##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546750_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1967994
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.492634123884525	31.0	31.0	33.0	30.0	34.0
2	31.8966521239394	31.0	31.0	34.0	30.0	34.0
3	32.03036899502742	33.0	31.0	34.0	30.0	34.0
4	35.7241688744986	37.0	35.0	37.0	35.0	37.0
5	35.613629411471784	37.0	35.0	37.0	33.0	37.0
6	35.66696189114398	37.0	35.0	37.0	33.0	37.0
7	36.01091263489624	37.0	35.0	37.0	35.0	37.0
8	22.088383399542884	35.0	0.0	37.0	0.0	37.0
9	29.962080677075235	37.0	18.0	39.0	17.0	39.0
10	34.36673536606311	35.0	32.0	39.0	27.0	39.0
11	36.00679575242607	37.0	35.0	39.0	32.0	39.0
12	36.442359580364574	37.0	35.0	39.0	32.0	39.0
13	36.568775616185825	39.0	35.0	39.0	33.0	39.0
14	37.66322763179156	39.0	36.0	41.0	33.0	41.0
15	37.917862554459006	40.0	36.0	41.0	33.0	41.0
16	37.95569752753311	40.0	36.0	41.0	33.0	41.0
17	37.91038184059504	40.0	36.0	41.0	33.0	41.0
18	37.874689658606684	40.0	36.0	41.0	33.0	41.0
19	37.86740965673676	40.0	36.0	41.0	33.0	41.0
20	37.746407763438306	40.0	36.0	41.0	33.0	41.0
21	37.659755568360474	40.0	35.0	41.0	33.0	41.0
22	37.61568632831197	39.0	35.0	41.0	33.0	41.0
23	37.48738055095696	39.0	35.0	41.0	33.0	41.0
24	37.36034865959957	39.0	35.0	41.0	33.0	41.0
25	37.21319069062202	39.0	35.0	41.0	32.0	41.0
26	37.16105943412429	39.0	35.0	41.0	32.0	41.0
27	37.046636829177324	39.0	35.0	41.0	32.0	41.0
28	37.06366127132502	39.0	35.0	41.0	32.0	41.0
29	36.944062837589954	39.0	35.0	41.0	32.0	41.0
30	36.84317330235763	39.0	35.0	41.0	31.0	41.0
31	36.73874615471389	39.0	35.0	41.0	31.0	41.0
32	36.498005583350356	39.0	35.0	41.0	30.0	41.0
33	36.33490193567663	39.0	35.0	41.0	30.0	41.0
34	36.18469619317945	39.0	35.0	41.0	30.0	41.0
35	36.00232978352576	39.0	35.0	41.0	29.0	41.0
36	35.85747466709756	39.0	35.0	41.0	29.0	41.0
37	35.81709090576496	39.0	35.0	41.0	28.0	41.0
38	35.71271812820567	38.0	35.0	40.0	28.0	41.0
39	35.62417669972571	38.0	35.0	40.0	27.0	41.0
40	35.53449858078836	38.0	35.0	40.0	27.0	41.0
41	35.50586993659533	38.0	35.0	40.0	27.0	41.0
42	35.27467309351553	38.0	35.0	40.0	26.0	41.0
43	35.3070248181651	38.0	35.0	40.0	26.0	41.0
44	35.08178734284759	38.0	34.0	40.0	26.0	41.0
45	34.889482894764924	38.0	34.0	40.0	24.0	41.0
46	34.95484081760412	38.0	34.0	40.0	25.0	41.0
47	34.8237260885958	37.0	34.0	40.0	24.0	41.0
48	34.822154437462714	37.0	34.0	40.0	25.0	41.0
49	34.73660641241793	37.0	34.0	40.0	24.0	41.0
50	34.28723715621084	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	2.0
12	1.0
13	10.0
14	9.0
15	44.0
16	96.0
17	245.0
18	633.0
19	1323.0
20	2452.0
21	4134.0
22	6875.0
23	9923.0
24	14454.0
25	20905.0
26	28383.0
27	33259.0
28	35324.0
29	38841.0
30	45887.0
31	57238.0
32	73345.0
33	97142.0
34	148160.0
35	225430.0
36	196448.0
37	284947.0
38	329706.0
39	312775.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.020016321187974	24.210846171278977	28.867770938326032	14.90136656920702
2	32.00639839349104	25.721572321866837	28.946023209420353	13.326006075221775
3	28.435452547111424	25.151347006139247	33.22250982472508	13.190690622024254
4	24.889608403277652	27.31339628067972	32.709906635894214	15.087088680148414
5	23.063739015464478	31.33911993634127	31.288713278597395	14.30842776959686
6	20.553670387206466	39.882946797602024	28.85440707644434	10.70897573874717
7	85.90371718612964	4.147217928509945	8.140573599309754	1.8084912860506688
8	53.608801652850566	39.984674750024645	4.761650696089521	1.6448729010352672
9	81.71564547452888	5.166326726605874	9.911107452563371	3.206920346301869
10	43.36059967662503	27.46873212011825	15.930739626238699	13.23992857701802
11	35.93923558710036	23.972430810256533	24.855055452404834	15.233278150238263
