##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546742_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2667248
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.517473815708176	31.0	31.0	33.0	30.0	34.0
2	31.914274188226965	31.0	31.0	34.0	30.0	34.0
3	32.05869420466338	33.0	31.0	34.0	30.0	34.0
4	35.73514348871946	37.0	35.0	37.0	35.0	37.0
5	35.62815606197849	37.0	35.0	37.0	33.0	37.0
6	35.67898185695518	37.0	35.0	37.0	33.0	37.0
7	36.00502128036088	37.0	35.0	37.0	35.0	37.0
8	22.18169176619497	35.0	0.0	37.0	0.0	37.0
9	30.019649466416322	37.0	18.0	39.0	17.0	39.0
10	34.402557242521134	35.0	32.0	39.0	27.0	39.0
11	35.982165512918186	37.0	35.0	39.0	32.0	39.0
12	36.28529255622274	37.0	35.0	39.0	32.0	39.0
13	36.32099302352087	38.0	35.0	39.0	32.0	39.0
14	37.4073794412818	39.0	35.0	41.0	33.0	41.0
15	37.686991985747106	39.0	36.0	41.0	33.0	41.0
16	37.7731377059801	40.0	35.0	41.0	33.0	41.0
17	37.72771635783399	40.0	35.0	41.0	33.0	41.0
18	37.713251261225054	39.0	36.0	41.0	33.0	41.0
19	37.717590190338505	39.0	36.0	41.0	33.0	41.0
20	37.62143452727305	39.0	35.0	41.0	33.0	41.0
21	37.494005431815864	39.0	35.0	41.0	33.0	41.0
22	37.452718307408986	39.0	35.0	41.0	33.0	41.0
23	37.33931040533164	39.0	35.0	41.0	32.0	41.0
24	37.241175548730375	39.0	35.0	41.0	32.0	41.0
25	37.098736600421105	39.0	35.0	41.0	32.0	41.0
26	37.03635919869468	39.0	35.0	41.0	32.0	41.0
27	36.91788652573739	39.0	35.0	41.0	32.0	41.0
28	36.95360386435757	39.0	35.0	41.0	32.0	41.0
29	36.86644848922935	39.0	35.0	41.0	31.0	41.0
30	36.78162135654427	38.0	35.0	40.0	31.0	41.0
31	36.71950114874957	38.0	35.0	40.0	31.0	41.0
32	36.527717332621485	38.0	35.0	41.0	31.0	41.0
33	36.421100325129125	38.0	35.0	41.0	30.0	41.0
34	36.36655496601741	39.0	35.0	41.0	30.0	41.0
35	36.23744661163866	38.0	35.0	41.0	30.0	41.0
36	36.11635888376334	38.0	35.0	41.0	30.0	41.0
37	36.08352541645921	38.0	35.0	40.0	30.0	41.0
38	36.00455675662705	38.0	35.0	40.0	30.0	41.0
39	35.915618270217095	38.0	35.0	40.0	30.0	41.0
40	35.814619038049706	38.0	35.0	40.0	29.0	41.0
41	35.82926934428295	38.0	35.0	40.0	29.0	41.0
42	35.603467319124434	38.0	35.0	40.0	29.0	41.0
43	35.632549729159045	38.0	35.0	40.0	29.0	41.0
44	35.433585478365714	38.0	34.0	40.0	28.0	41.0
45	35.234693961716346	37.0	34.0	40.0	27.0	41.0
46	35.2992308926654	37.0	34.0	40.0	28.0	41.0
47	35.157417682945116	37.0	34.0	40.0	27.0	41.0
48	35.18422433909408	37.0	34.0	40.0	28.0	41.0
49	35.11589286035644	37.0	34.0	40.0	27.0	41.0
50	34.65963157531658	36.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	5.0
13	10.0
14	21.0
15	45.0
16	113.0
17	337.0
18	822.0
19	1679.0
20	3014.0
21	5140.0
22	7999.0
23	11890.0
24	17118.0
25	24184.0
26	31388.0
27	37544.0
28	42838.0
29	49949.0
30	61640.0
31	78388.0
32	101652.0
33	135629.0
34	217372.0
35	352260.0
36	258146.0
37	380042.0
38	444311.0
39	403707.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.274614134118764	24.58856469289695	30.2604032320954	12.876417940888887
2	30.415206984877297	26.45618255220362	29.577977001013778	13.550633461905303
3	29.4724750004499	25.846115546810793	31.048968824796198	13.632440627943106
4	26.150811623066172	27.471161286839468	30.75113375284188	15.62689333725248
5	23.46613438270457	32.02360635381487	29.348939431203995	15.161319832276563
6	21.9642118018272	40.363813188724855	26.54432583696754	11.127649172480398
7	87.24621782451426	4.684978674648926	6.081324271308855	1.9874792295279629
8	54.741891267703636	40.053530830279	3.399758852570139	1.8048190494472205
9	83.35426626995314	5.514935244116782	7.7462613150333235	3.3845371708967447
10	49.379247823974374	24.951560559797965	13.105980396273612	12.563211219954052
11	42.36565178791024	22.307374492360665	20.496256816014107	14.830716903714992
12	37.773652843680075	20.90727971302256	24.8846751408193	16.43439230247806
