##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546744_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2470168
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.477547276136683	31.0	31.0	33.0	30.0	34.0
2	31.874364820530424	31.0	31.0	34.0	30.0	34.0
3	31.971983686939513	31.0	31.0	34.0	30.0	34.0
4	35.68572340018979	37.0	35.0	37.0	33.0	37.0
5	35.57741902575047	37.0	35.0	37.0	33.0	37.0
6	35.633025365076385	37.0	35.0	37.0	33.0	37.0
7	36.03733065929119	37.0	35.0	37.0	35.0	37.0
8	21.90709295885948	35.0	0.0	37.0	0.0	37.0
9	29.86353478791726	37.0	18.0	39.0	17.0	39.0
10	34.284810587781884	35.0	32.0	39.0	27.0	39.0
11	35.91195416668016	37.0	35.0	39.0	32.0	39.0
12	36.429688992813446	37.0	35.0	39.0	32.0	39.0
13	36.58297330384006	39.0	35.0	39.0	33.0	39.0
14	37.73004629644623	40.0	36.0	41.0	33.0	41.0
15	37.981753872611094	40.0	36.0	41.0	33.0	41.0
16	38.02853368677758	40.0	36.0	41.0	33.0	41.0
17	37.98208097586885	40.0	36.0	41.0	33.0	41.0
18	37.898669645141545	39.0	36.0	41.0	33.0	41.0
19	37.86749484245606	40.0	36.0	41.0	33.0	41.0
20	37.707944156025015	39.0	35.0	41.0	33.0	41.0
21	37.618699618811355	39.0	35.0	41.0	33.0	41.0
22	37.57866954798216	39.0	35.0	41.0	33.0	41.0
23	37.41397184321067	39.0	35.0	41.0	33.0	41.0
24	37.251401928937625	39.0	35.0	41.0	32.0	41.0
25	37.115444374633626	39.0	35.0	41.0	32.0	41.0
26	37.034031693390894	39.0	35.0	41.0	32.0	41.0
27	36.91310712469759	39.0	35.0	41.0	32.0	41.0
28	36.90349401336265	39.0	35.0	41.0	32.0	41.0
29	36.80666861525208	39.0	35.0	41.0	31.0	41.0
30	36.677920287203136	38.0	35.0	40.0	31.0	41.0
31	36.541831162900664	38.0	35.0	40.0	31.0	41.0
32	36.153151526535844	39.0	35.0	41.0	30.0	41.0
33	35.8690668003148	39.0	35.0	41.0	28.0	41.0
34	35.65380168474371	39.0	35.0	41.0	26.0	41.0
35	35.415614241622436	39.0	35.0	41.0	25.0	41.0
36	35.2566469163231	39.0	35.0	41.0	24.0	41.0
37	35.21748075434545	39.0	35.0	41.0	24.0	41.0
38	35.12420774619378	38.0	35.0	40.0	23.0	41.0
39	35.036618561976354	38.0	35.0	40.0	23.0	41.0
40	34.98220647340586	38.0	35.0	40.0	23.0	41.0
41	34.974196491898525	38.0	35.0	40.0	23.0	41.0
42	34.71955227336764	38.0	34.0	40.0	21.0	41.0
43	34.76953267955864	38.0	34.0	40.0	22.0	41.0
44	34.551619161125885	38.0	34.0	40.0	21.0	41.0
45	34.36771142691509	38.0	34.0	40.0	20.0	41.0
46	34.463065670027305	38.0	34.0	40.0	20.0	41.0
47	34.34829412412435	38.0	34.0	40.0	20.0	41.0
48	34.35537906733469	38.0	34.0	40.0	20.0	41.0
49	34.28149057068183	38.0	34.0	40.0	20.0	41.0
50	33.818756457050696	37.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	2.0
12	2.0
13	6.0
14	17.0
15	34.0
16	135.0
17	373.0
18	1009.0
19	2197.0
20	4250.0
21	6925.0
22	10980.0
23	16283.0
24	24697.0
25	37130.0
26	49592.0
27	53528.0
28	51983.0
29	52183.0
30	58630.0
31	70826.0
32	89843.0
33	117027.0
34	172760.0
35	264450.0
36	226989.0
37	337814.0
38	405599.0
39	414903.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.711298988570817	24.26899708845714	28.997906215285763	17.02179770768628
2	33.997930505131635	24.941218572987747	28.039874210984838	13.020976710895777
3	26.132959377661763	24.522259214757863	36.27222925728128	13.072552150299089
4	23.605479465364297	26.48083045363716	36.04997716754488	13.86371291345366
5	21.190218640999316	30.91728983615689	34.54963387105654	13.342857651787247
6	19.320143407250036	39.180897817476385	31.49247338642554	10.006485388848047
7	83.66406657360956	3.3239439584676025	11.38019762218602	1.6317918457368081
8	51.7338091984027	40.191436371939076	6.507695023172514	1.5670594064857126
9	79.03397663640692	5.352186571925472	12.916125542878055	2.697711248789556
10	39.04386260367716	31.474579866632553	18.836734991304237	10.644822538386054
