##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546751_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3199513
Sequences flagged as poor quality	0
Sequence length	50
%GC	40
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.522125398459078	31.0	31.0	33.0	30.0	34.0
2	31.91901173709874	31.0	31.0	34.0	30.0	34.0
3	31.97321623634597	31.0	31.0	34.0	30.0	34.0
4	35.71498006102804	37.0	35.0	37.0	33.0	37.0
5	35.625142638895355	37.0	35.0	37.0	33.0	37.0
6	35.67534934222802	37.0	35.0	37.0	33.0	37.0
7	36.06088957913283	37.0	35.0	37.0	35.0	37.0
8	21.952547465817453	35.0	0.0	37.0	0.0	37.0
9	29.891223133020556	37.0	18.0	39.0	17.0	39.0
10	34.358707403282935	35.0	32.0	39.0	27.0	39.0
11	36.01423747926638	37.0	35.0	39.0	32.0	39.0
12	36.71217244624416	39.0	35.0	39.0	33.0	39.0
13	36.949888623674916	39.0	37.0	39.0	33.0	39.0
14	38.177068510113884	40.0	37.0	41.0	33.0	41.0
15	38.362616123141244	40.0	38.0	41.0	33.0	41.0
16	38.35622014975404	40.0	37.0	41.0	33.0	41.0
17	38.29534651054708	40.0	37.0	41.0	33.0	41.0
18	38.160365968195784	40.0	37.0	41.0	33.0	41.0
19	38.087681469023565	40.0	37.0	41.0	34.0	41.0
20	37.90686551359535	40.0	36.0	41.0	33.0	41.0
21	37.84588873369166	40.0	36.0	41.0	33.0	41.0
22	37.81760067860328	40.0	36.0	41.0	33.0	41.0
23	37.61854757270872	39.0	35.0	41.0	33.0	41.0
24	37.37595502815585	39.0	35.0	41.0	33.0	41.0
25	37.24713854889791	39.0	35.0	41.0	33.0	41.0
26	37.18423960146435	39.0	35.0	41.0	33.0	41.0
27	37.07722237728054	39.0	35.0	41.0	32.0	41.0
28	37.03959040016402	39.0	35.0	41.0	32.0	41.0
29	36.92180903781294	39.0	35.0	41.0	32.0	41.0
30	36.74569067229919	39.0	35.0	41.0	31.0	41.0
31	36.513275926680095	39.0	35.0	41.0	31.0	41.0
32	35.98358312655707	39.0	35.0	41.0	28.0	41.0
33	35.50499591656605	39.0	35.0	41.0	24.0	41.0
34	35.08476915080514	39.0	35.0	41.0	21.0	41.0
35	34.724772488813144	39.0	35.0	41.0	18.0	41.0
36	34.556488753132115	39.0	34.0	41.0	18.0	41.0
37	34.515715360431415	39.0	34.0	41.0	17.0	41.0
38	34.42406016165585	39.0	34.0	41.0	16.0	41.0
39	34.30850695090159	39.0	34.0	41.0	15.0	41.0
40	34.26819956662155	39.0	34.0	40.0	15.0	41.0
41	34.23682729215353	39.0	34.0	40.0	15.0	41.0
42	33.99784967274707	38.0	33.0	40.0	15.0	41.0
43	34.03247650501811	38.0	33.0	40.0	15.0	41.0
44	33.81940532824839	38.0	33.0	40.0	12.0	41.0
45	33.65701217654062	38.0	33.0	40.0	11.0	41.0
46	33.72485625156079	38.0	33.0	40.0	12.0	41.0
47	33.62010093411091	38.0	33.0	40.0	11.0	41.0
48	33.60200224221624	38.0	33.0	40.0	11.0	41.0
49	33.51762127548786	38.0	33.0	40.0	10.0	41.0
50	33.09862063382771	37.0	32.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	2.0
13	5.0
14	13.0
15	44.0
16	139.0
17	451.0
18	1188.0
19	2872.0
20	5619.0
21	9962.0
22	15999.0
23	24325.0
24	38539.0
25	61695.0
26	85534.0
27	91441.0
28	81772.0
29	74267.0
30	75536.0
31	87666.0
32	107995.0
33	137320.0
34	192237.0
35	271618.0
36	290960.0
37	433849.0
38	525920.0
