##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546741_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3828762
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.50428728659551	31.0	31.0	33.0	30.0	34.0
2	31.896022265160383	31.0	31.0	34.0	30.0	34.0
3	32.01537598837431	33.0	31.0	34.0	30.0	34.0
4	35.71599018168275	37.0	35.0	37.0	35.0	37.0
5	35.60918072212376	37.0	35.0	37.0	33.0	37.0
6	35.658657028041965	37.0	35.0	37.0	33.0	37.0
7	36.04492104758666	37.0	35.0	37.0	35.0	37.0
8	21.823966598080528	35.0	0.0	37.0	0.0	37.0
9	29.8384402059987	37.0	18.0	39.0	17.0	39.0
10	34.279498438398626	35.0	32.0	39.0	27.0	39.0
11	35.88850181860351	37.0	35.0	39.0	32.0	39.0
12	36.54412496780944	38.0	35.0	39.0	32.0	39.0
13	36.77511947726184	39.0	35.0	39.0	33.0	39.0
14	37.9504845169274	40.0	37.0	41.0	33.0	41.0
15	38.15593212636356	40.0	37.0	41.0	33.0	41.0
16	38.176731016448656	40.0	37.0	41.0	33.0	41.0
17	38.122356782688506	40.0	37.0	41.0	33.0	41.0
18	38.06313450666299	40.0	37.0	41.0	33.0	41.0
19	38.075121932363515	40.0	37.0	41.0	33.0	41.0
20	37.94326756272654	40.0	36.0	41.0	33.0	41.0
21	37.867969855530326	40.0	36.0	41.0	33.0	41.0
22	37.84247022928038	40.0	36.0	41.0	33.0	41.0
23	37.686381394299254	39.0	36.0	41.0	33.0	41.0
24	37.582515183759135	39.0	35.0	41.0	33.0	41.0
25	37.46025817222382	39.0	35.0	41.0	33.0	41.0
26	37.40971650888721	39.0	35.0	41.0	33.0	41.0
27	37.299686164875226	39.0	35.0	41.0	32.0	41.0
28	37.2793224023849	39.0	35.0	41.0	32.0	41.0
29	37.17817900407495	39.0	35.0	41.0	32.0	41.0
30	37.045031527162045	39.0	35.0	41.0	31.0	41.0
31	36.96838873766507	39.0	35.0	41.0	31.0	41.0
32	36.68709441850917	39.0	35.0	41.0	30.0	41.0
33	36.50864404734481	39.0	35.0	41.0	30.0	41.0
34	36.37149449352036	39.0	35.0	41.0	30.0	41.0
35	36.19438293631205	39.0	35.0	41.0	29.0	41.0
36	36.11187950569923	39.0	35.0	41.0	29.0	41.0
37	36.11951434954693	39.0	35.0	41.0	29.0	41.0
38	36.029842544404694	39.0	35.0	41.0	28.0	41.0
39	35.94047684342876	39.0	35.0	41.0	27.0	41.0
40	35.90766832725565	39.0	35.0	41.0	27.0	41.0
41	35.89816342723836	39.0	35.0	41.0	27.0	41.0
42	35.64115241427908	39.0	35.0	41.0	26.0	41.0
43	35.68812321058347	39.0	35.0	41.0	26.0	41.0
44	35.51216607352455	39.0	35.0	40.0	26.0	41.0
45	35.30086095714489	38.0	35.0	40.0	24.0	41.0
46	35.372199943480425	38.0	35.0	40.0	25.0	41.0
47	35.250129676381036	38.0	34.0	40.0	24.0	41.0
48	35.25674382476633	38.0	34.0	40.0	24.0	41.0
49	35.15767080847543	38.0	34.0	40.0	24.0	41.0
50	34.72453236842614	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	4.0
12	4.0
13	11.0
14	26.0
15	39.0
16	122.0
17	368.0
18	949.0
19	2283.0
20	4256.0
21	7570.0
22	12306.0
23	18548.0
24	28799.0
25	42780.0
26	58025.0
27	65472.0
28	67219.0
29	72418.0
30	84552.0
31	105000.0
32	134032.0
33	175088.0
34	252774.0
35	357344.0
36	365000.0
37	562157.0
38	680015.0
39	731599.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.88329230179363	23.170648893819987	29.218112799907647	14.727946004478731
2	33.33116553079037	24.466315743835736	28.9283846841355	13.274134041238394
3	28.267022081811298	24.572799249470194	34.120088947811325	13.040089720907174
4	24.787855708973293	26.850323942830606	34.03191423232889	14.329906115867216
5	22.49983153823612	31.540351685479536	32.14866842075846	13.811148355525885
6	19.862503858949708	40.568282907112014	29.04134547929592	10.527867754642362
7	86.3541792360037	3.1273294083048255	8.881147483181248	1.6373438725102267
8	52.91749656938718	40.36336027154469	5.162556460809003	1.556586698259124
9	81.75478131051239	5.013683274123594	10.44805083209664	2.783484583267385
10	38.50257602849172	34.043667378646155	17.061676855338618	10.392079737523513
11	26.56937150964202	25.84921705762855	32.05767295015987	15.523738482569563
