FastQCFastQC Report
Thu 26 May 2016
SRR1546743_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546743_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2750595
Sequences flagged as poor quality0
Sequence length50
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68340.2484553342095074No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42740.15538456224925878No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA29460.10710409929487984No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCGC207.8583986E-444.012
CGCAATA207.8583986E-444.031
GCTCGTC254.4444634E-544.011
TCGCGAC207.8583986E-444.011
CGTTTTT90850.042.4986231
CGGTCTA3100.041.1612931
TATAGCG1450.040.965521
CACGACG3400.040.76470626
GAGCGAT21200.039.9528277
CGACGGT3200.039.87528
AGCGATC2550.038.8235328
AGCGATA5400.038.7037058
CCGTAAG750.038.133331
AACGCGA1350.037.481482
CGTTAGA2350.037.4468082
ACGAGAT3900.037.2307665
CGTACTG301.3015668E-436.66666429
TATCGCG850.036.2352941
AGGGGAA185900.036.2011838
TTTAGCG1400.036.1428571
Produced by FastQC (version 0.11.5)