##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546747_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3379952
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.43834527827614	31.0	31.0	33.0	30.0	34.0
2	31.821637703730705	31.0	31.0	34.0	30.0	34.0
3	31.954686338740906	31.0	31.0	34.0	30.0	34.0
4	35.6642292553267	37.0	35.0	37.0	33.0	37.0
5	35.55234719309623	37.0	35.0	37.0	33.0	37.0
6	35.59462767518592	37.0	35.0	37.0	33.0	37.0
7	35.97800649239989	37.0	35.0	37.0	35.0	37.0
8	21.94235953646679	35.0	0.0	37.0	0.0	37.0
9	29.85154759594219	37.0	18.0	39.0	17.0	39.0
10	34.25554564088484	35.0	32.0	39.0	27.0	39.0
11	35.88224300226749	37.0	35.0	39.0	32.0	39.0
12	36.29900395035196	37.0	35.0	39.0	32.0	39.0
13	36.396676343332686	38.0	35.0	39.0	32.0	39.0
14	37.515086308917994	39.0	36.0	41.0	33.0	41.0
15	37.786787800536814	40.0	36.0	41.0	33.0	41.0
16	37.85344969396015	40.0	36.0	41.0	33.0	41.0
17	37.813249123064466	40.0	36.0	41.0	33.0	41.0
18	37.75982380814875	39.0	36.0	41.0	33.0	41.0
19	37.76972158184495	39.0	36.0	41.0	33.0	41.0
20	37.634145692009824	39.0	35.0	41.0	33.0	41.0
21	37.526069600988414	39.0	35.0	41.0	33.0	41.0
22	37.475650245920654	39.0	35.0	41.0	33.0	41.0
23	37.34469542762737	39.0	35.0	41.0	32.0	41.0
24	37.218441267804984	39.0	35.0	41.0	32.0	41.0
25	37.07603687863023	39.0	35.0	41.0	32.0	41.0
26	36.9884353387267	39.0	35.0	41.0	32.0	41.0
27	36.86721793682277	39.0	35.0	41.0	31.0	41.0
28	36.89705001727835	39.0	35.0	41.0	32.0	41.0
29	36.80258329112366	39.0	35.0	41.0	31.0	41.0
30	36.7199720587748	38.0	35.0	40.0	31.0	41.0
31	36.623872765057015	38.0	35.0	40.0	31.0	41.0
32	36.366915861527026	38.0	35.0	40.0	30.0	41.0
33	36.21751285225352	39.0	35.0	41.0	30.0	41.0
34	36.10275323436546	39.0	35.0	41.0	30.0	41.0
35	35.92483295620766	39.0	35.0	41.0	29.0	41.0
36	35.774878755674635	38.0	35.0	40.0	28.0	41.0
37	35.70324401056583	38.0	35.0	40.0	28.0	41.0
38	35.610792697647774	38.0	35.0	40.0	27.0	41.0
39	35.51858813379598	38.0	35.0	40.0	27.0	41.0
40	35.44955815940581	38.0	35.0	40.0	27.0	41.0
41	35.45469373529565	38.0	35.0	40.0	27.0	41.0
42	35.20726448186247	38.0	34.0	40.0	25.0	41.0
43	35.2505979374855	38.0	35.0	40.0	26.0	41.0
44	35.06335888793687	38.0	34.0	40.0	25.0	41.0
45	34.84699871477465	38.0	34.0	40.0	24.0	41.0
46	34.92539953230105	38.0	34.0	40.0	24.0	41.0
47	34.79044110685596	38.0	34.0	40.0	24.0	41.0
48	34.81333699413483	37.0	34.0	40.0	24.0	41.0
49	34.73268259430903	37.0	34.0	40.0	24.0	41.0
50	34.23790929575331	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	2.0
12	1.0
13	6.0
14	18.0
15	62.0
16	138.0
17	423.0
18	1050.0
19	2389.0
20	4571.0
21	7677.0
22	12294.0
23	18557.0
24	26661.0
25	38445.0
26	50456.0
27	57783.0
28	61253.0
29	68174.0
30	81846.0
31	102288.0
32	131563.0
33	171846.0
34	262452.0
35	402809.0
36	329148.0
37	475177.0
38	552242.0
39	520619.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.954950839538547	24.11886322646002	29.680954049051582	14.245231884949847
2	31.291391120347274	26.041346149294426	29.708528405137113	12.958734325221185
3	27.602522165995257	25.21665988156045	33.614767310305	13.566050642139297
4	24.531827670925505	28.071079115916437	32.930408479173664	14.466684733984389
5	22.823904008104257	32.37992728890824	30.940528149512183	13.855640553475315
6	19.98717733269585	40.68460143812693	28.511854606219263	10.81636662295796
7	85.74707569811643	4.148609211018382	8.235649500347934	1.8686655905172618
8	53.042410069728795	40.30758424971715	4.670569286191046	1.979436394362997
9	80.66259520845267	5.597919733771367	10.23186128087026	3.507623776905708
10	43.61890346371783	29.72589551567596	14.993319431755245	11.661881588850965
11	36.50057752299441	22.886419688800313	26.70546800664625	13.907534781559027
12	33.674708989950155	21.882440934072438	27.965426727953535	16.47742334802388
13	22.012206090500694	32.985261329154966	29.218107239392747	15.784425340951588
14	17.23166482837626	35.93423811935791	29.58257987095675	17.251517181309083
