##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546749_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2123403
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.49061482912099	31.0	31.0	33.0	30.0	34.0
2	31.889605505879008	31.0	31.0	34.0	30.0	34.0
3	31.965637234194357	31.0	31.0	34.0	30.0	34.0
4	35.69435618203421	37.0	35.0	37.0	33.0	37.0
5	35.59426637336389	37.0	35.0	37.0	33.0	37.0
6	35.64025481738511	37.0	35.0	37.0	33.0	37.0
7	36.04275165854056	37.0	35.0	37.0	35.0	37.0
8	21.947335008945547	35.0	0.0	37.0	0.0	37.0
9	29.87121521444587	37.0	18.0	39.0	17.0	39.0
10	34.36181261870686	35.0	32.0	39.0	27.0	39.0
11	35.945051410401135	37.0	35.0	39.0	32.0	39.0
12	36.562774470978894	38.0	35.0	39.0	32.0	39.0
13	36.76850932206463	39.0	35.0	39.0	33.0	39.0
14	37.95634460345022	40.0	37.0	41.0	33.0	41.0
15	38.173654270998014	40.0	37.0	41.0	33.0	41.0
16	38.17973884373338	40.0	37.0	41.0	33.0	41.0
17	38.12619460366214	40.0	37.0	41.0	33.0	41.0
18	38.01066683997338	40.0	37.0	41.0	33.0	41.0
19	37.96566972920355	40.0	37.0	41.0	33.0	41.0
20	37.80288433236649	40.0	36.0	41.0	33.0	41.0
21	37.72169672925959	40.0	35.0	41.0	33.0	41.0
22	37.691992994264396	39.0	35.0	41.0	33.0	41.0
23	37.51218492203317	39.0	35.0	41.0	33.0	41.0
24	37.31783839431328	39.0	35.0	41.0	33.0	41.0
25	37.17129390888117	39.0	35.0	41.0	32.0	41.0
26	37.10185866743148	39.0	35.0	41.0	32.0	41.0
27	36.98887493330282	39.0	35.0	41.0	32.0	41.0
28	36.95585812019669	39.0	35.0	41.0	32.0	41.0
29	36.8498118350591	39.0	35.0	41.0	31.0	41.0
30	36.70713190100984	39.0	35.0	41.0	31.0	41.0
31	36.533630686214536	39.0	35.0	41.0	31.0	41.0
32	36.0885319461261	39.0	35.0	41.0	29.0	41.0
33	35.717032989027516	39.0	35.0	41.0	26.0	41.0
34	35.41071902036495	39.0	35.0	41.0	24.0	41.0
35	35.12936027687632	39.0	35.0	41.0	23.0	41.0
36	34.985122466154564	39.0	35.0	41.0	21.0	41.0
37	34.960704115045516	39.0	35.0	41.0	21.0	41.0
38	34.85402347081548	39.0	34.0	41.0	21.0	41.0
39	34.749381535205515	39.0	34.0	41.0	19.0	41.0
40	34.70878584988342	39.0	34.0	40.0	19.0	41.0
41	34.6755547580935	38.0	34.0	40.0	18.0	41.0
42	34.42890727761051	38.0	34.0	40.0	18.0	41.0
43	34.4663589530579	38.0	34.0	40.0	18.0	41.0
44	34.252193295384814	38.0	34.0	40.0	18.0	41.0
45	34.0698647407016	38.0	33.0	40.0	17.0	41.0
46	34.14898443677436	38.0	34.0	40.0	18.0	41.0
47	34.041985906584856	38.0	33.0	40.0	18.0	41.0
48	34.0503408914841	38.0	33.0	40.0	18.0	41.0
49	33.96425596083268	38.0	33.0	40.0	17.0	41.0
50	33.529213248733285	37.0	33.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	0.0
11	1.0
12	2.0
13	1.0
14	12.0
15	32.0
16	109.0
17	329.0
18	917.0
19	2027.0
20	3746.0
21	6291.0
22	9850.0
23	14931.0
24	22577.0
25	34799.0
26	47950.0
27	53045.0
28	50241.0
29	47866.0
30	51662.0
31	60295.0
32	74199.0
33	95498.0
34	137690.0
35	198498.0
36	195361.0
37	291044.0
38	349375.0
39	375053.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.500947300159226	23.20303776532293	27.71028391690131	19.585731017616535
2	35.815292716455616	23.80151106502157	27.715464280685296	12.667731937837518
3	25.61289590341541	23.818088229130314	37.93740519345597	12.631610673998296
4	22.877098694877986	26.44260180474455	37.19680154921134	13.483497951166123
5	21.389910440929018	30.074366476829884	35.791604325697946	12.744118756543152
6	19.060347941488264	38.27907373211774	32.619290827035655	10.04128749935834
7	81.89133197984556	2.9665117737895255	13.475303557544189	1.6668526888207278
8	50.509959720316864	40.034275170563475	7.909473613817067	1.5462914953025875
9	76.71063853634944	5.326167477393598	15.183457873988122	2.7797361122688438
10	35.90185188586434	34.96331125085535	19.364058541878297	9.770778321402014
