FastQCFastQC Report
Thu 26 May 2016
SRR1546740_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546740_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences746385
Sequences flagged as poor quality0
Sequence length50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17470.23406150981062054No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16130.21610830871467138No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA13680.18328342611386886No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGA551.8189894E-1244.0000042
TACGAGT302.526518E-644.04
CGGTCTA351.4451689E-744.031
CGTTTTT20500.040.8878061
GAGCGAT7900.040.379757
TTCACGA452.349043E-839.111112
CGAATAT452.349043E-839.1111114
CTATCTC3200.038.56
TACGCGG404.1219573E-738.52
TGTACGA404.1219573E-738.52
AATCGTT1550.038.32258222
TTAATCG1500.038.1333320
TCGATCA1500.038.1333317
TAATCGT1500.038.1333321
AGCGATA1850.038.0540548
TCGTTTA357.286775E-637.71428738
CGCATCG357.286775E-637.71428721
TACCTAC357.286775E-637.71428731
ATGCGGA357.286775E-637.7142872
ACGAGCC700.037.7142875