FastQCFastQC Report
Thu 26 May 2016
SRR1546739_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546739_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1949601
Sequences flagged as poor quality0
Sequence length50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106640.5469837161552543No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99970.5127715876222879No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGA1500.044.02
CGTTTTT109550.042.212691
TATACGA1050.041.904762
TATAGCG750.041.0666661
CTATCTC13400.040.223886
TATTACG501.3496901E-939.6000021
GAGCGAT22150.039.2325067
GTTTTTA62400.039.1698722
TAATGCG452.3523171E-839.111111
AGCGATA4800.038.9583328
ATATACG750.038.133331
ACGTTAG1100.038.01
CTATACG700.037.7142871
TAACGCC700.037.71428712
TATCTCT14350.037.5609747
AGCGATC3250.037.230778
ATGTCTC20050.037.197018
GAGACCG8250.037.0666667
TTTGCGA2200.037.02
GGTACGA900.036.6666682
Produced by FastQC (version 0.11.5)