39	582544.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.51336656547418	21.588535505247204	26.57391921833104	22.324178710947574
2	38.105705462050004	22.922332242438145	27.10890688676683	11.86305540874502
3	25.158797604510436	22.69432879316321	40.1357644116464	12.011109190679957
4	22.310989203669436	25.11379075503053	39.67169378589804	12.903526255401996
5	20.202011993700292	29.074643547314853	38.33570921574627	12.38763524323858
6	17.89044145155841	37.18584672104786	35.18347948578424	9.74023234160949
7	79.19305219263056	2.728759033015337	16.443502495536038	1.6346862788180576
8	48.796144913304	39.95361169027911	9.636560314022791	1.6136830823941017
9	73.885150646364	4.984946146491669	18.256153358339223	2.873749848805115
10	31.896291716895664	33.20961658852457	24.07441382485397	10.8196778697258
11	22.126836177880822	24.52704520969285	38.76721238513486	14.578906227291466
12	21.531620593509075	22.132743326875058	39.135643455738425	17.199992623877446
13	19.009080444430136	24.893382211605328	40.39796056462343	15.699576779341108
14	16.954517765672463	27.5667890707117	38.31980054464539	17.15889261897045
15	16.125485347301293	24.478287789422954	43.54547082634138	15.85075603693437
16	18.03286937730836	24.117607898452047	41.7010651308496	16.148457593389995
17	18.519318408770335	23.725829524680787	38.17984174466552	19.57501032188336
18	19.417330074920777	23.63459689021423	39.71038717454813	17.23768586031687
19	20.671927258929717	25.7711720502464	36.63851342376168	16.918387267062208
20	21.932025280097314	24.247315138272608	36.21873078809181	17.601928793538267
21	20.572443368725178	25.793269163150768	36.99041072813269	16.643876739991367
22	19.81826609237093	23.526049120600543	36.75499990154752	19.90068488548101
23	17.557734567729526	26.930504736189537	36.59578817151235	18.915972524568584
24	17.231622437539713	24.924199401596432	39.58321157001081	18.260966590853045
25	17.371081161414253	26.39945516708324	38.08854660068579	18.140917070816716
26	17.464939195433804	27.367790035546037	37.31111578543359	17.856154983586563
27	17.92585309076725	26.228366629546436	37.505864173703934	18.33991610598238
28	17.361517205899773	26.126944944433735	39.51182570597463	16.999712143691866
29	18.177672664558635	24.345736366753314	38.44710116820904	19.02948980047901
30	18.872184610595426	25.54138707984621	37.376625755232126	18.209802554326238
31	20.431765709343892	25.42411923314579	35.1232203150917	19.020894742418612
32	21.062736735246894	25.550982290117275	35.52324994460094	17.863031030034886
33	20.53946960052983	27.20439016812871	33.83786845060482	18.41827178073663
34	19.714812848080317	27.186512447363082	33.81323970241721	19.28543500213939
35	20.205793819246868	28.095994609179588	32.52298084114676	19.175230730426787
36	22.136900209500634	27.636018356543634	31.070728576505235	19.156352857450493
37	20.583976373904402	29.956277721015667	31.06654043912308	18.39320546595685
38	20.79782141844712	29.683173657991073	30.37184096454679	19.14716395901501
