##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546743_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2750595
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.576006282277106	31.0	31.0	33.0	30.0	34.0
2	31.969931596618185	31.0	31.0	34.0	30.0	34.0
3	32.10505545163865	33.0	31.0	34.0	30.0	34.0
4	35.7890067421776	37.0	35.0	37.0	35.0	37.0
5	35.68894257424303	37.0	35.0	37.0	33.0	37.0
6	35.7378218167342	37.0	35.0	37.0	33.0	37.0
7	36.07521427182119	37.0	35.0	37.0	35.0	37.0
8	22.052381393843877	35.0	0.0	37.0	0.0	37.0
9	30.005930716808546	37.0	18.0	39.0	17.0	39.0
10	34.44400393369435	37.0	32.0	39.0	27.0	39.0
11	36.01812153370452	37.0	35.0	39.0	32.0	39.0
12	36.36450876992069	37.0	35.0	39.0	32.0	39.0
13	36.47081813207688	39.0	35.0	39.0	33.0	39.0
14	37.58584415371947	39.0	36.0	41.0	33.0	41.0
15	37.867900217952844	40.0	36.0	41.0	33.0	41.0
16	37.93869508233673	40.0	36.0	41.0	33.0	41.0
17	37.90414219468879	40.0	36.0	41.0	33.0	41.0
18	37.875007043930495	40.0	36.0	41.0	33.0	41.0
19	37.878476838647636	40.0	36.0	41.0	33.0	41.0
20	37.77762629540154	40.0	35.0	41.0	33.0	41.0
21	37.69102430565023	39.0	35.0	41.0	33.0	41.0
22	37.63660844290054	39.0	35.0	41.0	33.0	41.0
23	37.53466104606458	39.0	35.0	41.0	33.0	41.0
24	37.45009279810368	39.0	35.0	41.0	33.0	41.0
25	37.307133911026526	39.0	35.0	41.0	33.0	41.0
26	37.2355635780622	39.0	35.0	41.0	33.0	41.0
27	37.13947600428271	39.0	35.0	41.0	32.0	41.0
28	37.18040860250237	39.0	35.0	41.0	32.0	41.0
29	37.118038097211695	39.0	35.0	41.0	32.0	41.0
30	37.03901046864406	39.0	35.0	41.0	32.0	41.0
31	36.95973925641543	39.0	35.0	41.0	32.0	41.0
32	36.74378670796682	39.0	35.0	41.0	31.0	41.0
33	36.64652593347985	39.0	35.0	41.0	31.0	41.0
34	36.57889983803504	39.0	35.0	41.0	31.0	41.0
35	36.43939256778988	39.0	35.0	41.0	30.0	41.0
36	36.305117983563555	39.0	35.0	41.0	30.0	41.0
37	36.280814514677736	39.0	35.0	41.0	30.0	41.0
38	36.18910962900754	39.0	35.0	41.0	30.0	41.0
39	36.13500533520929	39.0	35.0	41.0	30.0	41.0
40	36.0605250863904	38.0	35.0	40.0	30.0	41.0
41	36.06760500909803	38.0	35.0	40.0	30.0	41.0
42	35.84428096466401	38.0	35.0	40.0	29.0	41.0
43	35.89114609748073	38.0	35.0	40.0	30.0	41.0
44	35.7129577418704	38.0	35.0	40.0	29.0	41.0
45	35.52249313330388	38.0	35.0	40.0	28.0	41.0
46	35.59155455456001	38.0	35.0	40.0	29.0	41.0
47	35.472748623479646	38.0	35.0	40.0	28.0	41.0
48	35.47822162114015	38.0	35.0	40.0	28.0	41.0
49	35.411672383611545	38.0	34.0	40.0	28.0	41.0
50	34.96494867474129	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	4.0
13	6.0
14	13.0
15	26.0
16	90.0
17	230.0
18	592.0
19	1324.0
20	2578.0
21	4444.0
22	7070.0
23	10647.0
24	15874.0
25	23188.0
26	31229.0
27	36483.0
28	41122.0
29	47421.0
30	58425.0
31	76166.0
32	99844.0
33	133930.0
34	215001.0
35	348695.0
36	264257.0
37	400080.0
38	476418.0
39	455433.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.899136368676594	24.44892832278107	29.636315051834238	13.015620256708093
2	30.38553476611424	25.43493316900525	30.822458413543252	13.357073651337256
3	28.474348277372712	25.087044803033525	32.6818015738413	13.756805345752465
4	24.851495767279445	28.67321434089715	31.835693731719868	14.63959616010354
5	22.754458580779797	32.38764703636849	30.964100494620254	13.893793888231457
6	20.733223175349334	41.49658528427486	27.43977212203178	10.33041941834403
7	89.5562596456403	3.20177997851374	5.77980400604233	1.4621563698036242
8	55.63723485282275	39.79379007087557	3.2672930765888832	1.3016819997127895
9	86.08599230348342	4.415771860270232	7.142236497921359	2.3559993383249807
10	48.05327574579319	28.08839541990006	12.948725639361665	10.909603194945094
11	38.98094775857587	22.88057674793999	23.630487221855635	14.507988271628502
12	35.18525991649079	22.372613925350695	26.330812060663238	16.11131409749527