12	25.75069957338691	23.521467252339008	32.72914325831692	17.99868991595717
13	21.36191802990105	27.574970708547568	33.545412329102724	17.51769893244866
14	18.10008039152081	31.04954029527038	32.51599342032751	18.334385892881304
15	17.199058076736033	26.517683783948964	39.63832173428382	16.64493640503118
16	19.73452515460611	25.511771167808288	37.19810737778948	17.55559629979612
17	19.887263820524755	24.874228275353758	32.839074353537775	22.399433550583716
18	21.048109023229962	26.068374059291227	33.855146911717156	19.02837000576165
19	23.284706649303352	27.5223166130462	30.644970880927048	18.5480058567234
20	24.830898342597425	26.267315649288204	30.44757025900278	18.454215749111594
21	22.525845168751673	27.496929817000897	31.522147367739233	18.45507764650819
22	21.45918184520218	24.303835025525224	31.980781255142	22.256201874130593
23	19.63360480489516	28.189999796278798	31.530531278778884	20.645864120047158
24	18.98344686872676	26.49125226378657	34.410443898053735	20.11485696943294
25	19.469321937482665	27.768244670209324	32.553734079057406	20.2086993132506
26	19.4377451510436	29.804568683036447	31.1472742364242	19.610411929495747
27	20.334327388330745	28.327720553014263	31.69737372027825	19.640578338376738
28	18.411303706002098	28.282379526332534	34.51778930108479	18.788527466580582
29	19.755576345565487	25.398444719206886	33.976935625667	20.869043309560638
30	20.694678854418218	26.697271859676835	32.4724023065419	20.13564697936304
31	22.569488518743132	26.49039036638997	29.311171600637493	21.628949514229404
32	23.63019691482521	27.112210160882288	29.591549435561674	19.66604348873082
33	22.248784332899252	27.6488588217288	28.879047587705898	21.223309257666052
34	20.26002138550268	28.4651278925146	30.24160289931837	21.03324782266435
35	19.86485448821316	27.216186328635732	29.880154472907954	23.038804710243156
36	21.8737022567608	29.026614869244945	28.94510027000895	20.15458260398531
37	20.133009050967388	29.549655998466346	29.333633169154936	20.98370178141133
38	20.12859509157268	29.801408392582253	28.526714379217093	21.543282136627976
39	20.188118248143915	29.47255535862506	28.70982839884015	21.629497994390874
40	21.075402440788952	27.21488042348937	29.91562285668318	21.794094279038497
41	19.386423078791527	27.36098509126449	29.520638786114155	23.731953043829833
42	21.619599233381443	27.68289070984303	28.464370467529715	22.233139589245816
43	20.606660847553336	27.763386703064857	29.595911158750532	22.03404129063128
44	20.61300754656466	29.337864301829153	29.040796999134443	21.008331152471737
45	20.02558529362755	29.236552180574293	28.63792526148139	22.099937264316768
46	20.920992216282965	29.547096424379472	28.263861791357105	21.26804956798046
47	20.34736032169145	28.005057509450836	29.187789682409093	22.45979248644862
48	20.796017093776005	26.88359840595994	29.619130152252872	22.701254348011187
49	20.552700846905605	26.709207832714593	30.094610216043723	22.643481104336075
50	19.436099710559184	27.65820910257676	30.11631435957628	22.78937682728778
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	931.0
1	2267.0
2	3603.0
3	26251.5
4	48900.0
5	36753.0
6	24606.0
7	24728.5
8	24851.0
9	25098.0
10	25345.0
11	25451.0
12	25557.0
13	24728.5
14	23900.0
15	22823.5
16	21747.0
17	21160.0
18	20573.0
19	20617.0
20	20661.0
21	22027.0
22	23393.0
23	26206.5
24	29020.0
25	33841.5
26	38663.0
27	45881.5
28	53100.0
29	65774.0
30	78448.0
31	91499.0
32	104550.0
33	118090.5
34	131631.0
35	146452.0
36	161273.0
37	173403.5
38	185534.0
39	205261.5
40	224989.0
41	250312.5
42	275636.0
43	298002.0
44	320368.0
45	335764.0
46	351160.0
47	350558.0
48	349956.0
49	331291.5
50	312627.0
51	278026.5
52	243426.0
53	218599.5
54	193773.0
55	170562.0
56	147351.0
57	133578.5
58	119806.0
