.037.767182
ACGACGG5800.037.55172727
GACCGAT20250.036.9382749
GACGGTC5850.036.854729
GCGCGAC8250.036.89
CGCGTAG301.3016755E-436.6666641
CGTAAGG4200.036.1428572
GTTTACG1900.035.8947371
GCGTAAC1300.035.5384632
TACGGGA11750.035.0127684
Produced by FastQC (version 0.11.5)