FastQCFastQC Report
Thu 26 May 2016
SRR1546748_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546748_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1229287
Sequences flagged as poor quality0
Sequence length50
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA37720.3068445367111179No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37290.30334657407098586No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24060.19572321191064415No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG302.5278605E-644.01
CGAACTA207.856696E-444.037
TCCGCGA302.5278605E-644.02
CGAGTCA207.856696E-444.06
GACCGTA207.856696E-444.09
TGTGCGA750.044.02
ACGGGTA800.044.05
TCGATAG254.4430202E-544.01
ACGATAT207.856696E-444.022
CCCGTAG207.856696E-444.035
CGCTCCA302.5278605E-644.015
CGAATAT408.312782E-944.014
GGACCGT254.4430202E-544.08
TCGGTCG207.856696E-444.015
TAGTCCG207.856696E-444.01
TCGAACG254.4430202E-544.01
TAATACG454.802132E-1043.9999961
TTGGCGA454.802132E-1043.9999962
CTAGCGA454.802132E-1043.9999962
TCACGAC3250.041.9692325
Produced by FastQC (version 0.11.5)