##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546752_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2709620
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.44946745300079	31.0	31.0	33.0	30.0	34.0
2	31.858841830219735	31.0	31.0	34.0	30.0	34.0
3	31.940534097032057	31.0	31.0	34.0	30.0	34.0
4	35.66664513843269	37.0	35.0	37.0	33.0	37.0
5	35.555608904569645	37.0	35.0	37.0	33.0	37.0
6	35.608689410323215	37.0	35.0	37.0	33.0	37.0
7	36.011167986654954	37.0	35.0	37.0	35.0	37.0
8	21.977607192152405	35.0	0.0	37.0	0.0	37.0
9	29.885486525785904	37.0	18.0	39.0	17.0	39.0
10	34.20034580494682	35.0	32.0	39.0	27.0	39.0
11	35.89020083997018	37.0	35.0	39.0	32.0	39.0
12	36.46354322746363	37.0	35.0	39.0	32.0	39.0
13	36.660170429801965	39.0	35.0	39.0	33.0	39.0
14	37.82951114916483	40.0	36.0	41.0	33.0	41.0
15	38.05936404366664	40.0	37.0	41.0	33.0	41.0
16	38.077023346447106	40.0	37.0	41.0	33.0	41.0
17	38.03736464891756	40.0	36.0	41.0	33.0	41.0
18	37.94660136845757	39.0	36.0	41.0	33.0	41.0
19	37.896153335154004	40.0	36.0	41.0	33.0	41.0
20	37.729466124401206	40.0	35.0	41.0	33.0	41.0
21	37.64357437574272	39.0	35.0	41.0	33.0	41.0
22	37.61020032329257	39.0	35.0	41.0	33.0	41.0
23	37.428857182926016	39.0	35.0	41.0	33.0	41.0
24	37.242734774617844	39.0	35.0	41.0	33.0	41.0
25	37.10799189554255	39.0	35.0	41.0	32.0	41.0
26	37.0475834249821	39.0	35.0	41.0	32.0	41.0
27	36.922075789225055	39.0	35.0	41.0	32.0	41.0
28	36.896598046958616	39.0	35.0	41.0	32.0	41.0
29	36.789283368147565	39.0	35.0	41.0	31.0	41.0
30	36.63669555140573	38.0	35.0	40.0	31.0	41.0
31	36.47102951705405	38.0	35.0	40.0	31.0	41.0
32	35.99260560521402	38.0	35.0	41.0	29.0	41.0
33	35.63563377890627	39.0	35.0	41.0	25.0	41.0
34	35.33396601737513	39.0	35.0	41.0	24.0	41.0
35	35.040903152471564	39.0	35.0	41.0	22.0	41.0
36	34.87738096116799	38.0	35.0	41.0	21.0	41.0
37	34.83088182106716	38.0	34.0	40.0	21.0	41.0
38	34.72073132025893	38.0	34.0	40.0	20.0	41.0
39	34.638521637720416	38.0	34.0	40.0	19.0	41.0
40	34.551684369025914	38.0	34.0	40.0	18.0	41.0
41	34.553731888604304	38.0	34.0	40.0	18.0	41.0
42	34.29547870181059	38.0	34.0	40.0	18.0	41.0
43	34.357238284335075	38.0	34.0	40.0	18.0	41.0
44	34.17498911286454	38.0	34.0	40.0	18.0	41.0
45	33.98847513673504	38.0	33.0	40.0	17.0	41.0
46	34.07923103608624	38.0	33.0	40.0	18.0	41.0
47	33.96399199887807	38.0	33.0	40.0	17.0	41.0
48	33.96396948649626	38.0	33.0	40.0	18.0	41.0
49	33.888478089178555	38.0	33.0	40.0	17.0	41.0
50	33.44034329536983	37.0	33.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	6.0
12	5.0
13	4.0
14	11.0
15	44.0
16	141.0
17	461.0
18	1129.0
19	2672.0
20	5015.0
21	8212.0
22	13224.0
23	20344.0
24	30785.0
25	46370.0
26	62506.0
27	67010.0
28	62332.0
29	59961.0
30	65195.0
31	78240.0
32	97683.0
33	125775.0
34	182830.0
35	269523.0
36	248372.0
37	368155.0
38	436301.0
39	457312.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.715244203984327	23.892649153755876	28.83777060990102	18.55433603235878
2	35.37315195488666	24.064186122039253	28.068917412773747	12.493744510300338
3	25.580413489714427	23.779496756002686	37.55522914652239	13.084860607760499
4	22.92723702954658	26.016083436053766	37.4829680914667	13.573711442932957
5	20.979362419822706	30.132048036256005	36.16702711081259	12.721562433108701
6	18.301200906400158	38.46568891578893	32.89191842398565	10.34119175382526
7	81.80224533329397	3.232593500195599	13.26311438504292	1.702046781467512
8	50.736007263011054	39.981990094552	7.665207667495812	1.6167949749411357
9	76.88328990780995	5.1133738310168955	15.08359105704859	2.9197452041245637
10	35.8018467534193	29.042338040020372	23.16893143688045	11.986883769679881
11	29.076992345790188	24.858762483300243	31.57656793203475	14.487677238874824
12	25.866210022069513	22.302204737195623	35.31166731866461	16.519917922070253
13	19.895225160723644	29.81274126999358	35.58270163343937	14.709331935843403
