FastQCFastQC Report
Thu 26 May 2016
SRR1546747_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546747_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3379952
Sequences flagged as poor quality0
Sequence length50
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122230.361632354542313No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75560.22355347058183073No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA56160.16615620576860263No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAA302.529181E-644.00000433
CGTTTTT168000.042.808331
TAATGCG1200.042.166671
AGCGATA4600.042.0869528
CGACGGT4650.041.6344128
CGGTCTA4550.041.58241331
TACGACG850.041.4117661
GAGCGAT21600.041.3518527
CGTAGGT700.040.85714312
CACGACG4700.040.72340826
TCACGAC4800.040.33333625
GTTTTTA78150.037.975692
CGTAAGA2900.037.9310382
ACGGTCT5050.037.9009930
TACGCGC1050.037.71428734
GACGGTC5200.036.38461329
ACGTGCG1150.036.3478241
ATGTCTC23650.036.2790688
TCGACGA850.036.2352942
GCGCGAC10550.035.86739
Produced by FastQC (version 0.11.5)