FastQCFastQC Report
Thu 26 May 2016
SRR1546741_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546741_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3828762
Sequences flagged as poor quality0
Sequence length50
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156740.4093751452819475No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99760.26055419480239306No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA38300.10003233421142395No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTG302.5292702E-644.01
CGTTTTT230700.043.1608121
CGACGGT6650.042.34586328
TATCGCG1100.042.01
CGGTCTA6750.041.06666631
ACGGTCT6950.039.8848930
CGGTCCG501.3496901E-939.60000229
CGTAAGA2400.039.4166642
GACGGTC7150.039.07692329
GAGCGAT14650.039.044377
GCGCGAC7250.038.841389
CTAGCGA1700.038.8235322
CACGACG7450.037.7986626
GTCGTTA357.294657E-637.71428732
CTTACGA1300.037.230772
ACGGGAC7700.036.8571435
ACGCGAG2450.036.8163263
TCTCACG7950.036.8050323
GACCGAT12800.036.6093759
ACGACGG7750.036.33548427
Produced by FastQC (version 0.11.5)