##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546739_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1949601
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.467773662405794	31.0	31.0	33.0	30.0	34.0
2	31.843979357827575	31.0	31.0	34.0	30.0	34.0
3	31.953928521784714	31.0	31.0	34.0	30.0	34.0
4	35.656353274336645	37.0	35.0	37.0	33.0	37.0
5	35.6115451315423	37.0	35.0	37.0	33.0	37.0
6	35.640120209212036	37.0	35.0	37.0	33.0	37.0
7	35.99794881106442	37.0	35.0	37.0	35.0	37.0
8	18.325622011888587	28.0	0.0	37.0	0.0	37.0
9	27.82121521275379	27.0	17.0	39.0	17.0	39.0
10	33.49345019827134	32.0	32.0	39.0	27.0	39.0
11	35.716765122709724	37.0	34.0	39.0	32.0	39.0
12	36.515736296811504	37.0	35.0	39.0	32.0	39.0
13	36.78521400019799	39.0	35.0	39.0	33.0	39.0
14	37.905937676478416	40.0	37.0	41.0	33.0	41.0
15	38.09376123627347	40.0	37.0	41.0	33.0	41.0
16	38.14964651741561	40.0	37.0	41.0	33.0	41.0
17	38.09372584441637	40.0	37.0	41.0	33.0	41.0
18	38.05432598772774	40.0	37.0	41.0	33.0	41.0
19	38.04540416218498	40.0	37.0	41.0	33.0	41.0
20	37.963322751680984	40.0	36.0	41.0	33.0	41.0
21	37.833121751578915	40.0	36.0	41.0	33.0	41.0
22	37.829301995639106	40.0	36.0	41.0	33.0	41.0
23	37.673640914217835	39.0	36.0	41.0	33.0	41.0
24	37.50562038078561	39.0	35.0	41.0	33.0	41.0
25	37.35179608545543	39.0	35.0	41.0	32.0	41.0
26	37.335556352299776	39.0	35.0	41.0	32.0	41.0
27	37.21736909244507	39.0	35.0	41.0	32.0	41.0
28	37.201560729605696	39.0	35.0	41.0	32.0	41.0
29	37.06658234172018	39.0	35.0	41.0	32.0	41.0
30	36.93794268673436	39.0	35.0	40.0	31.0	41.0
31	36.93704301546829	39.0	35.0	41.0	31.0	41.0
32	36.72217340881544	39.0	35.0	41.0	31.0	41.0
33	36.566319980344694	39.0	35.0	41.0	30.0	41.0
34	36.36504289852129	39.0	35.0	41.0	30.0	41.0
35	36.164909127559945	39.0	35.0	41.0	30.0	41.0
36	36.01279389988002	39.0	35.0	41.0	29.0	41.0
37	35.94805603813293	39.0	35.0	41.0	29.0	41.0
38	35.812516509788416	39.0	35.0	40.0	28.0	41.0
39	35.71617218087188	39.0	35.0	40.0	27.0	41.0
40	35.593541960637076	38.0	35.0	40.0	27.0	41.0
41	35.541793936297736	38.0	35.0	40.0	27.0	41.0
42	35.34741621490756	38.0	35.0	40.0	26.0	41.0
43	35.331934585589565	38.0	35.0	40.0	26.0	41.0
44	35.06292723485472	38.0	34.0	40.0	25.0	41.0
45	34.9531904220402	38.0	34.0	40.0	25.0	41.0
46	34.95494565298233	38.0	34.0	40.0	25.0	41.0
47	34.81623111600784	37.0	34.0	40.0	24.0	41.0
48	34.76363779050175	37.0	34.0	40.0	24.0	41.0
49	34.71423691309145	37.0	34.0	40.0	24.0	41.0
50	34.08574780172969	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	6.0
13	5.0
14	9.0
15	37.0
16	93.0
17	234.0
18	568.0
19	1214.0
20	2350.0
21	4024.0
22	6137.0
23	9361.0
24	14168.0
25	20483.0
26	28009.0
27	32836.0
28	35618.0
29	39263.0
30	47158.0
31	59215.0
32	75854.0
33	99780.0
34	145177.0
35	201754.0
36	221605.0
37	316263.0
38	319375.0
39	269002.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.84292529599646	24.089647061116608	27.28035121032457	15.787076432562355
2	33.12872736524037	25.230136833126366	27.82651424573541	13.81462155589785
3	29.191614078983342	24.62067879530222	32.58969399379668	13.59801313191776
4	24.97023749987818	27.596467174565465	32.165658511664695	15.267636813891663
5	23.109805544826862	30.75249756232173	30.927302560882968	15.210394331968438
6	20.795075505193115	38.78768014583497	28.85949484022628	11.557749508745635
7	83.42968638198278	3.898849046548499	10.146896723996345	2.52456784747238
8	42.2155097376335	50.78008269384351	4.929008550980432	2.075399017542564