11	30.835837886329998	23.641145055720905	31.908477480074232	13.614539577874865
12	28.571214589453025	22.994711290891956	32.43815805240777	15.995916067247249
13	20.11761953033154	31.71500885769713	33.66568589666776	14.501685715303575
14	16.38487746582419	34.352197907186884	33.27093541815779	15.99198920883114
15	14.977766694411068	25.186100702462344	44.43531775976371	15.40081484336288
16	16.889418047679346	23.31319165336123	42.54402939395215	17.25336090500727
17	17.530346114110458	23.255341337107435	34.05084188605795	25.16347066272415
18	19.464303642505286	25.14756081367745	36.62605134549553	18.762084198321734
19	23.61636941293062	26.568962111079088	31.562023311774745	18.25264516421555
20	24.737102901503054	25.76970473263357	30.778756748528846	18.71443561733453
21	21.235519203552148	27.931420049162647	32.80833530350972	18.024725443775484
22	20.634912281269937	25.352607595920603	31.86985662513643	22.142623497673032
23	17.699281992156	29.48868255114632	31.823260604137047	20.988774852560635
24	18.00059753020847	25.72861441003203	38.33941659028859	17.931371469470903
25	17.001961000223467	27.517521075489604	36.270043171152736	19.2104747531342
26	16.741938200154806	31.349770541922656	33.054350959125046	18.853940298797493
27	17.720333191912452	30.46987087517934	32.92614105599295	18.883654876915255
28	16.534300501018553	28.396894462239004	37.78107399982512	17.28773103691733
29	16.93876691787765	25.58651881167597	37.09699097389327	20.37772329655311
30	18.265437816375243	28.375033600953458	33.75312934180995	19.60639924086135
31	22.395480793209206	27.48618717431365	30.498978207150284	19.61935382532686
32	23.363350185088624	27.819767724300533	30.313808615446398	18.50307347516444
33	22.037731846578858	28.86115438302172	29.92480673379301	19.176307036606417
34	18.50331637362317	29.09166502035489	30.933725965197507	21.47129264082443
35	18.697513691376457	27.91615792933922	32.138826185101586	21.247502194182744
36	23.895581191238815	27.344779788257316	29.663771856812975	19.09586716369089
37	19.680807135385123	30.287454132674373	31.05108640383974	18.98065232810076
38	19.78646796493194	30.73989299513232	29.39431649993037	20.079322540005375
39	20.4398648189111	29.630130420279105	29.85416376537952	20.07584099543027
40	21.774875231158365	26.75477943200624	29.9723743486273	21.49797098820809
41	18.89839071674477	26.537992557591224	29.6034925559719	24.9601241696921
42	20.789395701021146	27.837539794864153	28.44162826172147	22.93143624239323
43	20.975213021948306	28.17095841254522	29.07988444510657	21.773944120399907
44	19.987021125688617	30.50545549938304	29.5738994270835	19.933623947844843
45	18.149899116173472	32.23825262087437	28.24888833472055	21.3629599282316
46	20.98359301877443	30.536141671335713	28.54619604820401	19.934069261685845
47	20.243886245793806	28.016313060488194	29.468724394454142	22.271076299263857
48	20.778060439613824	26.277200579069927	30.85369092304653	22.09104805826972
49	20.827287860582764	25.135780238429128	31.396609461380763	22.640322439607345
50	18.92239718108242	29.374763174002737	30.11366028545427	21.589179359460573
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1502.0
1	2018.0
2	2534.0
3	22314.0
4	42094.0
5	31324.5
6	20555.0
7	21211.5
8	21868.0
9	23149.0
10	24430.0
11	24789.0
12	25148.0
13	24574.0
14	24000.0
15	23012.5
16	22025.0
17	20927.0
18	19829.0
19	19307.5
20	18786.0
21	19034.0
22	19282.0
23	20822.5
24	22363.0
25	25409.0
26	28455.0
27	32738.0
28	37021.0
29	42772.5
30	48524.0
31	56492.5
32	64461.0
33	72987.0
34	81513.0
35	89043.0
36	96573.0
37	105163.5
38	113754.0
39	132123.5
40	150493.0
41	173333.5
42	196174.0
43	210199.0
44	224224.0
45	228548.5
