##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546745_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2461305
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.46053170980435	31.0	31.0	33.0	30.0	34.0
2	31.85989424309462	31.0	31.0	34.0	30.0	34.0
3	31.934598515827986	31.0	31.0	34.0	30.0	34.0
4	35.670908318960876	37.0	35.0	37.0	33.0	37.0
5	35.57390571261993	37.0	35.0	37.0	33.0	37.0
6	35.6207203089418	37.0	35.0	37.0	33.0	37.0
7	36.02465440081583	37.0	35.0	37.0	35.0	37.0
8	22.215920416201975	35.0	0.0	37.0	0.0	37.0
9	30.024596301555476	37.0	18.0	39.0	17.0	39.0
10	34.29569395097316	35.0	32.0	39.0	27.0	39.0
11	35.95361566323556	37.0	35.0	39.0	32.0	39.0
12	36.50149250092939	38.0	35.0	39.0	32.0	39.0
13	36.67841124931693	39.0	35.0	39.0	33.0	39.0
14	37.85654480042091	40.0	36.0	41.0	33.0	41.0
15	38.0853587832471	40.0	37.0	41.0	33.0	41.0
16	38.11461521428673	40.0	37.0	41.0	33.0	41.0
17	38.066917752980636	40.0	36.0	41.0	33.0	41.0
18	37.94863415952107	39.0	36.0	41.0	33.0	41.0
19	37.88615429619653	40.0	36.0	41.0	33.0	41.0
20	37.711777288877244	39.0	35.0	41.0	33.0	41.0
21	37.61668748895403	39.0	35.0	41.0	33.0	41.0
22	37.58359975703946	39.0	35.0	41.0	33.0	41.0
23	37.40286474045273	39.0	35.0	41.0	33.0	41.0
24	37.19197742660906	39.0	35.0	41.0	33.0	41.0
25	37.05519064073733	39.0	35.0	41.0	32.0	41.0
26	36.99423679714623	39.0	35.0	41.0	32.0	41.0
27	36.873117309719845	39.0	35.0	41.0	32.0	41.0
28	36.85744188550383	39.0	35.0	41.0	32.0	41.0
29	36.74573813485123	38.0	35.0	40.0	31.0	41.0
30	36.594895390859726	38.0	35.0	40.0	31.0	41.0
31	36.388686489484236	38.0	35.0	40.0	31.0	41.0
32	35.890835958972986	38.0	35.0	40.0	29.0	41.0
33	35.47363370244646	38.0	35.0	41.0	25.0	41.0
34	35.117095199497825	38.0	35.0	41.0	23.0	41.0
35	34.80879045872007	38.0	34.0	41.0	21.0	41.0
36	34.624627585772586	38.0	34.0	40.0	18.0	41.0
37	34.55276245731431	38.0	34.0	40.0	18.0	41.0
38	34.44373655438883	38.0	34.0	40.0	18.0	41.0
39	34.3407107205324	38.0	34.0	40.0	18.0	41.0
40	34.26207316850208	38.0	34.0	40.0	18.0	41.0
41	34.2441720956972	38.0	34.0	40.0	17.0	41.0
42	33.997379845244694	38.0	33.0	40.0	15.0	41.0
43	34.04856001186363	38.0	33.0	40.0	15.0	41.0
44	33.8457419945923	38.0	33.0	40.0	15.0	41.0
45	33.687805452798415	38.0	33.0	40.0	15.0	41.0
46	33.7666437113645	38.0	33.0	40.0	15.0	41.0
47	33.66327375111983	38.0	33.0	40.0	15.0	41.0
48	33.650328992140345	38.0	33.0	40.0	15.0	41.0
49	33.57064565342369	38.0	33.0	40.0	14.0	41.0
50	33.12023052811415	37.0	32.0	40.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	4.0
11	0.0
12	3.0
13	6.0
14	15.0
15	50.0
16	137.0
17	388.0
18	1025.0
19	2471.0
20	4727.0
21	7935.0
22	12843.0
23	19703.0
24	30166.0
25	45992.0
26	61338.0
27	64794.0
28	58615.0
29	55313.0
30	59919.0
31	71394.0
32	88837.0
33	113364.0
34	165301.0
35	243534.0
36	224122.0
37	329824.0
38	389446.0
39	410038.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.0642971919368	23.85120901310484	26.440688984095832	20.64380481086253
2	35.185358986391364	24.860429731382336	27.79541747162582	12.158793810600473
3	24.775068510404033	23.853362342334655	39.05501349893654	12.316555648324771
4	22.050091313348	26.236082078409623	38.542521142239586	13.171305466002792
5	20.682117819611953	29.846565135162038	37.060705601296874	12.410611443929136
6	18.57368347279187	37.23983821590579	34.1680124974353	10.018465813867033
7	80.5760765122567	3.273101058178487	14.419301955669859	1.7315204738949461
8	50.54269990919451	39.44968218079433	8.398755944509112	1.6088619655020404
9	75.72316311875204	5.305112531766685	16.003380320602282	2.968344028878989
10	35.60680208263503	28.215072898320198	23.869979543372317	12.308145475672458