13	21.381737405906723	35.89808750470353	26.68931631156168	16.03085877782807
14	17.41285067412687	37.75724888615009	28.07250794828028	16.75739249144276
15	16.469418434920442	24.146702804302343	42.893882959868684	16.48999580090853
16	18.117752704414862	21.205920900750566	41.827168303585225	18.84915809124935
17	18.420269069055966	21.388790425344332	28.890476424191856	31.300464081407842
18	21.523415842754023	24.068247052001475	33.31315588081851	21.09518122442599
19	27.341102561445794	26.03094966725381	26.022333349693426	20.605614421606962
20	29.298279099612994	24.65724688658272	25.782530688814603	20.261943324989684
21	22.985245010624975	28.574653847622063	28.190809624826628	20.249291516926338
22	22.95099787500523	25.263261221662948	26.677936955458726	25.107803947873098
23	20.500873447381384	30.215462472665006	25.86447659506398	23.419187484889633
24	21.05359022320625	25.103732101599835	35.18569618573436	18.656981489459554
25	18.289351940216573	26.204330335800073	33.69929051714266	21.8070272068407
26	18.089140713191146	32.63657499559186	28.1935726633692	21.08071162784779
27	19.816221581148806	30.676562707341503	28.840050970789953	20.667164740719734
28	17.92364924679933	27.95729651220918	35.85558760922637	18.263466631765127
29	18.380095942877812	24.75609822602019	35.18984074354821	21.673965087553785
30	20.38758886713602	28.065527640383266	31.44374217214821	20.10314132033251
31	27.21749294243609	26.177463421550613	25.95031256873513	20.65473106727817
32	27.92312208813002	25.719453427349354	27.520118374388087	18.837306110132534
33	26.38567291804137	26.59602740497965	26.911813625779153	20.10648605119983
34	20.581692324751554	26.64779802188254	29.244581626884365	23.525928026481544
35	21.241367776790113	25.512661805900176	30.584146339246598	22.66182407806311
36	28.32648935957493	23.930422326805655	28.140020613721756	19.603067699897657
37	22.279434086079558	29.418943901228644	29.044879380643096	19.256742632048702
38	21.374029982603766	30.49380225005862	26.35193476320578	21.780233004131833
39	21.243512767237636	29.245526876912088	28.41716065069558	21.0937997051547
40	23.409662273071827	25.55348933594368	27.596065578538457	23.440782812446034
41	19.163417369696376	24.027455877728272	28.7026261590674	28.10650059350795
42	22.020362866943337	26.27438790516234	26.532550230041135	25.172698997853193
43	22.008001905042363	26.29492891538013	27.72480136116004	23.97226781841747
44	21.158476620513017	28.62300702211703	28.581306953586406	21.637209403783544
45	19.14294907101918	32.68023827571852	25.218652691508563	22.95815996175373
46	22.349309876590336	29.667617370059933	27.54476758664943	20.438305166700296
47	21.558171959157928	26.624312194270694	28.629914618473457	23.187601228097922
48	22.797903726284677	23.860328401673094	30.269378080015414	23.072389792026815
49	21.367158742017637	23.19516322831969	31.68747125621911	23.750206773443562
50	20.214280910130352	29.145766643217197	28.432102872287636	22.20784957436482
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1090.0
1	1800.5
2	2511.0
3	11092.0
4	19673.0
5	14452.0
6	9231.0
7	9700.0
8	10169.0
9	10967.0
10	11765.0
11	12214.0
12	12663.0
13	12484.0
14	12305.0
15	11955.0
16	11605.0
17	11282.0
18	10959.0
19	11018.0
20	11077.0
21	11665.0
22	12253.0
23	13950.5
24	15648.0
25	18068.0
26	20488.0
27	24519.0
28	28550.0
29	35117.0
30	41684.0
31	48601.5
32	55519.0
33	63422.0
34	71325.0
35	79290.0
36	87255.0
37	98058.5
38	108862.0
39	135041.0
40	161220.0
41	191413.0
42	221606.0
43	237414.0
44	253222.0
45	258309.0
46	263396.0
47	255609.5
48	247823.0
49	240328.0
50	232833.0
51	210562.0
52	188291.0
53	168273.0
54	148255.0
55	134333.5
56	120412.0
57	108792.5
58	97173.0
59	89071.5
60	80970.0
61	71418.5
62	61867.0
63	52262.0
64	42657.0
65	35673.0
66	28689.0
67	23383.0
68	18077.0
69	15179.0
70	12281.0
71	9989.5
72	7698.0
73	6004.0
