FastQCFastQC Report
Thu 26 May 2016
SRR1546742_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546742_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2667248
Sequences flagged as poor quality0
Sequence length50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA71450.26787910235568646No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55500.20807963863877674No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34450.12915934326316864No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTGC207.858353E-444.00000427
ACGTGCG207.858353E-444.00000414
TCTATCG207.858353E-444.00000436
TAATCGC207.858353E-444.00000442
ATCGTTC302.528981E-644.017
TATTACG254.444427E-544.01
TCTAGCG1250.042.241
TATGCGA1200.042.1666642
ACGTTAG900.041.5555531
TAATGCG850.041.4117621
CGTTTTT73450.041.1245731
TAACGCC2250.041.06666612
CACGACG3200.040.56250426
TACACGA603.6379788E-1240.3333322
TACGATC557.8216544E-1140.00000411
CGAGTTA501.3496901E-939.66
CGATTCG1500.039.610
ACTACGC452.3528628E-839.11110721
ACGGGTA1700.038.823535
CGACGGT3250.038.58461828
Produced by FastQC (version 0.11.5)