##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546748_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1229287
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.972983526222926	31.0	31.0	34.0	31.0	34.0
2	32.41645767017792	34.0	31.0	34.0	31.0	34.0
3	32.54460349779995	34.0	31.0	34.0	31.0	34.0
4	36.14015685515262	37.0	35.0	37.0	35.0	37.0
5	36.0748075917178	37.0	35.0	37.0	35.0	37.0
6	36.12392874894146	37.0	35.0	37.0	35.0	37.0
7	36.30237771976764	37.0	36.0	37.0	35.0	37.0
8	21.651977935177058	35.0	0.0	37.0	0.0	37.0
9	29.859185039783224	37.0	18.0	39.0	17.0	39.0
10	34.85505337646945	37.0	32.0	39.0	29.0	39.0
11	36.60702667481231	37.0	35.0	39.0	34.0	39.0
12	37.000764670902726	39.0	35.0	39.0	34.0	39.0
13	37.05929290718929	39.0	35.0	39.0	34.0	39.0
14	38.28761387698723	40.0	37.0	41.0	34.0	41.0
15	38.52369137556974	40.0	37.0	41.0	35.0	41.0
16	38.57996952705105	40.0	37.0	41.0	35.0	41.0
17	38.57898114923529	40.0	37.0	41.0	35.0	41.0
18	38.556379429701934	40.0	37.0	41.0	35.0	41.0
19	38.519754133900385	40.0	37.0	41.0	35.0	41.0
20	38.417878005705745	40.0	36.0	41.0	35.0	41.0
21	38.34635443147125	40.0	36.0	41.0	35.0	41.0
22	38.294558553047416	40.0	36.0	41.0	35.0	41.0
23	38.19193890442183	40.0	35.0	41.0	34.0	41.0
24	38.06247117231371	40.0	35.0	41.0	34.0	41.0
25	37.93884178389587	40.0	35.0	41.0	34.0	41.0
26	37.86860513452107	40.0	35.0	41.0	34.0	41.0
27	37.801453200107055	40.0	35.0	41.0	34.0	41.0
28	37.830226790001035	40.0	35.0	41.0	34.0	41.0
29	37.774903663668454	40.0	35.0	41.0	34.0	41.0
30	37.700763125291324	40.0	35.0	41.0	34.0	41.0
31	37.57224309701478	40.0	35.0	41.0	34.0	41.0
32	37.34147355336874	40.0	35.0	41.0	33.0	41.0
33	37.17069732291971	40.0	35.0	41.0	33.0	41.0
34	37.01563996039981	40.0	35.0	41.0	33.0	41.0
35	36.858544017792426	40.0	35.0	41.0	33.0	41.0
36	36.72770882633591	40.0	35.0	41.0	32.0	41.0
37	36.642429310649185	40.0	35.0	41.0	32.0	41.0
38	36.57040870032792	40.0	35.0	41.0	32.0	41.0
39	36.51925628433393	40.0	35.0	41.0	31.0	41.0
40	36.390164379839696	39.0	35.0	41.0	31.0	41.0
41	36.38083295438738	39.0	35.0	41.0	31.0	41.0
42	36.1598137782308	39.0	35.0	41.0	30.0	41.0
43	36.16408129265176	39.0	35.0	41.0	31.0	41.0
44	35.96339748162959	39.0	35.0	41.0	30.0	41.0
45	35.8010952690462	39.0	35.0	41.0	29.0	41.0
46	35.831057352758144	39.0	35.0	40.0	30.0	41.0
47	35.76191808747672	38.0	35.0	40.0	30.0	41.0
48	35.769862530068245	38.0	35.0	40.0	30.0	41.0
49	35.69496382862586	38.0	35.0	40.0	29.0	41.0
50	35.23539905652626	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	0.0
13	3.0
14	3.0
15	14.0
16	26.0
17	58.0
18	195.0
19	438.0
20	878.0
21	1642.0
22	2692.0
23	4470.0
24	7370.0
25	11013.0
26	14358.0
27	15617.0
28	14905.0
29	14477.0
30	15980.0
31	20106.0
32	27706.0
33	41369.0
34	79730.0
35	168796.0
36	109593.0
37	186606.0
38	249767.0
39	241473.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.030009265533597	25.239996843698826	31.656317849289874	13.073676041477702
2	31.028962317180607	26.319077644195378	29.409975050578097	13.241984988045916
3	27.166235386854332	25.48875893099008	33.60590325936904	13.739102422786543
4	24.148063064199	28.253288288251643	32.98082547037429	14.617823177175064
5	22.792968607005523	32.14050095705885	31.568787435318196	13.497743000617431
6	20.255562777447413	40.41139294566688	28.428674508068497	10.904369768817208
7	85.76207183513695	4.903330141781375	7.5098003964899975	1.8247976265916745
8	52.04634881846143	41.86882314707631	4.246526645120301	1.838301389341952
9	82.01550980365042	5.796530834540673	9.13952559491803	3.0484337668908887
10	46.486703267829235	26.583946629224908	14.849339495170778	12.080010607775076