39	20.5695991858761	29.93933764294754	30.22472482530935	19.266338345867013
40	21.540246906326058	27.973475963373172	31.224158176572498	19.262118953728272
41	19.09606243200137	28.015919922813254	31.061914735148754	21.82610291003662
42	20.641203833208365	28.23379683095521	30.63694380988607	20.488055525950355
43	20.44679924726044	28.061895669747237	31.336487771732763	20.154817311259556
44	20.635671741293127	29.20019390450984	30.913329622351903	19.25080473184513
45	19.40695349573513	29.25288942410923	31.143239611778416	20.19691746837722
46	20.76672293564677	28.823824125734138	30.47057474059333	19.938878198025762
47	20.271491317584893	27.754130081671804	31.23562867223856	20.738749928504742
48	20.68636695647119	26.802203960415227	31.461850600388246	21.04957848272534
49	20.58260116461474	26.488156166266553	31.558927874335872	21.37031479478283
50	19.706874139908166	27.154070010029653	32.34701656158296	20.792039288479216
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1226.0
1	2127.5
2	3029.0
3	47175.0
4	91321.0
5	67950.5
6	44580.0
7	44819.0
8	45058.0
9	45974.0
10	46890.0
11	46533.0
12	46176.0
13	44377.5
14	42579.0
15	40458.0
16	38337.0
17	36175.0
18	34013.0
19	32445.0
20	30877.0
21	30269.0
22	29661.0
23	31134.5
24	32608.0
25	36110.0
26	39612.0
27	45058.5
28	50505.0
29	58305.5
30	66106.0
31	76561.5
32	87017.0
33	97162.5
34	107308.0
35	116637.5
36	125967.0
37	136364.5
38	146762.0
39	162496.5
40	178231.0
41	196043.0
42	213855.0
43	227913.5
44	241972.0
45	254334.0
46	266696.0
47	269238.0
48	271780.0
49	258039.5
50	244299.0
51	215805.5
52	187312.0
53	164279.0
54	141246.0
55	123970.0
56	106694.0
57	93865.0
58	81036.0
59	70712.0
60	60388.0
61	50643.0
62	40898.0
63	32792.5
64	24687.0
65	19046.0
66	13405.0
67	10309.0
68	7213.0
69	5771.0
70	4329.0
71	3537.0
72	2745.0
73	2071.0
74	1397.0
75	1101.0
76	805.0
77	624.5
78	444.0
79	345.0
80	246.0
81	186.0
82	126.0
83	87.5
84	49.0
85	32.0
86	15.0
87	12.0
88	9.0
89	6.0
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3199513.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.99616390347953
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.60401440562852	28.70274209715958
2	7.833302814330301	6.109375209064237
3	3.877125359863779	4.53579048022755
4	2.74303971657558	4.278721055253415
5	1.931893804097451	3.7668223714350386
6	1.5403244335205817	3.6040046444461717
7	1.2058423038099608	3.291625688478581
8	1.0331571419746393	3.2231332197194797
9	0.8399322129883773	2.9478720820956332
>10	5.3217851705073445	34.59755278425895
>50	0.052357799403047384	1.3196887160297923
>100	0.0144962015381139	1.014343645380648
>500	0.0017604101691902332	0.46441213223754363
>1k	7.921845760844646E-4	0.4164606356361054
>5k	0.0	0.0
>10k+	1.7604101690765878E-4	1.7274552385772917
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31261	0.9770549455495258	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19066	0.5959031890165785	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0538832003495532	0.0