11	29.04170754945039	24.975409386484	31.784723957412837	14.198159106652772
12	25.79460895744331	22.435618503192412	35.3140711939398	16.45570134542448
13	19.392639270630823	30.042721239342544	35.85959480844511	14.70504468158152
14	16.66274598231426	32.490203367725655	35.388015707114725	15.45903494284536
15	15.258287778231466	25.089982753051736	44.62819520538901	15.023534263327786
16	16.82574894212623	23.190746372351253	42.97049735810881	17.01300732741371
17	17.989928107243923	23.155561785313076	35.272995423159664	23.581514684283338
18	19.829561960017145	24.298410802399538	37.987002829799636	17.885024407783675
19	22.80014057583274	26.358090525148242	33.38058468982917	17.461184209189838
20	23.878470973731414	24.459585463808832	33.52806742764509	18.13387613481466
21	20.38349574717477	27.2019111812636	34.91302378209934	17.5015692894623
22	20.15621794129537	24.95923910283366	33.622732656050346	21.261810299820624
23	17.46882243362769	28.6747477456065	33.37457161952704	20.481858201238772
24	17.784711768756818	25.15807671133809	38.95587909665807	18.10133242324702
25	16.805190742309467	27.204389541320563	37.393902827971345	18.596516888398636
26	16.70695017480564	29.991366368654027	34.93898561941734	18.36269783712299
27	17.685089820237636	29.342604837677573	34.97043235194338	18.00187299014141
28	16.63012101303983	27.282803228368692	39.05663865307225	17.030437105519226
29	16.922283097787556	25.654601928651672	37.66124068329606	19.761874290264718
30	18.136598267991978	28.603078448221574	35.00752649509102	18.252796788695427
31	21.548121829679783	27.606615189909416	32.155218471501904	18.690044508908894
32	22.279603706164007	27.945419198352095	32.58442167874359	17.190555416740306
33	21.975537367372187	29.118821113189952	30.555416740306462	18.350224779131395
34	19.02486688971907	28.706072591572358	32.365635303223286	19.90342521548528
35	19.670012452743567	28.74727024891267	31.630009283693	19.952708014650764
36	24.711078066310353	27.57533097279695	29.8049611892878	17.9086297716049
37	21.443665047606856	30.554441647825037	30.460710883047813	17.54118242152029
38	21.928326639729736	30.094319883151417	28.951958412305668	19.02539506481318
39	22.14524408799397	29.816743556771712	29.093265564405872	18.944746790828443
40	23.519027507765188	27.4263449674055	28.713223269769493	20.341404255059818
41	19.976272749618595	26.66581346074542	29.82263474051367	23.535279049122316
42	21.729204629251555	28.112119383822808	28.438937880514604	21.71973810641103
43	21.01844346799767	28.429511986527473	29.663329006360446	20.888715539114415
44	20.54430474890353	30.047068526655572	29.83807370480294	19.570553019637956
45	19.11254395534076	31.93289738573643	28.293689729635297	20.660868929287513
46	21.685934900388208	30.198532892103984	28.91027320872464	19.205258998783165
47	21.044649078436034	28.240506560544105	29.632694850902265	21.082149510117603
48	21.674518192584827	26.688768762912357	30.41991951424143	21.216793530261384
49	21.27489279061311	25.635343852143478	31.11329152624319	21.97647183100022
50	19.936700246413995	28.825277647426873	30.34195274458062	20.896069361578512
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1582.0
1	2068.5
2	2555.0
3	27587.5
4	52620.0
5	39638.5
6	26657.0
7	27412.5
8	28168.0
9	29411.0
10	30654.0
11	30950.5
12	31247.0
13	30818.0
14	30389.0
15	29170.0
16	27951.0
17	26628.5
18	25306.0
19	24332.0
20	23358.0
21	22805.0
22	22252.0
23	22873.0
24	23494.0
25	25769.5
26	28045.0
27	31854.5
28	35664.0
29	40655.5
30	45647.0
31	51354.0
32	57061.0
33	64738.5
34	72416.0
35	80566.5
36	88717.0
37	99748.0
38	110779.0
39	127776.0
40	144773.0
41	164207.0
42	183641.0
43	196690.0
