FastQCFastQC Report
Thu 26 May 2016
SRR1546744_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546744_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2470168
Sequences flagged as poor quality0
Sequence length50
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151040.6114563867720738No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94390.38211975865609143No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA32010.1295863277315551No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGGA207.858241E-444.02
CGTTTTT219850.043.11941
GCGATAC950.041.684219
CGGTCTA3550.041.52112631
GCCGACC700.040.85714314
GAGCGAT17650.040.2606247
GTTTTTA99050.039.6688582
GCGCGAC4000.039.6000029
GCGAGGT2750.039.25
CGACGGT3800.038.78947428
CGGCTAC4550.038.68131625
ATCATCG404.127105E-738.527
CGAGGTA1500.038.133336
GCGGCTA4650.037.84946424
CTCACGA3900.037.7948724
TAGGTCG357.2936054E-637.7142871
TATCGCG700.037.7142871
CGAGGTT2000.037.46
GCGAAGA2900.037.1724132
TCACGAC3850.037.1428625
Produced by FastQC (version 0.11.5)