2	0.0	0.0	0.0	0.16830686420089558	0.0
3	0.0	0.0	0.0	0.2953261949552948	0.0
4	0.0	0.0	0.0	0.38387092035569165	0.0
5	0.0	0.0	0.0	0.5777129206851168	0.0
6	0.0	0.0	0.0	1.013654265508532	0.0
7	0.0	0.0	0.0	1.3509243437985718	0.0
8	3.1254756583267515E-5	0.0	0.0	2.1975844448827058	0.0
9	3.1254756583267515E-5	0.0	0.0	3.0495265998294117	0.0
10	3.1254756583267515E-5	0.0	0.0	4.125096538129396	0.0
11	3.1254756583267515E-5	0.0	0.0	4.636768158154069	0.0
12	3.1254756583267515E-5	0.0	0.0	5.179788299031759	0.0
13	3.1254756583267515E-5	0.0	0.0	5.535404919436177	0.0
14	3.1254756583267515E-5	0.0	0.0	5.724808744330778	0.0
15	3.1254756583267515E-5	0.0	0.0	5.871049750383887	0.0
16	3.1254756583267515E-5	0.0	0.0	6.076362246379371	0.0
17	3.1254756583267515E-5	0.0	0.0	6.287050560507177	0.0
18	3.1254756583267515E-5	0.0	0.0	6.680266653081266	0.0
19	3.1254756583267515E-5	0.0	0.0	6.845260513084335	0.0
20	3.1254756583267515E-5	0.0	0.0	7.040383958433674	0.0
21	3.1254756583267515E-5	0.0	0.0	7.20859705836482	0.0
22	3.1254756583267515E-5	0.0	0.0	7.3919999699954335	0.0
23	3.1254756583267515E-5	0.0	0.0	7.6019381699652415	0.0
24	3.1254756583267515E-5	3.1254756583267515E-5	0.0	7.769963741356888	0.0
25	3.1254756583267515E-5	3.1254756583267515E-5	0.0	7.926300033786392	0.0
26	3.1254756583267515E-5	3.1254756583267515E-5	0.0	8.080354728985318	0.0
27	3.1254756583267515E-5	3.1254756583267515E-5	0.0	8.27866615950615	0.0
28	3.1254756583267515E-5	3.1254756583267515E-5	0.0	8.444316369397468	0.0
29	3.1254756583267515E-5	3.1254756583267515E-5	0.0	8.61765524940827	0.0
30	3.1254756583267515E-5	3.1254756583267515E-5	0.0	8.849971855091697	0.0
31	3.1254756583267515E-5	2.8129280924940765E-4	0.0	9.031812028893148	0.0
32	3.1254756583267515E-5	2.8129280924940765E-4	0.0	9.21646513078709	0.0
33	6.250951316653503E-5	3.1254756583267514E-4	0.0	9.396586292976462	0.0
34	9.376426974980255E-5	3.1254756583267514E-4	0.0	9.577457569323832	0.0
35	9.376426974980255E-5	3.1254756583267514E-4	0.0	9.762985804402108	0.0
36	9.376426974980255E-5	3.1254756583267514E-4	0.0	9.943325749887562	0.0
37	9.376426974980255E-5	3.1254756583267514E-4	0.0	10.138886761829065	0.0
38	9.376426974980255E-5	3.1254756583267514E-4	0.0	10.341448839245222	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTAA	55	1.8189894E-12	44.0	31
CGTTTTT	43790	0.0	43.502625	1
CGACGGT	260	0.0	42.307693	28
GAGCGAT	2080	0.0	41.56731	7
GTTTTTA	19350	0.0	40.13437	2
TATCGCG	85	0.0	38.823532	1
AGCGATA	445	0.0	38.5618	8
CACGACG	295	0.0	38.0339	26
AGCGATC	290	0.0	37.931038	8
ACGTCGC	140	0.0	37.714283	20
GACCGAT	1440	0.0	37.583332	9
GACGGTC	295	0.0	37.28814	29
AATCGCG	65	1.0913936E-11	37.23077	1
TATTGCG	215	0.0	36.837208	1
ACGAGAT	580	0.0	36.793106	5
GAGACCG	705	0.0	36.51064	7
GCGCGAC	235	0.0	36.510635	9
CGGTCTA	290	0.0	36.413795	31
ATCGCGA	85	0.0	36.235294	2
GTTTTTT	31690	0.0	36.176083	2
>>END_MODULE