FastQCFastQC Report
Thu 26 May 2016
SRR1546750_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546750_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1967994
Sequences flagged as poor quality0
Sequence length50
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88200.44817209808566494No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54270.27576303586291423No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA35690.18135217891924466No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTAT551.8189894E-1244.042
TATCGCG408.3182385E-944.01
TCGAACG207.857851E-444.014
CGTTTTT97200.043.0041161
CGTAAGA1300.040.6153872
GAGCGAT14650.040.2457357
GCGTAAG1300.038.923081
TCGTTAG850.038.823531
GTCGTAA357.2928506E-637.71428742
GTTTTTA46150.037.5644652
CGAGTAC950.037.052636
TCGCTAT950.037.0526323
TATAGCG950.037.052631
CGACGGT2450.036.81632628
CGTTAGA900.036.6666682
CCGTATC301.3014325E-436.66666444
TATTACG601.9826984E-1036.6666641
CATACCG301.3014325E-436.6666641
AGACCGA4100.036.4878048
GCGATAT2050.036.4878049
Produced by FastQC (version 0.11.5)