FastQCFastQC Report
Thu 26 May 2016
SRR1546749_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546749_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2123403
Sequences flagged as poor quality0
Sequence length50
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175300.825561610301954No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107650.5069692375870242No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCGA207.857992E-444.02
CGCGATG254.44412E-544.01
CGTTTTT233350.043.406041
GCGATAC1150.042.086969
GAGCGAT13000.041.292317
GTTTTTA107900.039.7794232
CGGTCTA1850.039.2432431
TAAGCGA1350.039.111112
TAGGGCG6000.037.7666665
CTACGCG357.2931234E-637.7142871
CGACGGT1950.037.2307728
TGTTACG651.0913936E-1137.2307661
CGAGATT4900.036.8163266
CCGTCGC301.3014668E-436.66666421
CGGTCCG301.3014668E-436.66666429
GGTTCGC301.3014668E-436.6666649
CGGAGAT2000.036.35
GGACGTA553.426976E-936.08
GTTTTTT166150.035.8832362
AAGCGAC1350.035.85185215
Produced by FastQC (version 0.11.5)