59	106191.5
60	92577.0
61	78854.0
62	65131.0
63	51050.0
64	36969.0
65	28081.5
66	19194.0
67	15265.0
68	11336.0
69	9492.0
70	7648.0
71	6393.0
72	5138.0
73	3958.5
74	2779.0
75	2017.5
76	1256.0
77	954.0
78	652.0
79	492.5
80	333.0
81	288.5
82	244.0
83	171.5
84	99.0
85	56.0
86	13.0
87	10.5
88	8.0
89	4.5
90	1.0
91	2.5
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3828762.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.02043541571436
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.08775040677794	21.099650166247667
2	7.7095527359769545	4.166309435730185
3	2.7446008897910903	2.22480933253537
4	1.521117409482766	1.6440501889059127
5	0.9855114258568458	1.331447391690674
6	0.7648672844847174	1.2400228237207278
7	0.599376298384694	1.1336785992149496
8	0.47657923119859713	1.0301902669657999
9	0.4190511338838353	1.0190649689091038
>10	5.347633307366528	33.434896575760305
>50	1.0398559650720627	19.30740233442518
>100	0.30179776735766944	11.113295325507853
>500	0.0017820206466998133	0.32521734182223877
>1k	3.1447423176914817E-4	0.20345202632825773
>5k	1.048247439230494E-4	0.2825612438606013
>10k+	1.048247439230494E-4	0.44395197837520695
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15674	0.4093751452819475	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9976	0.26055419480239306	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3830	0.10003233421142395	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04724764819542191	0.0
2	0.0	0.0	0.0	0.15882418390069689	0.0
3	0.0	0.0	0.0	0.24365578220845277	0.0
4	0.0	0.0	0.0	0.35343016881174644	0.0
5	0.0	0.0	0.0	0.6088390973374683	0.0
6	0.0	0.0	0.0	0.9405128864108033	0.0
7	0.0	0.0	0.0	1.1976978459355792	0.0
8	0.0	0.0	0.0	1.8921520846686213	0.0
9	0.0	0.0	0.0	2.493234105436692	0.0
10	0.0	0.0	0.0	3.3562284623593737	0.0
11	0.0	0.0	0.0	3.8691618857479257	0.0
12	0.0	0.0	0.0	4.3577793553111945	0.0
13	0.0	0.0	0.0	4.580096647428072	0.0
14	0.0	0.0	0.0	4.669211614615899	0.0
15	0.0	0.0	0.0	4.782616417526083	0.0
16	0.0	0.0	0.0	5.0223544842954455	0.0
17	0.0	0.0	0.0	5.322190305900445	0.0
18	0.0	0.0	0.0	5.750422721495878	0.0
19	0.0	0.0	0.0	5.968430526629756	0.0
20	0.0	0.0	0.0	6.196937809140396	0.0
21	0.0	0.0	0.0	6.444694133508429	0.0
22	0.0	0.0	0.0	6.724836905506271	0.0
23	0.0	0.0	0.0	7.037940723398321	0.0
24	0.0	0.0	0.0	7.264959274042106	0.0
25	0.0	0.0	0.0	7.459069014997537	0.0
26	2.611810292726474E-5	0.0	0.0	7.6543279524817684	0.0
27	2.611810292726474E-5	0.0	0.0	7.855776880359762	0.0
28	2.611810292726474E-5	0.0	0.0	8.072896669994114	0.0
29	2.611810292726474E-5	0.0	0.0	8.297904126712499	0.0
30	2.611810292726474E-5	0.0	0.0	8.576061922887869	0.0
31	2.611810292726474E-5	0.0	0.0	8.827814317003773	0.0
32	2.611810292726474E-5	0.0	0.0	9.066690486376537	0.0
33	2.611810292726474E-5	0.0	0.0	9.31167829183428	0.0
34	2.611810292726474E-5	0.0	0.0	9.559669679128659	0.0
35	2.611810292726474E-5	0.0	0.0	9.826988462589213	0.0
36	5.223620585452948E-5	0.0	0.0	10.091277546110204	0.0
37	5.223620585452948E-5	0.0	0.0	10.368286145756775	0.0
38	5.223620585452948E-5	0.0	0.0	10.637877204172002	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCGTG	30	2.5292702E-6	44.0	1
CGTTTTT	23070	0.0	43.160812	1
CGACGGT	665	0.0	42.345863	28
TATCGCG	110	0.0	42.0	1
CGGTCTA	675	0.0	41.066666	31
ACGGTCT	695	0.0	39.88489	30
CGGTCCG	50	1.3496901E-9	39.600002	29
CGTAAGA	240	0.0	39.416664	2
GACGGTC	715	0.0	39.076923	29
GAGCGAT	1465	0.0	39.04437	7
GCGCGAC	725	0.0	38.84138	9
CTAGCGA	170	0.0	38.823532	2
CACGACG	745	0.0	37.79866	26
GTCGTTA	35	7.294657E-6	37.714287	32
CTTACGA	130	0.0	37.23077	2
ACGGGAC	770	0.0	36.857143	5
ACGCGAG	245	0.0	36.816326	3
TCTCACG	795	0.0	36.80503	23
GACCGAT	1280	0.0	36.609375	9
ACGACGG	775	0.0	36.335484	27
>>END_MODULE