11	25.035144058852698	24.078236679518678	36.57426310502528	14.31235615660334
12	24.797506643816554	22.32915748918128	36.35061267220589	16.522723194796278
13	20.204878678234888	26.90944677011382	36.97776634958131	15.90790820206998
14	16.85172338929539	30.08053581915444	35.872606377592945	17.19513441395722
15	16.052487445859313	24.60908268472824	43.575242193780454	15.763187675631993
16	18.340088998649808	23.788277590264308	41.295316998233496	16.57631641285239
17	18.864624378886155	23.230729164459124	35.9571404957043	21.947505960950416
18	20.17054699461195	24.025444063138274	37.860123584642196	17.943885357607574
19	22.2719851106926	25.932854008400668	34.425872055375265	17.36928882553147
20	23.72983366793774	24.845213084845412	33.664358579129825	17.76059466808703
21	21.607532814072506	26.587887461777154	34.63666576716714	17.167913956983202
22	20.552339805491467	24.23053937476777	34.45525884629531	20.761861973445455
23	18.09562292226205	27.838474373446775	34.28020964461291	19.785693059678263
24	18.21114503464486	25.135596022045743	38.08805959113744	18.565199352171962
25	17.685479393219282	26.56118504118154	36.619661929459454	19.133673636139726
26	17.483727770941268	29.13290600041537	34.82075705836339	18.56260917027997
27	18.2564496706466	27.562784831706466	35.26829339508327	18.91247210256367
28	17.16315744114518	26.924375636654936	38.53149873104634	17.38096819115354
29	18.00548459242075	24.980467673823576	37.368883815272	19.64516391848368
30	19.16094118732996	26.280079664576157	35.249832462325806	19.30914668576808
31	21.561333387962627	26.157634702409293	32.38626864518888	19.894763264439206
32	22.31182681761305	27.06791880768747	32.02741071760754	18.592843657091944
33	21.362030664927946	27.80418978404005	31.382125766988178	19.45165378404382
34	18.93479476105101	28.288177044112683	32.2070751524793	20.56995304235701
35	19.82454578805813	28.048608766211597	31.98224736425445	20.14459808147582
36	22.179963012202585	28.119155902106197	30.340495892677932	19.36038519301329
37	20.715191605173395	29.446553480427408	31.11698532968071	18.721269584718492
38	20.65900820522529	29.630880242704755	29.886696025201058	19.8234155268689
39	20.457303677163495	29.6012108864874	30.30630549170365	19.635179944645458
40	21.972654272410843	27.230817701585615	30.317890668893284	20.47863735711026
41	18.787719523802124	27.40784486034917	30.76537991139694	23.03905570445177
42	20.88515463150424	28.071261084212463	29.29533395215134	21.74825033213196
43	20.71768759863295	28.20180625156883	29.91570606239136	21.164800087406867
44	20.11930848736674	29.80305669719785	30.21188158818651	19.865753227248902
45	19.412518490366644	29.902378399201655	29.77013784006145	20.91496527037025
46	20.865846002854852	29.465956297509234	29.647127747299972	20.021069952335942
47	20.56303019257296	27.2192796186122	30.90152929048325	21.316160898331592
48	21.12825497562168	26.11119980521832	31.113735828761662	21.646809390398335
49	20.479202487704878	25.82924673272101	31.551288191643316	22.14026258793079
50	19.373383196689463	28.041073691616713	31.416975486989518	21.168567624704306
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1011.0
1	1555.5
2	2100.0
3	26637.0
4	51174.0
5	37555.5
6	23937.0
7	24182.5
8	24428.0
9	24935.0
10	25442.0
11	25085.0
12	24728.0
13	23955.5
14	23183.0
15	22127.0
16	21071.0
17	19985.5
18	18900.0
19	18269.0
20	17638.0
21	17645.0
22	17652.0
23	18640.5
24	19629.0
25	22433.0
26	25237.0
27	29314.0
28	33391.0
29	38711.5
30	44032.0
31	49728.0
32	55424.0
33	61531.0
34	67638.0
35	74886.5
36	82135.0
37	90047.0
38	97959.0
39	109968.0