44	209739.0
45	218107.0
46	226475.0
47	224668.0
48	222861.0
49	209458.5
50	196056.0
51	171493.0
52	146930.0
53	127555.0
54	108180.0
55	93952.5
56	79725.0
57	70167.5
58	60610.0
59	53209.5
60	45809.0
61	38505.5
62	31202.0
63	24868.0
64	18534.0
65	14211.5
66	9889.0
67	7495.0
68	5101.0
69	4064.0
70	3027.0
71	2447.0
72	1867.0
73	1463.5
74	1060.0
75	805.0
76	550.0
77	450.5
78	351.0
79	291.0
80	231.0
81	147.5
82	64.0
83	43.5
84	23.0
85	21.5
86	20.0
87	18.0
88	16.0
89	9.0
90	2.0
91	2.5
92	3.0
93	3.0
94	3.0
95	1.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2461305.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.27322808722751
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.03328709970492	27.5797276518925
2	7.24250360787991	5.2541797058239235
3	3.297553107896783	3.5883868803745793
4	2.084000224040597	3.0237366184183125
5	1.5418045057202088	2.79631132509521
6	1.2267234227741668	2.6698331108539066
7	1.0017828602686907	2.5436528729080967
8	0.8027908756909994	2.329585323222778
9	0.7179658811358088	2.3438646148757827
>10	5.84931906724147	40.22164466054577
>50	0.1772507802274172	4.046035267892938
>100	0.022174562753107207	1.361348441763273
>500	0.0013035021385444647	0.28558467151701694
>1k	0.0013035021385444418	0.6656908199554449
>5k	0.0	0.0
>10k+	2.3700038882626216E-4	1.2904180348603609
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18670	0.7585406928438369	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11351	0.46117811486183147	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2849	0.11575160331612701	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.087798952181871	0.0
2	0.0	0.0	0.0	0.24438255315777604	0.0
3	0.0	0.0	0.0	0.3860147360851256	0.0
4	0.0	0.0	0.0	0.5159864380887375	0.0
5	0.0	0.0	0.0	0.7915719506521947	0.0
6	0.0	0.0	0.0	1.269367266551687	0.0
7	0.0	0.0	0.0	1.598948525274194	0.0
8	0.0	0.0	0.0	2.323767269801995	0.0
9	0.0	0.0	0.0	3.0401758416774842	0.0
10	0.0	0.0	0.0	4.034404513053035	0.0
11	0.0	0.0	0.0	4.604549212714393	0.0
12	0.0	0.0	0.0	5.168193295832902	0.0
13	0.0	0.0	0.0	5.4846108060561365	0.0
14	0.0	0.0	0.0	5.629980843495625	0.0
15	0.0	0.0	0.0	5.7749445924011855	0.0
16	0.0	0.0	0.0	6.018270795370748	0.0
17	0.0	0.0	0.0	6.292881215452778	0.0
18	0.0	0.0	0.0	6.778558528910477	0.0
19	0.0	0.0	0.0	6.965816914197956	0.0
20	0.0	0.0	0.0	7.1950855338936055	0.0
21	0.0	0.0	0.0	7.4107434877026614	0.0
22	0.0	0.0	0.0	7.638061922435456	0.0
23	0.0	0.0	0.0	7.899386707458035	0.0
24	0.0	0.0	0.0	8.098874377616752	0.0
25	0.0	0.0	0.0	8.277194415157812	0.0
26	0.0	0.0	0.0	8.445844785591383	0.0
27	0.0	0.0	0.0	8.641635230091355	0.0
28	0.0	0.0	0.0	8.820727215846878	0.0
29	0.0	0.0	0.0	9.007985601134358	0.0
30	0.0	0.0	0.0	9.268091520555153	0.0
31	0.0	0.0	0.0	9.477248857821358	0.0
32	0.0	0.0	0.0	9.672958044614544	0.0
33	0.0	0.0	0.0	9.869601695035763	0.0
34	0.0	0.0	0.0	10.072502188879476	0.0
35	0.0	0.0	0.0	10.29433572840424	0.0
36	0.0	0.0	0.0	10.496870562567418	0.0
37	0.0	0.0	0.0	10.7157788246479	0.0
38	0.0	0.0	0.0	10.926764460316782	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	28255	0.0	43.299236	1
CGACGGT	240	0.0	42.16667	28
GAGCGAT	1455	0.0	42.034363	7
CGGTCTA	245	0.0	40.40816	31
GTTTTTA	12300	0.0	40.011383	2
ACGTGCG	50	1.3496901E-9	39.600002	1
CACGACG	265	0.0	39.018867	26
TCACGAC	275	0.0	38.4	25
CGCGGGT	195	0.0	38.358974	4
CGCGAGT	100	0.0	37.4	4
TCTAGCG	100	0.0	37.4	1
AGCGATA	425	0.0	36.75294	8
ACGTTAC	30	1.301527E-4	36.666668	33
GAGACCG	530	0.0	36.5283	7
TCTCACG	290	0.0	36.413795	23
GTTTTTT	20460	0.0	36.344086	2
GACCGAT	1145	0.0	36.12227	9
ACGGTCT	275	0.0	36.0	30
ACGAGAC	215	0.0	35.813953	5
GCGAGTG	265	0.0	35.698112	5
>>END_MODULE