46	232873.0
47	228028.0
48	223183.0
49	207027.5
50	190872.0
51	167330.0
52	143788.0
53	125682.5
54	107577.0
55	95073.0
56	82569.0
57	72426.5
58	62284.0
59	53901.0
60	45518.0
61	38805.0
62	32092.0
63	25366.0
64	18640.0
65	14350.5
66	10061.0
67	8046.0
68	6031.0
69	4916.0
70	3801.0
71	3148.0
72	2495.0
73	1934.5
74	1374.0
75	1026.5
76	679.0
77	517.5
78	356.0
79	269.0
80	182.0
81	134.5
82	87.0
83	76.5
84	66.0
85	39.5
86	13.0
87	9.5
88	6.0
89	7.5
90	9.0
91	5.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2470168.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.5625195993405
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.80367276617727	25.777247725161583
2	7.175906906969887	4.816830324164393
3	3.0774002901551967	3.0985592266004973
4	1.8544113144591172	2.489548643470916
5	1.323960789164259	2.2217729967541877
6	1.0487291031115389	2.1118794646547885
7	0.8616890511243255	2.02443189668281
8	0.7031255333555692	1.8878931595234423
9	0.6160336219993121	1.8608076460984162
>10	6.082268351107516	41.30019524805862
>50	0.4175371441320254	8.946404479422183
>100	0.03305371354353126	1.7811048648945276
>500	0.0015609986121427688	0.3630699523460739
>1k	3.9024965303569205E-4	0.24872649332799215
>5k	1.3008321767856402E-4	0.41209924004263965
>10k+	1.3008321767856402E-4	0.6594286387969095
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15104	0.6114563867720738	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9439	0.38211975865609143	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3201	0.1295863277315551	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.059672054694255613	0.0
2	0.0	0.0	0.0	0.1761418656544818	0.0
3	0.0	0.0	0.0	0.2737870460632637	0.0
4	0.0	0.0	0.0	0.393981300057324	0.0
5	0.0	0.0	0.0	0.6484174355752321	0.0
6	0.0	0.0	0.0	1.072680076820686	0.0
7	0.0	0.0	0.0	1.3798656609590927	0.0
8	0.0	0.0	0.0	2.1043103141162867	0.0
9	0.0	0.0	0.0	2.8281072380502055	0.0
10	0.0	0.0	0.0	3.777556830142727	0.0
11	0.0	0.0	0.0	4.3389356513403134	0.0
12	0.0	0.0	0.0	4.849062897746226	0.0
13	0.0	0.0	0.0	5.130703660641705	0.0
14	0.0	0.0	0.0	5.259804191455804	0.0
15	0.0	0.0	0.0	5.390807426863274	0.0
16	0.0	0.0	0.0	5.634920377885229	0.0
17	0.0	0.0	0.0	5.9126342823646	0.0
18	0.0	0.0	0.0	6.344912572748088	0.0
19	0.0	0.0	0.0	6.5342924044032635	0.0
20	0.0	0.0	0.0	6.75431792493466	0.0
21	0.0	0.0	0.0	6.975557937759699	0.0
22	0.0	0.0	0.0	7.2123029688668945	0.0
23	0.0	0.0	0.0	7.48661629492407	0.0
24	0.0	0.0	0.0	7.687048006451383	0.0
25	0.0	0.0	0.0	7.862258761347406	0.0
26	0.0	0.0	0.0	8.032773479374683	0.0
27	0.0	0.0	0.0	8.220088674130666	0.0
28	0.0	0.0	0.0	8.407646767345378	0.0
29	0.0	0.0	0.0	8.606135291202866	0.0
30	0.0	0.0	0.0	8.860976257485321	0.0
31	0.0	0.0	0.0	9.088167282549204	0.0
32	0.0	0.0	0.0	9.296250295526459	0.0
33	0.0	0.0	0.0	9.500892247005062	0.0
34	0.0	0.0	0.0	9.707760767688676	0.0
35	0.0	0.0	0.0	9.94183391574986	0.0
36	0.0	0.0	0.0	10.165381463932817	0.0
37	0.0	0.0	0.0	10.40107393505219	0.0
38	4.048307645471887E-5	0.0	0.0	10.634944667731101	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCGGA	20	7.858241E-4	44.0	2
CGTTTTT	21985	0.0	43.1194	1
GCGATAC	95	0.0	41.68421	9
CGGTCTA	355	0.0	41.521126	31
GCCGACC	70	0.0	40.857143	14
GAGCGAT	1765	0.0	40.260624	7
GTTTTTA	9905	0.0	39.668858	2
GCGCGAC	400	0.0	39.600002	9
GCGAGGT	275	0.0	39.2	5
CGACGGT	380	0.0	38.789474	28
CGGCTAC	455	0.0	38.681316	25
ATCATCG	40	4.127105E-7	38.5	27
CGAGGTA	150	0.0	38.13333	6
GCGGCTA	465	0.0	37.849464	24
CTCACGA	390	0.0	37.79487	24
TAGGTCG	35	7.2936054E-6	37.714287	1
TATCGCG	70	0.0	37.714287	1
CGAGGTT	200	0.0	37.4	6
GCGAAGA	290	0.0	37.172413	2
TCACGAC	385	0.0	37.14286	25
>>END_MODULE