12	32.28719193249573	21.68482220982381	28.568532221134816	17.45945363654564
13	23.41003072163838	30.077683163668183	29.20506871464039	17.307217400053048
14	18.753715712547905	33.05284467330693	30.5463329664623	17.647106647682868
15	17.602543503689542	23.063789828627527	41.36857124564404	17.96509542203889
16	19.939694938094323	21.120084715705435	39.076237021047824	19.863983325152414
17	19.97307918621703	21.443002366877135	30.296128951612655	28.287789495293175
18	22.883098220827907	23.065822355149457	33.0743894544394	20.97668996958324
19	27.352115910922493	24.828734233945834	27.544240480407968	20.274909374723705
20	28.967974495857202	24.03894524068671	26.633109653789592	20.35997060966649
21	24.482391714608887	26.519948739681116	28.821530959952113	20.176128585757883
22	23.32669713423923	23.663080273618718	28.262484540095144	24.747738052046905
23	21.53284003914646	27.30308120858092	27.358772435281814	23.805306316990805
24	21.513988355655556	24.71689446207661	33.75269436797063	20.016422814297197
25	21.10240173496464	24.626751910828997	32.00472155911044	22.26612479509592
26	19.686035628157402	29.274530308527364	28.48789173137723	22.551542331938006
27	20.379838556418363	28.58575788340818	29.348056955458198	21.686346604715258
28	19.502091977922696	26.522895903137915	33.97200397968693	20.003008139252458
29	20.08969539541279	25.4772626339308	32.97510053384309	21.457941436813325
30	21.88507688539701	27.33763415945374	30.57021515309498	20.207073802054275
31	26.361055978829206	25.596622753931157	26.792764612087232	21.249556655152404
32	27.358467556303523	26.188087971812923	26.790376393423966	19.663068078459588
33	26.7338721561143	26.476960803742287	25.894946834187504	20.894220205955914
34	21.803725011356743	27.424626294592365	28.617262044498105	22.154386649552794
35	22.801746346787645	26.39205200828864	28.779914979415587	22.026286665508128
36	27.49083584604425	25.94967261079048	26.429806188433503	20.129685354731773
37	23.29976615782365	29.244753794981083	27.076505314548722	20.378974732646544
38	23.652358696215536	29.4954659414612	25.097485053308088	21.75469030901517
39	23.083048017422815	28.23214908175533	26.09840273903274	22.58640016178911
40	25.065117068446348	25.175991390217657	26.84505135686389	22.913840184472107
41	20.939647173721056	25.03467998378044	27.390022530556497	26.635650311942012
42	23.087976894238498	26.729959542559584	25.651602596349377	24.53046096685254
43	22.78157352105748	26.078077473813437	27.15867019919776	23.98167880593132
44	22.22582995679865	27.68626327112786	28.248155228115536	21.83975154395796
45	20.964291557799463	29.611828084841722	26.34576121675168	23.078119140607136
46	23.167651933898174	28.05567496648872	27.058314202177446	21.718358897435664
47	22.241988542647995	27.245916400151625	27.537990461353033	22.974104595847344
48	23.237824912067822	25.149924237573895	28.974326141238237	22.637924709120046
49	22.798189425374265	24.227716141411	29.689775476957752	23.284318956256982
50	21.486904939750833	27.913398109953587	27.872493513699737	22.727203436595843
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	985.0
1	1590.5
2	2196.0
3	13111.0
4	24026.0
5	17088.5
6	10151.0
7	10581.0
8	11011.0
9	11513.5
10	12016.0
11	12275.5
12	12535.0
13	12044.0
14	11553.0
15	11004.5
16	10456.0
17	10038.0
18	9620.0
19	9553.0
20	9486.0
21	9841.0
22	10196.0
23	10694.5
24	11193.0
25	12757.0
26	14321.0
27	16791.0
28	19261.0
29	22952.0
30	26643.0
31	30619.5
32	34596.0
33	39187.5
34	43779.0
35	48291.0
36	52803.0
37	60138.5
38	67474.0
39	83030.0
40	98586.0
41	115662.5
42	132739.0
43	142043.5
44	151348.0
45	158175.5
46	165003.0
47	165909.5
48	166816.0
49	161546.0
50	156276.0
51	141509.0
52	126742.0