74	4310.0
75	3397.0
76	2484.0
77	1993.0
78	1502.0
79	1083.0
80	664.0
81	483.5
82	303.0
83	219.0
84	135.0
85	104.5
86	74.0
87	42.5
88	11.0
89	9.5
90	8.0
91	4.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2750595.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.786338778219633
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.29220816930346	18.384986484317274
2	6.75783106225	3.2148811810530873
3	2.4020561739002857	1.7140836575011875
4	1.395706465043051	1.3279498724986147
5	0.9613675008132495	1.1433706532357146
6	0.7794668102189519	1.1124396968547732
7	0.6259462323969834	1.0422278398521392
8	0.5175981993793457	0.9849412897146871
9	0.46191641662365135	0.9888570335728273
>10	7.2654468367789935	42.640429143047676
>50	1.4022153504977835	21.990955041960692
>100	0.13577833044144094	4.534895825073935
>500	0.0016416349019056928	0.2662325634134553
>1k	6.566539607622771E-4	0.3868923787475088
>5k	1.6416349019056928E-4	0.2668573391562854
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6834	0.2484553342095074	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4274	0.15538456224925878	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2946	0.10710409929487984	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06387708841178	0.0
2	0.0	0.0	0.0	0.17410778395219945	0.0
3	0.0	0.0	0.0	0.2522726900906895	0.0
4	0.0	0.0	0.0	0.35148758723112633	0.0
5	0.0	0.0	0.0	0.5405375927753814	0.0
6	0.0	0.0	0.0	0.8103701199195084	0.0
7	0.0	0.0	0.0	0.971135336172719	0.0
8	0.0	0.0	0.0	1.4264550033719976	0.0
9	0.0	0.0	0.0	1.8069908510704047	0.0
10	3.635577029697211E-5	0.0	0.0	2.3603983865309144	0.0
11	3.635577029697211E-5	0.0	0.0	2.7849247162886575	0.0
12	3.635577029697211E-5	0.0	0.0	3.134739938086123	0.0
13	3.635577029697211E-5	0.0	0.0	3.3037579141967464	0.0
14	3.635577029697211E-5	0.0	0.0	3.375815050925345	0.0
15	3.635577029697211E-5	0.0	0.0	3.457033841768781	0.0
16	3.635577029697211E-5	0.0	0.0	3.6349953373724593	0.0
17	3.635577029697211E-5	0.0	0.0	3.8433866127147036	0.0
18	3.635577029697211E-5	0.0	0.0	4.137541150187505	0.0
19	3.635577029697211E-5	0.0	0.0	4.28816310652786	0.0
20	3.635577029697211E-5	0.0	0.0	4.443911226480089	0.0
21	3.635577029697211E-5	0.0	0.0	4.634960799390678	0.0
22	3.635577029697211E-5	0.0	0.0	4.83844404574283	0.0
23	3.635577029697211E-5	0.0	0.0	5.070103014075136	0.0
24	3.635577029697211E-5	0.0	0.0	5.249009759706536	0.0
25	3.635577029697211E-5	0.0	0.0	5.397195879436994	0.0
26	3.635577029697211E-5	0.0	0.0	5.541710066367458	0.0
27	3.635577029697211E-5	0.0	0.0	5.692368378478111	0.0
28	3.635577029697211E-5	0.0	0.0	5.848625479214498	0.0
29	3.635577029697211E-5	0.0	0.0	6.018152436109278	0.0
30	3.635577029697211E-5	0.0	0.0	6.226616422992116	0.0
31	3.635577029697211E-5	0.0	0.0	6.415448293914589	0.0
32	3.635577029697211E-5	0.0	0.0	6.5873020201083765	0.0
33	3.635577029697211E-5	0.0	0.0	6.760064640559588	0.0
34	3.635577029697211E-5	0.0	0.0	6.935844789945448	0.0
35	3.635577029697211E-5	0.0	0.0	7.140055151703541	0.0
36	3.635577029697211E-5	0.0	0.0	7.326123984083444	0.0
37	7.271154059394422E-5	0.0	0.0	7.517100845453438	0.0
38	7.271154059394422E-5	0.0	0.0	7.7056055144432385	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATCGC	20	7.8583986E-4	44.0	12
CGCAATA	20	7.8583986E-4	44.0	31
GCTCGTC	25	4.4444634E-5	44.0	11
TCGCGAC	20	7.8583986E-4	44.0	11
CGTTTTT	9085	0.0	42.498623	1
CGGTCTA	310	0.0	41.16129	31
TATAGCG	145	0.0	40.96552	1
CACGACG	340	0.0	40.764706	26
GAGCGAT	2120	0.0	39.952827	7
CGACGGT	320	0.0	39.875	28
AGCGATC	255	0.0	38.823532	8
AGCGATA	540	0.0	38.703705	8
CCGTAAG	75	0.0	38.13333	1
AACGCGA	135	0.0	37.48148	2
CGTTAGA	235	0.0	37.446808	2
ACGAGAT	390	0.0	37.230766	5
CGTACTG	30	1.3015668E-4	36.666664	29
TATCGCG	85	0.0	36.235294	1
AGGGGAA	18590	0.0	36.201183	8
TTTAGCG	140	0.0	36.142857	1
>>END_MODULE