14	16.963854710254576	32.43273964614964	34.88404278090655	15.719362862689234
15	15.70788523852053	24.997047556483935	44.258825960835836	15.036241244159697
16	16.90229626294462	23.19535580634923	42.294602195141756	17.607745735564396
17	17.594865700725563	23.907226843616446	35.09879614115633	23.399111314501663
18	19.63419224835955	24.385559598762928	37.80847498911287	18.17177316376466
19	22.650814505354994	26.69876218805589	32.909005690834874	17.741417615754237
20	24.03957012422406	24.54886663074527	33.17922808364273	18.232335161387944
21	20.6428576700792	27.64247385242211	34.06846716513755	17.646201312361143
22	20.340933415017602	24.922092396719837	32.99252293679557	21.744451251466995
23	17.664063595633337	28.938264406079085	32.7397937718204	20.65787822646718
24	17.766255046833134	25.63226577896532	38.57341619858135	18.028062975620198
25	16.532170562661925	27.533196536783755	37.10180763354271	18.83282526701161
26	17.18488201297599	29.855662417608375	34.20534982765111	18.75410574176453
27	18.17550062370369	28.95808268318067	34.074187524449925	18.792229168665717
28	17.037997948051757	27.674803108923022	38.240159136705515	17.047039806319706
29	17.321580147769797	25.24080867427905	36.91539773104716	20.522213446903994
30	18.42752858334379	27.674692392291174	34.33307991526487	19.564699109100168
31	21.487736287745147	26.714077988795477	31.42185989179295	20.376325831666435
32	22.072098670662307	26.9764395007418	31.628826182269098	19.32263564632679
33	21.335168769052487	28.015404374045065	30.0979104081015	20.55151644880094
34	18.447826632516737	29.147445029192287	30.505532141038227	21.899196197252753
35	18.87832980270296	28.9551302396646	30.60691167027111	21.559628287361328
36	22.848185354403938	29.63869472472155	28.484030971132484	19.02908894974203
37	20.39437264265838	31.402779725570372	29.66925251511282	18.533595116658425
38	19.729002590769184	32.72056598342203	28.216539588576993	19.33389183723179
39	20.992832943364753	30.70412087303755	29.027132955912638	19.275913227685063
40	21.80962644208413	28.600357245665442	28.8164761110414	20.773540201209027
41	19.25856762202818	27.16443634162724	29.465976779031745	24.111019257312833
42	20.7770093223404	28.197976099969736	28.340062444180365	22.684952133509494
43	20.3908297104391	28.036625061816782	29.643160295539595	21.929384932204517
44	20.22217137458389	30.16659162539397	29.780264391316862	19.83097260870528
45	18.708748828248982	31.8376377499428	28.53518205504831	20.918431366759915
46	21.03173138668891	30.50018083716536	28.632944840974012	19.83514293517172
47	20.53893165831371	28.044670470398064	29.7319919398292	21.684405931459025
48	20.858718196647498	27.08125124556211	30.071116983193214	21.988913574597177
49	20.87362803640363	26.102368597810766	30.716078269277613	22.307925096507997
50	19.170547899705497	29.358950701574386	30.080564802444627	21.38993659627549
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1367.0
1	1902.0
2	2437.0
3	28151.5
4	53866.0
5	40329.5
6	26793.0
7	27471.0
8	28149.0
9	29360.0
10	30571.0
11	30844.5
12	31118.0
13	30552.5
14	29987.0
15	28659.5
16	27332.0
17	25984.0
18	24636.0
19	23705.0
20	22774.0
21	22982.5
22	23191.0
23	24205.0
24	25219.0
25	27931.5
26	30644.0
27	35227.5
28	39811.0
29	45457.0
30	51103.0
31	59524.0
32	67945.0
33	77417.0
34	86889.0
35	95503.5
36	104118.0
37	114330.5
38	124543.0
39	142993.5
40	161444.0
41	183520.5
42	205597.0
43	219409.0
44	233221.0
45	240472.0
46	247723.0
47	245447.5
48	243172.0
49	228119.0
50	213066.0
51	188065.0
52	163064.0
53	143589.0
54	124114.0
55	107545.0
56	90976.0
57	78350.0
58	65724.0
59	56971.0
60	48218.0
61	40913.0
62	33608.0
63	26950.5
64	20293.0
65	15743.0
66	11193.0
67	8958.0
68	6723.0
69	5248.0
70	3773.0
71	3097.0
72	2421.0
73	1815.0
74	1209.0
75	947.0
76	685.0
77	565.0
78	445.0
79	327.5
80	210.0
81	177.5
82	145.0
83	103.5
84	62.0
85	44.5
86	27.0
87	19.0
88	11.0
89	6.0