13	23.143104803152912	35.089163062452386	25.302896468569852	16.46483566582485
14	17.391408672909307	38.784357510062804	27.251196739110874	16.573037077917014
15	16.562014480843175	23.30424467466092	43.50326628794923	16.630474556546673
16	18.586423159751174	19.04119901861394	41.82539456398505	20.54698325764983
17	17.984341913462863	19.75114425055338	28.82435379087359	33.44016004511017
18	22.525464448750174	23.274438672369424	31.430110735859586	22.76998614302082
19	28.49382584596558	24.492060730760695	25.320667594464407	21.693445828809317
20	30.757882281662596	23.873595556168755	23.724153134616653	21.644369027551992
21	24.24997600523086	27.156904794754745	27.24838485210224	21.344734347912155
22	24.340706226042723	24.763389081180303	24.640640840296815	26.25526385248016
23	21.967848509025032	28.948451737521218	24.276707677726257	24.80699207572749
24	21.98816907914075	24.72923402698212	33.70961380419069	19.57298308968645
25	20.60052158629419	24.63025560427827	31.819950750736343	22.949272058691207
26	19.317663749302653	31.73015782559402	26.497723496277814	22.454454928825516
27	21.060958711001003	29.845668644235555	27.600358121929418	21.493014522834024
28	18.057938369435465	28.347007852288204	34.47973341811485	19.115320360161483
29	19.26903684996671	24.82228874105445	34.10792697192012	21.80074743705872
30	21.450761234050976	26.993740364600516	30.285522756039185	21.269975645309323
31	28.411868712620645	26.05010857633036	24.12272874513356	21.41529396591543
32	29.690939875107226	26.60210074203824	24.620432745661446	19.086526637193092
33	27.461263444569084	26.552011661457804	25.105764443351347	20.880960450621764
34	21.705030803284885	27.084283126278468	27.97557632436129	23.235109746075356
35	22.15170842756279	25.032205479205533	29.502693412836003	23.313392680395673
36	28.79578501886589	24.397206409002838	26.38701013179127	20.419998440340006
37	23.282124496859684	28.87496775702897	27.795128162060674	20.047779584050677
38	23.04963767898598	29.817774725109924	23.95735229719921	23.175235298704884
39	22.64142666898616	28.454796854285764	26.32233673059273	22.58143974613534
40	23.777803938741354	25.23387401546463	26.401669436062935	24.58665260973108
41	20.065475726291666	24.28934242335171	27.04382944518095	28.601352405175675
42	23.311480597229803	26.260062806308227	24.35873979472475	26.06971680173722
43	23.39669951950475	26.179342903247093	26.202775294985692	24.221182282262465
44	22.08418564752884	28.634570163704314	26.95249935514058	22.328744833626267
45	20.524525653407558	31.051743219978047	24.431886348775965	23.99184477783843
46	23.03044186367372	29.346727413423874	26.645516277451513	20.977314445450894
47	23.371579995560968	26.617434899191977	26.680458660012118	23.330526445234938
48	23.840602748600805	23.552478059782967	28.550213553445346	24.05670563817088
49	21.80517147261897	22.648700083381822	30.92996976659088	24.616158677408325
50	20.509641398175198	28.791211015998513	27.434231837459432	23.264915748366857
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1627.0
1	2770.0
2	3913.0
3	10091.0
4	16269.0
5	12156.5
6	8044.0
7	8566.0
8	9088.0
9	9772.0
10	10456.0
11	10741.5
12	11027.0
13	10826.5
14	10626.0
15	10126.5
16	9627.0
17	9270.5
18	8914.0
19	9122.0
20	9330.0
21	9238.5
22	9147.0
23	10183.0
24	11219.0
25	13964.5
26	16710.0
27	19766.0
28	22822.0
29	26040.5
30	29259.0
31	35992.0
32	42725.0
33	47563.0
34	52401.0
35	58062.5
36	63724.0
37	75511.5
38	87299.0
39	113568.0
40	139837.0
41	168720.5
42	197604.0
43	217862.0
44	238120.0
45	242574.5
46	247029.0
47	241330.0
48	235631.0
49	227314.5
50	218998.0
51	197784.5
52	176571.0
53	161391.0
54	146211.0
55	138265.0
56	130319.0
57	124939.5
58	119560.0
59	116230.5
60	112901.0
61	102301.0
62	91701.0
63	76663.0
64	61625.0
65	50835.0
66	40045.0
67	33911.0
68	27777.0
69	23566.0