40	121977.0
41	135604.5
42	149232.0
43	159476.0
44	169720.0
45	176388.5
46	183057.0
47	182652.0
48	182247.0
49	172083.5
50	161920.0
51	142581.0
52	123242.0
53	109006.5
54	94771.0
55	84805.0
56	74839.0
57	67309.0
58	59779.0
59	53259.5
60	46740.0
61	39977.0
62	33214.0
63	26983.0
64	20752.0
65	15874.5
66	10997.0
67	8368.0
68	5739.0
69	4692.5
70	3646.0
71	2866.5
72	2087.0
73	1689.0
74	1291.0
75	1011.5
76	732.0
77	539.0
78	346.0
79	275.0
80	204.0
81	159.0
82	114.0
83	71.0
84	28.0
85	19.5
86	11.0
87	8.5
88	6.0
89	3.5
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2123403.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.96729115264393
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.127405840488	28.106096234712062
2	8.067656685519246	6.287494539684056
3	4.175779783610797	4.881564798518592
4	2.9709522774653303	4.6307984958640835
5	2.2264778022248572	4.337990438209739
6	1.7305228212826176	4.046027197392873
7	1.3680179041323766	3.7315566380649234
8	1.1370627113285914	3.5446602984924755
9	0.9396110953908733	3.295268921191577
>10	5.1977761503958035	33.31047527119046
>50	0.04584304200757967	1.147199688938277
>100	0.011234643978390446	0.8581104913560893
>500	0.001276340135011994	0.33830099106085876
>1k	1.276340135011993E-4	0.07718952339406829
>5k	0.0	0.0
>10k+	2.552680270023986E-4	1.4072664719298724
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17530	0.825561610301954	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10765	0.5069692375870242	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.050579188218157364	0.0
2	0.0	0.0	0.0	0.14961832492466104	0.0
3	0.0	0.0	0.0	0.2536965427664932	0.0
4	0.0	0.0	0.0	0.35052225131074977	0.0
5	0.0	0.0	0.0	0.5573129547240915	0.0
6	0.0	0.0	0.0	0.9527159940906178	0.0
7	0.0	0.0	0.0	1.2513875133453236	0.0
8	0.0	0.0	0.0	2.00206931986062	0.0
9	0.0	0.0	0.0	2.8131730057836406	0.0
10	0.0	0.0	0.0	3.836671606849948	0.0
11	0.0	0.0	0.0	4.345430424653257	0.0
12	0.0	0.0	0.0	4.853435734997078	0.0
13	0.0	0.0	0.0	5.174382818522909	0.0
14	0.0	0.0	0.0	5.330547239501875	0.0
15	0.0	0.0	0.0	5.459962145669004	0.0
16	0.0	0.0	0.0	5.680786925515316	0.0
17	0.0	0.0	0.0	5.918471434767682	0.0
18	0.0	0.0	0.0	6.343496736135345	0.0
19	0.0	0.0	0.0	6.5012623604657245	0.0
20	0.0	0.0	0.0	6.705839635716819	0.0
21	0.0	0.0	0.0	6.876838734804463	0.0
22	0.0	0.0	0.0	7.060741649135845	0.0
23	0.0	0.0	0.0	7.27054638238714	0.0
24	0.0	0.0	0.0	7.430242869582458	0.0
25	0.0	0.0	0.0	7.576847164669165	0.0
26	0.0	0.0	0.0	7.717423400079966	0.0
27	0.0	0.0	0.0	7.881876403113305	0.0
28	0.0	0.0	0.0	8.04185545560593	0.0
29	0.0	0.0	0.0	8.207909661990682	0.0
30	0.0	0.0	0.0	8.420069106052878	0.0
31	0.0	0.0	0.0	8.60157021535714	0.0
32	0.0	0.0	0.0	8.773982140931325	0.0
33	0.0	0.0	0.0	8.947383045046088	0.0
34	0.0	0.0	0.0	9.126011407161052	0.0
35	0.0	0.0	0.0	9.316224946465649	0.0
36	0.0	0.0	0.0	9.500457520310558	0.0
37	0.0	0.0	0.0	9.68746865291233	0.0
38	0.0	0.0	0.0	9.889220275190343	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCGA	20	7.857992E-4	44.0	2
CGCGATG	25	4.44412E-5	44.0	1
CGTTTTT	23335	0.0	43.40604	1
GCGATAC	115	0.0	42.08696	9
GAGCGAT	1300	0.0	41.29231	7
GTTTTTA	10790	0.0	39.779423	2
CGGTCTA	185	0.0	39.24324	31
TAAGCGA	135	0.0	39.11111	2
TAGGGCG	600	0.0	37.766666	5
CTACGCG	35	7.2931234E-6	37.714287	1
CGACGGT	195	0.0	37.23077	28
TGTTACG	65	1.0913936E-11	37.230766	1
CGAGATT	490	0.0	36.816326	6
CCGTCGC	30	1.3014668E-4	36.666664	21
CGGTCCG	30	1.3014668E-4	36.666664	29
GGTTCGC	30	1.3014668E-4	36.666664	9
CGGAGAT	200	0.0	36.3	5
GGACGTA	55	3.426976E-9	36.0	8
GTTTTTT	16615	0.0	35.883236	2
AAGCGAC	135	0.0	35.851852	15
>>END_MODULE