FastQCFastQC Report
Thu 26 May 2016
SRR1546746_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546746_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2070988
Sequences flagged as poor quality0
Sequence length50
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152760.7376189528862552No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94670.4571248119255158No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT207.857946E-444.026
CGTTCGA254.444081E-544.014
CGTTTTT221100.043.4328351
GAGCGAT12350.041.5060737
CGACGGT1650.041.33333228
CTTGCGA800.041.252
GTTTTTA98250.040.506872
GGTACGA501.3496901E-939.62
CTAGCGA850.038.823532
ACGGTCT1800.037.8888930
TACGACG357.2930343E-637.7142871
TATTACG357.2930343E-637.7142871
TAGGGCG4900.037.7142875
TAGTGCG1750.037.7142871
GAGACCG4550.037.230777
TAGCGCG301.3014558E-436.6666641
GCGCCGA301.3014558E-436.66666440
AAACGCG301.3014558E-436.6666641
TCGCGCG301.3014558E-436.6666641
AGCGATA2950.036.5423748
Produced by FastQC (version 0.11.5)