FastQCFastQC Report
Thu 26 May 2016
SRR1546752_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546752_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2709620
Sequences flagged as poor quality0
Sequence length50
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192940.7120555649869724No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119580.44131649456381333No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA31300.11551435256604246No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAGT502.7284841E-1144.04
CGTTTTT282800.043.253181
GAGCGAT17850.041.5350157
CGACGGT3250.040.61538728
TCGCGCG603.6379788E-1240.3333361
CGATCGA501.3496901E-939.610
GTTTTTA127600.039.5172422
CGCGCGA452.3530447E-839.111112
TCACGAC3350.038.7462725
GCCGATT800.038.5000049
GTTAGCG1500.038.133331
GCGATCG1350.037.481489
GCGCGAC3950.037.3164569
TATCGCG950.037.052631
TATACGA1250.036.962
AGACCGA6050.036.7272728
GACCGAT14400.036.513899
ACGAGAT3500.036.457145
CGTAAGA1450.036.413792
AGCGATC2250.036.177788
Produced by FastQC (version 0.11.5)