15	15.551493038954401	23.842735044758033	44.126455050249234	16.479316866038335
16	17.42974456441985	21.004321954868	41.91914559733393	19.646787883378224
17	18.204933087807166	21.853446439476063	30.492592794217195	29.44902767849958
18	21.360835893527483	23.486398623412402	34.43874350878356	20.71402197427656
19	26.37670594138615	26.196082074538335	27.491041292894103	19.936170691181413
20	28.09022139959384	24.485969031512873	26.756090027314	20.667719541579288
21	22.6824818813995	28.38374627805365	28.665614186236965	20.268157654309885
22	22.318482629339115	25.315448266720946	27.31961874014779	25.04645036379215
23	19.71164087537338	29.868974470643373	26.704965040923657	23.714419613059594
24	19.861554246924218	24.980739371446695	35.75177990693359	19.405926474695498
25	18.62322305168831	26.49111585016592	33.22203983961902	21.66362125852675
26	18.02064052980634	31.771960075172668	28.937245262654613	21.270154132366375
27	19.64791215969931	29.863116399286145	29.500063906232988	20.98890753478156
28	17.485869621817116	28.266081885186534	35.29115206369794	18.956896429298403
29	18.064398547671683	25.087220173540924	34.326878014835714	22.52150326395168
30	20.1656709917774	28.52170681713823	30.102291393487246	21.210330797597127
31	25.69104531661988	26.802777080857954	25.2649445909291	22.241233011593064
32	25.94451045458634	28.336408327692226	25.834804754623736	19.8842764630977
33	23.847971805516764	27.93382864608728	25.477166539643164	22.741033008752787
34	19.883388876528425	29.228847036880996	27.386513181252276	23.5012509053383
35	20.604523377846785	27.041094074708756	27.842791850298465	24.511590697145998
36	26.77023223998447	26.877097662925394	25.423585897077828	20.92908420001231
37	21.29613083262721	31.029286806439853	27.30677832111225	20.367804039820683
38	22.420998878090575	30.665997623634894	24.816654201006404	22.096349297268127
39	21.407937154137098	30.95076498127784	25.857763660549026	21.783534204036034
40	24.3448131807789	26.496470955800554	26.14522336411878	23.013492499301766
41	19.167136101341082	26.293361562531064	27.44163230720436	27.097870028923488
42	22.634818482629342	27.436218029131776	25.299146260065232	24.629817228173653
43	22.015963540310633	27.19547496532495	26.674224959407706	24.11433653495671
44	21.338616643076588	29.643616240704013	27.506662816513373	21.511104299706034
45	19.318114576775056	31.345829763262913	25.57503775201541	23.76101790794662
46	22.77822288600548	30.079539591094782	26.202650215150985	20.93958730774875
47	21.709805346348112	27.089023749449698	27.455656174998932	23.745514729203254
48	22.876744995195196	25.014911454363848	28.30090486492116	23.807438685519795
49	21.799451589845063	24.1106678438037	29.80447651327593	24.28540405307531
50	20.563812740535962	28.666827221214973	28.02104881962821	22.748311218620856
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1762.0
1	2470.0
2	3178.0
3	18431.5
4	33685.0
5	25038.0
6	16391.0
7	16869.5
8	17348.0
9	18548.5
10	19749.0
11	20111.0
12	20473.0
13	20333.5
14	20194.0
15	19538.5
16	18883.0
17	18061.0
18	17239.0
19	16825.5
20	16412.0
21	16643.5
22	16875.0
23	17901.5
24	18928.0
25	21536.0
26	24144.0
27	29219.0
28	34294.0
29	41560.0
30	48826.0
31	55861.5
32	62897.0
33	68464.0
34	74031.0
35	83473.5
36	92916.0
37	107438.5
38	121961.0
39	153231.5
40	184502.0
41	223964.5
42	263427.0
43	287380.5
44	311334.0
45	323695.5
46	336057.0
47	331697.5
48	327338.0
49	311478.0
50	295618.0
51	262893.0
52	230168.0
53	203064.5
54	175961.0
55	158703.0
56	141445.0
57	129538.5
58	117632.0
59	107862.0
60	98092.0
61	85569.0
62	73046.0
63	61481.5
64	49917.0
65	40838.5
66	31760.0
67	26892.5
68	22025.0
69	18948.5
70	15872.0
71	13444.0
72	11016.0
73	8850.5
74	6685.0
75	5291.5
76	3898.0
77	3024.0
78	2150.0
79	1566.5
80	983.0
81	715.5
82	448.0
83	334.0
84	220.0
85	159.0
86	98.0
87	75.0
88	52.0
89	33.0
90	14.0
91	10.0
92	6.0
