FastQCFastQC Report
Thu 26 May 2016
SRR1546745_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546745_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2461305
Sequences flagged as poor quality0
Sequence length50
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186700.7585406928438369No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113510.46117811486183147No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA28490.11575160331612701No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT282550.043.2992361
CGACGGT2400.042.1666728
GAGCGAT14550.042.0343637
CGGTCTA2450.040.4081631
GTTTTTA123000.040.0113832
ACGTGCG501.3496901E-939.6000021
CACGACG2650.039.01886726
TCACGAC2750.038.425
CGCGGGT1950.038.3589744
CGCGAGT1000.037.44
TCTAGCG1000.037.41
AGCGATA4250.036.752948
ACGTTAC301.301527E-436.66666833
GAGACCG5300.036.52837
TCTCACG2900.036.41379523
GTTTTTT204600.036.3440862
GACCGAT11450.036.122279
ACGGTCT2750.036.030
ACGAGAC2150.035.8139535
GCGAGTG2650.035.6981125
Produced by FastQC (version 0.11.5)