53	115792.0
54	104842.0
55	100821.0
56	96800.0
57	93485.0
58	90170.0
59	84777.0
60	79384.0
61	71555.5
62	63727.0
63	55121.5
64	46516.0
65	39566.5
66	32617.0
67	27524.5
68	22432.0
69	18975.0
70	15518.0
71	12946.5
72	10375.0
73	8313.0
74	6251.0
75	4998.0
76	3745.0
77	2844.5
78	1944.0
79	1461.0
80	978.0
81	746.0
82	514.0
83	347.5
84	181.0
85	141.5
86	102.0
87	78.0
88	54.0
89	39.5
90	25.0
91	15.5
92	6.0
93	3.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1967994.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.644479984000192
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.02046897530396	21.942983109447052
2	6.734009507364121	3.9925242010624538
3	2.9614175841746397	2.63369052894994
4	1.9800388325687024	2.34788886158504
5	1.545361406824771	2.2905717646331656
6	1.2673439253484255	2.2541850976701383
7	1.0847078740709888	2.2508920602969384
8	0.9150136049806646	2.170008199834974
9	0.8292537038192928	2.2124515342076143
>10	8.321196527151889	49.47183756676573
>50	0.3074431920041839	5.605181963228785
>100	0.03143606563260679	1.5545836876322925
>500	0.0015984005231292665	0.28088035399655825
>1k	3.552001162509481E-4	0.24223627155354538
>5k	3.552001162509481E-4	0.7500847991357011
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8820	0.44817209808566494	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5427	0.27576303586291423	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3569	0.18135217891924466	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.055691226700894415	0.0
2	0.0	0.0	0.0	0.17083385416825458	0.0
3	0.0	0.0	0.0	0.2682935008948198	0.0
4	0.0	0.0	0.0	0.37373081421996207	0.0
5	0.0	0.0	0.0	0.584097309239764	0.0
6	0.0	0.0	0.0	0.8943624828124476	0.0
7	0.0	0.0	0.0	1.090043973711302	0.0
8	0.0	0.0	0.0	1.5847101159861259	0.0
9	0.0	0.0	0.0	2.052750160823661	0.0
10	0.0	0.0	0.0	2.782732061174983	0.0
11	0.0	0.0	0.0	3.2773982034498075	0.0
12	0.0	0.0	0.0	3.7140357135235167	0.0
13	0.0	0.0	0.0	3.9380201362402527	0.0
14	0.0	0.0	0.0	4.043813141706733	0.0
15	0.0	0.0	0.0	4.1588033296849485	0.0
16	0.0	0.0	0.0	4.367848682465495	0.0
17	0.0	0.0	0.0	4.6025546825854144	0.0
18	0.0	0.0	0.0	4.979283473425224	0.0
19	0.0	0.0	0.0	5.1386335527445715	0.0
20	0.0	0.0	0.0	5.3295386063168895	0.0
21	0.0	0.0	0.0	5.516327793682298	0.0
22	0.0	0.0	0.0	5.705606826037071	0.0
23	0.0	0.0	0.0	5.919479429307203	0.0
24	0.0	0.0	0.0	6.083148627485653	0.0
25	0.0	0.0	0.0	6.223494583824951	0.0
26	0.0	0.0	0.0	6.358555971207229	0.0
27	0.0	0.0	0.0	6.517143853080853	0.0
28	0.0	0.0	0.0	6.660945104507433	0.0
29	0.0	0.0	0.0	6.823140720957483	0.0
30	0.0	0.0	0.0	7.015112850953814	0.0
31	0.0	0.0	0.0	7.185082881350248	0.0
32	5.0813163048261324E-5	0.0	0.0	7.346871992495912	0.0
33	5.0813163048261324E-5	0.0	0.0	7.500530997553854	0.0
34	5.0813163048261324E-5	0.0	0.0	7.664860766851931	0.0
35	5.0813163048261324E-5	0.0	0.0	7.8471783958690935	0.0
36	5.0813163048261324E-5	0.0	0.0	8.020298842374519	0.0
37	5.0813163048261324E-5	0.0	0.0	8.200990450174137	0.0
38	1.0162632609652265E-4	0.0	0.0	8.388541834985269	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGTAT	55	1.8189894E-12	44.0	42
TATCGCG	40	8.3182385E-9	44.0	1
TCGAACG	20	7.857851E-4	44.0	14
CGTTTTT	9720	0.0	43.004116	1
CGTAAGA	130	0.0	40.615387	2
GAGCGAT	1465	0.0	40.245735	7
GCGTAAG	130	0.0	38.92308	1
TCGTTAG	85	0.0	38.82353	1
GTCGTAA	35	7.2928506E-6	37.714287	42
GTTTTTA	4615	0.0	37.564465	2
CGAGTAC	95	0.0	37.05263	6
TCGCTAT	95	0.0	37.05263	23
TATAGCG	95	0.0	37.05263	1
CGACGGT	245	0.0	36.816326	28
CGTTAGA	90	0.0	36.666668	2
CCGTATC	30	1.3014325E-4	36.666664	44
TATTACG	60	1.9826984E-10	36.666664	1
CATACCG	30	1.3014325E-4	36.666664	1
AGACCGA	410	0.0	36.487804	8
GCGATAT	205	0.0	36.487804	9
>>END_MODULE