11	40.74589579162555	22.72862236402077	23.06133555467519	13.464146289678489
12	35.569968607819	23.13137615544621	26.051605524177834	15.247049712556954
13	21.077095910068195	38.30106395007838	27.15330105988268	13.468539079970748
14	16.206467651573636	41.065593307339945	28.410859302994336	14.317079738092081
15	13.963948207375495	24.647051502212257	46.90597069683483	14.483029593577415
16	15.503133117001969	21.14746190271271	44.01396907312938	19.335435907155937
17	16.714160322203035	21.17170359728851	29.51491392978206	32.5992221507264
18	20.970041983686478	24.183856170284077	34.04607711624706	20.800024729782386
19	26.69466121418351	26.194289860707876	27.27206909371042	19.838979831398202
20	28.816216229407775	24.482809954062805	26.076823394374138	20.62415042215528
21	21.92710083162028	29.420631634435246	28.392067922299674	20.2601996116448
22	21.990877638826408	25.86889798720722	26.251721526380738	25.88850284758563
23	18.521793527467548	31.463441816272358	25.660647188166802	24.354117468093293
24	19.69938671766642	25.891838114289012	35.94791126889002	18.46086389915455
25	16.89873886244628	26.87167439336786	34.956198186428395	21.273388557757464
26	16.376566253446104	34.64056806913276	28.768302276034806	20.214563401386332
27	18.139620772041027	33.71352662152939	29.038458879008726	19.108393727420854
28	15.671848803412058	29.18569870176777	37.14974615366469	17.992706341155483
29	16.56480545226623	25.525853604569154	36.28306489859569	21.626276044568925
30	18.200550400354025	30.998944916850174	31.47906062620039	19.321444056595407
31	26.223493781354556	28.29428766431273	25.13278022138036	20.349438332952356
32	26.759332849041762	28.878854165056655	26.232116666002327	18.129696319899256
33	25.65511552631729	29.564617538459288	24.696429719015985	20.083837216207442
34	19.435249864352265	29.799794515031884	27.70752476842267	23.057430852193182
35	19.148742319734936	28.17519423861149	29.322770028479923	23.353293413173652
36	28.18877934933014	26.58427202109841	26.370408212240104	18.856540417331345
37	20.032994735972967	32.00155862707407	27.82214405586328	20.14330258108969
38	21.742684987313783	32.07281944737071	23.81486178573433	22.36963377958117
39	20.681175347986272	31.320350739900448	26.450454613121266	21.548019298992017
40	23.865053482221807	26.97685731647695	25.60980470793232	23.54828449336892
41	18.437110292389004	26.08032135701427	26.967827691987306	28.514740658609423
42	22.290319510415387	27.482190896023468	25.24837568444147	24.979113909119675
43	21.624405041296296	27.972963189230832	26.234150365211704	24.16848140426117
44	20.35114664028823	31.354760930523142	27.23033758593396	21.063754843254667
45	18.066977036282008	35.00874897399875	24.433187693353954	22.491086296365292
46	22.681115150489674	31.22671922830063	26.22023986261955	19.87192575859014
47	21.3771072174358	28.603572640075097	26.68839742061862	23.330922721870483
48	23.100626623400395	25.88484218900875	28.774810113504824	22.239721074086034
49	21.678176048392277	24.387876874968985	30.323105995589312	23.610841081049422
50	19.88965961569593	30.91824773222201	27.01850747628503	22.17358517579703
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1266.0
1	1481.0
2	1696.0
3	6086.5
4	10477.0
5	7937.0
6	5397.0
7	5982.5
8	6568.0
9	7334.0
10	8100.0
11	8487.5
12	8875.0
13	8739.5
14	8604.0
15	8243.0
16	7882.0
17	7763.5
18	7645.0
19	7462.5
20	7280.0
21	7087.5
22	6895.0
23	7215.5
24	7536.0
25	8090.5
26	8645.0
27	10089.0
28	11533.0
29	13871.0
30	16209.0
31	19531.0
32	22853.0
33	26023.0
34	29193.0
35	32651.5
36	36110.0
37	41464.0
38	46818.0
39	59757.0
40	72696.0
41	89732.5
42	106769.0