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2709620.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.31441453354641
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.41333697374677	26.220789208087158
2	7.168219576881067	4.919465160571592
3	3.207883992856482	3.3022998331921607
4	2.0055460147958377	2.752765492712255
5	1.4277226696098486	2.449573376196694
6	1.1003528547735135	2.2654778395121724
7	0.8999668932320434	2.161728592858257
8	0.7779874164948745	2.135694616919036
9	0.6438381109777868	1.9883635049328507
>10	5.974560346106391	40.70647754712509
>50	0.3500268661133936	7.681393699664596
>100	0.028621941429223082	1.6400275452688204
>500	0.0013668303408194034	0.3412900956556319
>1k	3.4170758520484797E-4	0.21316951233460887
>5k	0.0	0.0
>10k+	2.27805056803232E-4	1.2214839749690496
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19294	0.7120555649869724	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11958	0.44131649456381333	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3130	0.11551435256604246	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05288564448151401	0.0
2	0.0	0.0	0.0	0.16282725991098382	0.0
3	0.0	0.0	0.0	0.267196138203881	0.0
4	0.0	0.0	0.0	0.38603198972549657	0.0
5	0.0	0.0	0.0	0.628575224570235	0.0
6	0.0	0.0	0.0	1.0719953351392446	0.0
7	0.0	0.0	0.0	1.3867627194957226	0.0
8	0.0	0.0	0.0	2.1802688199821376	0.0
9	0.0	0.0	0.0	2.9612639410692276	0.0
10	0.0	0.0	0.0	3.9911131450166444	0.0
11	0.0	0.0	0.0	4.529528125715045	0.0
12	0.0	0.0	0.0	5.072593204951247	0.0
13	0.0	0.0	0.0	5.379868763885711	0.0
14	3.690554395081229E-5	0.0	0.0	5.516529993135569	0.0
15	3.690554395081229E-5	0.0	0.0	5.648947084831083	0.0
16	3.690554395081229E-5	0.0	0.0	5.8864342601545605	0.0
17	3.690554395081229E-5	0.0	0.0	6.141377757766771	0.0
18	3.690554395081229E-5	0.0	0.0	6.583469268753552	0.0
19	3.690554395081229E-5	0.0	0.0	6.765007639447598	0.0
20	3.690554395081229E-5	3.690554395081229E-5	0.0	6.992677940080159	0.0
21	3.690554395081229E-5	3.690554395081229E-5	0.0	7.199976380451871	0.0
22	3.690554395081229E-5	3.690554395081229E-5	0.0	7.415504757124615	0.0
23	3.690554395081229E-5	3.690554395081229E-5	0.0	7.675504314258088	0.0
24	3.690554395081229E-5	3.690554395081229E-5	0.0	7.86187731120969	0.0
25	3.690554395081229E-5	3.690554395081229E-5	0.0	8.033008318509607	0.0
26	3.690554395081229E-5	3.690554395081229E-5	0.0	8.194322451118607	0.0
27	3.690554395081229E-5	3.690554395081229E-5	0.0	8.37527033310944	0.0
28	3.690554395081229E-5	3.690554395081229E-5	0.0	8.554815804430142	0.0
29	3.690554395081229E-5	3.690554395081229E-5	0.0	8.743882906090153	0.0
30	3.690554395081229E-5	3.690554395081229E-5	0.0	8.99052265631343	0.0
31	3.690554395081229E-5	3.690554395081229E-5	0.0	9.201806895431831	0.0
32	3.690554395081229E-5	3.690554395081229E-5	0.0	9.403421882035119	0.0
33	3.690554395081229E-5	3.690554395081229E-5	0.0	9.599980809117145	0.0
34	3.690554395081229E-5	3.690554395081229E-5	0.0	9.790376510359387	0.0
35	3.690554395081229E-5	3.690554395081229E-5	0.0	10.003801271026934	0.0
36	3.690554395081229E-5	3.690554395081229E-5	0.0	10.201541175515386	0.0
37	3.690554395081229E-5	3.690554395081229E-5	0.0	10.415888574781704	0.0
38	7.381108790162458E-5	3.690554395081229E-5	0.0	10.63650991651966	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGAGT	50	2.7284841E-11	44.0	4
CGTTTTT	28280	0.0	43.25318	1
GAGCGAT	1785	0.0	41.535015	7
CGACGGT	325	0.0	40.615387	28
TCGCGCG	60	3.6379788E-12	40.333336	1
CGATCGA	50	1.3496901E-9	39.6	10
GTTTTTA	12760	0.0	39.517242	2
CGCGCGA	45	2.3530447E-8	39.11111	2
TCACGAC	335	0.0	38.74627	25
GCCGATT	80	0.0	38.500004	9
GTTAGCG	150	0.0	38.13333	1
GCGATCG	135	0.0	37.48148	9
GCGCGAC	395	0.0	37.316456	9
TATCGCG	95	0.0	37.05263	1
TATACGA	125	0.0	36.96	2
AGACCGA	605	0.0	36.727272	8
GACCGAT	1440	0.0	36.51389	9
ACGAGAT	350	0.0	36.45714	5
CGTAAGA	145	0.0	36.41379	2
AGCGATC	225	0.0	36.17778	8
>>END_MODULE