70	19355.0
71	15939.0
72	12523.0
73	10255.5
74	7988.0
75	6083.0
76	4178.0
77	3391.5
78	2605.0
79	1999.0
80	1393.0
81	1019.0
82	645.0
83	442.5
84	240.0
85	156.0
86	72.0
87	50.5
88	29.0
89	35.5
90	42.0
91	29.0
92	16.0
93	10.0
94	4.0
95	3.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2667248.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.604932819256305
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.83497560884784	16.24389376228019
2	7.286966492819766	3.0029491008144604
3	2.307299512857079	1.4262525436896873
4	1.21474941269737	1.0011932016344145
5	0.8129870867109432	0.8375772152300943
6	0.6061982049254374	0.7494403972585442
7	0.4484386931205888	0.646803440171338
8	0.3599440698829609	0.5933298702902485
9	0.3227020670615467	0.5984328971194481
>10	5.05925803299235	27.036502536332247
>50	2.2128529140939417	32.0636332212561
>100	0.5297490227113562	14.709180246805817
>500	0.0031400467496297624	0.435357925971041
>1k	3.6941726466232496E-4	0.17229349863370072
>5k	3.6941726466232496E-4	0.4831601425126642
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	7145	0.26787910235568646	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5550	0.20807963863877674	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3445	0.12915934326316864	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.037641794088888625	0.0
2	0.0	0.0	0.0	0.13290852594134478	0.0
3	0.0	0.0	0.0	0.21145390304913528	0.0
4	0.0	0.0	0.0	0.33461455402722207	0.0
5	0.0	0.0	0.0	0.5737749170680791	0.0
6	0.0	0.0	0.0	0.8521142390958771	0.0
7	0.0	0.0	0.0	1.022589575472547	0.0
8	0.0	0.0	0.0	1.4454598897440358	0.0
9	0.0	0.0	0.0	1.7677396327600583	0.0
10	0.0	0.0	0.0	2.295249635579444	0.0
11	0.0	0.0	0.0	2.783430712104761	0.0
12	0.0	0.0	0.0	3.185080652417773	0.0
13	0.0	0.0	0.0	3.35101947775385	0.0
14	0.0	0.0	0.0	3.4168926174094048	0.0
15	0.0	0.0	0.0	3.5098723478281735	0.0
16	0.0	0.0	0.0	3.717652051852696	0.0
17	3.7491826781761574E-5	0.0	0.0	3.948639196655129	0.0
18	3.7491826781761574E-5	0.0	0.0	4.274143236774383	0.0
19	3.7491826781761574E-5	0.0	0.0	4.424747904956719	0.0
20	3.7491826781761574E-5	0.0	0.0	4.593348649994301	0.0
21	3.7491826781761574E-5	0.0	0.0	4.782682375242198	0.0
22	3.7491826781761574E-5	0.0	0.0	4.97152870674193	0.0
23	7.498365356352315E-5	0.0	0.0	5.183657462673137	0.0
24	7.498365356352315E-5	0.0	0.0	5.345697138023911	0.0
25	7.498365356352315E-5	0.0	0.0	5.482617289430904	0.0
26	7.498365356352315E-5	0.0	0.0	5.615488323545467	0.0
27	7.498365356352315E-5	0.0	0.0	5.74899671871532	0.0
28	7.498365356352315E-5	0.0	0.0	5.889328626359454	0.0
29	7.498365356352315E-5	0.0	0.0	6.0373088666670665	0.0
30	7.498365356352315E-5	0.0	0.0	6.222368523661841	0.0
31	7.498365356352315E-5	0.0	0.0	6.398580109536121	0.0
32	7.498365356352315E-5	0.0	0.0	6.559570013737005	0.0
33	7.498365356352315E-5	0.0	0.0	6.722097082835941	0.0
34	7.498365356352315E-5	0.0	0.0	6.88338692165108	0.0
35	7.498365356352315E-5	0.0	0.0	7.080443963216019	0.0
36	7.498365356352315E-5	0.0	0.0	7.2643038817537775	0.0
37	7.498365356352315E-5	0.0	0.0	7.44355230559738	0.0
38	1.1247548034528473E-4	0.0	0.0	7.622875713094546	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTGC	20	7.858353E-4	44.000004	27
ACGTGCG	20	7.858353E-4	44.000004	14
TCTATCG	20	7.858353E-4	44.000004	36
TAATCGC	20	7.858353E-4	44.000004	42
ATCGTTC	30	2.528981E-6	44.0	17
TATTACG	25	4.444427E-5	44.0	1
TCTAGCG	125	0.0	42.24	1
TATGCGA	120	0.0	42.166664	2
ACGTTAG	90	0.0	41.555553	1
TAATGCG	85	0.0	41.411762	1
CGTTTTT	7345	0.0	41.124573	1
TAACGCC	225	0.0	41.066666	12
CACGACG	320	0.0	40.562504	26
TACACGA	60	3.6379788E-12	40.333332	2
TACGATC	55	7.8216544E-11	40.000004	11
CGAGTTA	50	1.3496901E-9	39.6	6
CGATTCG	150	0.0	39.6	10
ACTACGC	45	2.3528628E-8	39.111107	21
ACGGGTA	170	0.0	38.82353	5
CGACGGT	325	0.0	38.584618	28
>>END_MODULE