43	116606.0
44	126443.0
45	129364.5
46	132286.0
47	126414.5
48	120543.0
49	112457.5
50	104372.0
51	91251.0
52	78130.0
53	68767.0
54	59404.0
55	52880.0
56	46356.0
57	41440.0
58	36524.0
59	32418.5
60	28313.0
61	24747.5
62	21182.0
63	17411.5
64	13641.0
65	11129.0
66	8617.0
67	7001.5
68	5386.0
69	4628.5
70	3871.0
71	3150.0
72	2429.0
73	1840.0
74	1251.0
75	981.0
76	711.0
77	530.5
78	350.0
79	281.5
80	213.0
81	162.5
82	112.0
83	89.0
84	66.0
85	39.5
86	13.0
87	17.0
88	21.0
89	12.5
90	4.0
91	2.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1229287.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.24462865079763
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.4899850015404	23.27411919576615
2	7.513953859492234	4.695413960781249
3	3.265676356859353	3.0610453519128047
4	2.0078241505608725	2.5093487992151062
5	1.3996254386039746	2.1865388539695467
6	1.1358924566911073	2.129432279793352
7	0.9634327486026255	2.1071468922074414
8	0.8063658554086577	2.0155681367141023
9	0.6781742639747174	1.9070372734576242
>10	7.402370928831904	47.6094179834285
>50	0.29984603901462153	5.870155074164881
>100	0.035010255414939066	1.6026611250815646
>500	7.89705001937989E-4	0.13274633266725677
>1k	0.0010529400025839851	0.8993687408404293
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3772	0.3068445367111179	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3729	0.30334657407098586	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2406	0.19572321191064415	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04864608508834796	0.0
2	0.0	0.0	0.0	0.17205095311347146	0.0
3	0.0	0.0	0.0	0.27902353152681186	0.0
4	0.0	0.0	0.0	0.42496178679185576	0.0
5	0.0	0.0	0.0	0.7019516191092886	0.0
6	0.0	0.0	0.0	1.098848356811713	0.0
7	0.0	0.0	0.0	1.3465529205140867	0.0
8	0.0	0.0	0.0	1.9909915259821345	0.0
9	0.0	0.0	0.0	2.6467374990543298	0.0
10	0.0	0.0	0.0	3.693035068295687	0.0
11	0.0	0.0	0.0	4.447700170912081	0.0
12	0.0	0.0	0.0	5.086119026720367	0.0
13	0.0	0.0	0.0	5.39068582031698	0.0
14	0.0	0.0	0.0	5.52678097140863	0.0
15	0.0	0.0	0.0	5.6739394461993005	0.0
16	0.0	0.0	0.0	5.9841192496138005	0.0
17	0.0	0.0	0.0	6.336681344551761	0.0
18	0.0	0.0	0.0	6.932067125089585	0.0
19	0.0	0.0	0.0	7.170253976492064	0.0
20	0.0	0.0	0.0	7.472298983069047	0.0
21	0.0	0.0	0.0	7.7630366220418825	0.0
22	0.0	0.0	0.0	8.050764386184838	0.0
23	0.0	0.0	0.0	8.386731495574264	0.0
24	0.0	0.0	0.0	8.6155633306136	0.0
25	0.0	0.0	0.0	8.800304566793596	0.0
26	0.0	0.0	0.0	8.993261947779486	0.0
27	0.0	0.0	0.0	9.207939236321543	0.0
28	0.0	0.0	0.0	9.412122636943204	0.0
29	0.0	0.0	0.0	9.61069302774698	0.0
30	0.0	0.0	0.0	9.889879255210541	0.0
31	0.0	0.0	0.0	10.134248552209533	0.0
32	0.0	0.0	0.0	10.349657972466966	0.0
33	0.0	0.0	0.0	10.570517706605536	0.0
34	0.0	0.0	0.0	10.797153146498744	0.0
35	0.0	0.0	0.0	11.05876821279327	0.0
36	0.0	0.0	0.0	11.294026537334243	0.0
37	0.0	0.0	0.0	11.539290661985362	0.0
38	0.0	0.0	0.0	11.77430494262121	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACG	30	2.5278605E-6	44.0	1
CGAACTA	20	7.856696E-4	44.0	37
TCCGCGA	30	2.5278605E-6	44.0	2
CGAGTCA	20	7.856696E-4	44.0	6
GACCGTA	20	7.856696E-4	44.0	9
TGTGCGA	75	0.0	44.0	2
ACGGGTA	80	0.0	44.0	5
TCGATAG	25	4.4430202E-5	44.0	1
ACGATAT	20	7.856696E-4	44.0	22
CCCGTAG	20	7.856696E-4	44.0	35
CGCTCCA	30	2.5278605E-6	44.0	15
CGAATAT	40	8.312782E-9	44.0	14
GGACCGT	25	4.4430202E-5	44.0	8
TCGGTCG	20	7.856696E-4	44.0	15
TAGTCCG	20	7.856696E-4	44.0	1
TCGAACG	25	4.4430202E-5	44.0	1
TAATACG	45	4.802132E-10	43.999996	1
TTGGCGA	45	4.802132E-10	43.999996	2
CTAGCGA	45	4.802132E-10	43.999996	2
TCACGAC	325	0.0	41.96923	25
>>END_MODULE