FastQCFastQC Report
Thu 26 May 2016
SRR1546751_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546751_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3199513
Sequences flagged as poor quality0
Sequence length50
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT312610.9770549455495258No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190660.5959031890165785No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA551.8189894E-1244.031
CGTTTTT437900.043.5026251
CGACGGT2600.042.30769328
GAGCGAT20800.041.567317
GTTTTTA193500.040.134372
TATCGCG850.038.8235321
AGCGATA4450.038.56188
CACGACG2950.038.033926
AGCGATC2900.037.9310388
ACGTCGC1400.037.71428320
GACCGAT14400.037.5833329
GACGGTC2950.037.2881429
AATCGCG651.0913936E-1137.230771
TATTGCG2150.036.8372081
ACGAGAT5800.036.7931065
GAGACCG7050.036.510647
GCGCGAC2350.036.5106359
CGGTCTA2900.036.41379531
ATCGCGA850.036.2352942
GTTTTTT316900.036.1760832
Produced by FastQC (version 0.11.5)