93	4.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3379952.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.971766327535217
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.99579264155126	21.036842936588116
2	7.588107438285017	4.093293213873108
3	2.7161696176507473	2.197796766996799
4	1.4811734331604718	1.5979945491902938
5	0.9978666294519144	1.3457112777811107
6	0.7673101350574774	1.2417425798111863
7	0.6206889277750963	1.1718753705426797
8	0.534501408696218	1.1533157677674235
9	0.4309171525425327	1.0460337070413634
>10	5.548382351454424	34.913378167772954
>50	1.068923082363405	19.76680860752747
>100	0.24711793599766496	9.072115094408218
>500	0.002228295163853353	0.38629163241579606
>1k	4.6911477133752664E-4	0.17350226038327551
>5k	2.3455738566876332E-4	0.41665848199972744
>10k+	1.1727869283438166E-4	0.3866395859005974
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12223	0.361632354542313	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7556	0.22355347058183073	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	5616	0.16615620576860263	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.9586218975890783E-5	0.0	0.0	0.05109540017136338	0.0
2	2.9586218975890783E-5	0.0	0.0	0.1705053799580586	0.0
3	2.9586218975890783E-5	0.0	0.0	0.2671339711333179	0.0
4	2.9586218975890783E-5	0.0	0.0	0.4056270621594626	0.0
5	2.9586218975890783E-5	0.0	0.0	0.6762817933509115	0.0
6	2.9586218975890783E-5	0.0	0.0	1.1251934938721024	0.0
7	2.9586218975890783E-5	0.0	0.0	1.4388074150165446	0.0
8	0.0010355176641561774	0.0	0.0	2.347577717080006	0.0
9	0.0010355176641561774	0.0	0.0	3.191642958243194	0.0
10	0.0010355176641561774	0.0	0.0	4.348907913485162	0.0
11	0.001065103883132068	0.0	0.0	5.002201214691806	0.0
12	0.001065103883132068	0.0	0.0	5.633837403608099	0.0
13	0.001065103883132068	0.0	0.0	5.944818151263687	0.0
14	0.001065103883132068	0.0	0.0	6.077660274465436	0.0
15	0.001065103883132068	0.0	0.0	6.22227771281959	0.0
16	0.001065103883132068	0.0	0.0	6.49695616979176	0.0
17	0.001065103883132068	0.0	0.0	6.801191259520845	0.0
18	0.001065103883132068	0.0	0.0	7.282056076535998	0.0
19	0.001065103883132068	0.0	0.0	7.48205891681302	0.0
20	0.001065103883132068	0.0	0.0	7.727299085904178	0.0
21	0.001065103883132068	0.0	0.0	7.980202085710093	0.0
22	0.001065103883132068	0.0	0.0	8.241803433894919	0.0
23	0.001065103883132068	0.0	0.0	8.537517692558948	0.0
24	0.001065103883132068	0.0	0.0	8.766248751461559	0.0
25	0.001065103883132068	0.0	0.0	8.956340208381658	0.0
26	0.001065103883132068	0.0	0.0	9.14258545683489	0.0
27	0.001065103883132068	0.0	0.0	9.33971843387125	0.0
28	0.001065103883132068	0.0	0.0	9.539454998177488	0.0
29	0.001065103883132068	0.0	0.0	9.750818946541253	0.0
30	0.001065103883132068	0.0	0.0	10.027509266403783	0.0
31	0.001065103883132068	0.0	0.0	10.264672397714524	0.0
32	0.001065103883132068	1.1834487590356313E-4	0.0	10.48689448844244	0.0
33	0.001065103883132068	1.1834487590356313E-4	0.0	10.708968648075476	0.0
34	0.001065103883132068	1.1834487590356313E-4	0.0	10.9322854289055	0.0
35	0.001065103883132068	1.1834487590356313E-4	0.0	11.188383740360809	0.0
36	0.001065103883132068	1.1834487590356313E-4	0.0	11.421671076985708	0.0
37	0.001065103883132068	1.1834487590356313E-4	0.0	11.666053245726566	0.0
38	0.001065103883132068	1.1834487590356313E-4	0.0	11.914015346963508	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCGAA	30	2.529181E-6	44.000004	33
CGTTTTT	16800	0.0	42.80833	1
TAATGCG	120	0.0	42.16667	1
AGCGATA	460	0.0	42.086952	8
CGACGGT	465	0.0	41.63441	28
CGGTCTA	455	0.0	41.582413	31
TACGACG	85	0.0	41.411766	1
GAGCGAT	2160	0.0	41.351852	7
CGTAGGT	70	0.0	40.857143	12
CACGACG	470	0.0	40.723408	26
TCACGAC	480	0.0	40.333336	25
GTTTTTA	7815	0.0	37.97569	2
CGTAAGA	290	0.0	37.931038	2
ACGGTCT	505	0.0	37.90099	30
TACGCGC	105	0.0	37.714287	34
GACGGTC	520	0.0	36.384613	29
ACGTGCG	115	0.0	36.347824	1
ATGTCTC	2365	0.0	36.279068	8
TCGACGA	85	0.0	36.235294	2
GCGCGAC	1055	0.0	35.8673	9
>>END_MODULE