9	76.63834805172955	5.186394549448837	13.37232592720254	4.802931471619065
10	40.230847234895755	24.65735296606844	19.071594649366716	16.040205149669088
11	31.62770228369805	24.389964921027428	27.08205422545434	16.900278569820184
12	27.162173183128242	21.163355989251134	31.06533080358494	20.60914002403569
13	23.847187193687326	23.353547725919306	33.589539603231636	19.209725477161737
14	20.728241317069493	25.740600256154977	33.30107032156836	20.230088105207166
15	19.733371084647576	22.080261550953246	38.08040722178538	20.105960142613796
16	22.862575470570647	21.371706313240505	35.21602625357701	20.54969196261184
17	22.211314007327655	23.131194536728284	31.50634411861709	23.15114733732697
18	24.35447047883131	21.868320748707042	32.64447443348665	21.132734338975002
19	26.224699310269127	25.523735369442257	28.07630894731794	20.175256372970672
20	27.44864205547699	24.217878427432073	27.284454614046673	21.049024903044263
21	25.17427924995935	26.87165219960392	28.681612288873463	19.272456261563264
22	24.2124414175003	22.673972776993857	28.976698309038618	24.136887496467228
23	22.90443018853601	26.11072727188794	27.965004121356113	23.019838418219933
24	22.888375621473315	23.90719947312296	32.0967213291335	21.10770357627022
25	23.674331311894075	24.30374215031691	29.155658003868485	22.866268533920532
26	21.07015743221305	25.32236083178045	29.89627108316009	23.711210652846404
27	22.35913912641612	23.81861724527224	30.020347753206938	23.8018958751047
28	21.971726522503836	25.235419965418565	30.84543965662718	21.947413855450424
29	23.00014208035388	24.98854893898803	29.089900959221914	22.921408021436182
30	24.259681852850917	24.888220717982808	29.727569897635465	21.12452753153081
31	25.798201785903885	25.003116022201468	26.94053808958859	22.258144102306062
32	26.341338561069676	25.42730538197303	27.22074927126115	21.01060678569615
33	26.072206569446777	26.033121648993816	25.54132871290074	22.353343068658667
34	22.18864270176308	27.756089579354953	29.13847500078221	20.916792718099757
35	23.37416732962283	26.92463740016547	27.97916086419734	21.72203440601436
36	24.942436939660986	29.150374871576286	25.687204715221217	20.219983473541507
37	24.613908179160763	28.048816142379902	25.41253312857349	21.92474254988585
38	23.854265565107937	29.691254774694926	25.199361305210655	21.255118354986482
39	24.590570070491346	26.43781984108543	24.966595729074818	24.0050143593484
40	25.130783170505143	24.98008566881121	27.375960517049386	22.51317064363426
41	22.33487775190924	24.481675994216253	27.522605907567755	25.660840346306756
42	24.175151736175764	25.564359066290997	26.768554181086284	23.491935016446956
43	23.307794774417946	24.022864165539513	28.52122049588608	24.14812056415646
44	23.949926164379278	24.77973698207992	28.03070987345616	23.239626980084644
45	22.558615839856465	24.98757438060403	28.209926031018657	24.24388374852085
46	24.152942063529924	24.939102924136787	27.34359492019136	23.564360092141932
47	22.761836909193214	25.12478194256158	28.595492103256	23.517889044989204
48	22.876373165586187	24.543073172408096	29.073230881600903	23.50732278040481
49	24.317898893158137	23.018453519463726	28.543481461078446	24.12016612629969
50	22.06318113296003	24.265324032968795	29.702333964744582	23.969160869326593
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	805.0
1	1529.5
2	2254.0
3	17472.5
4	32691.0
5	22110.0
6	11529.0
7	11452.0
8	11375.0
9	11665.0
10	11955.0
11	11912.5
12	11870.0
13	11566.5
14	11263.0
15	10922.5
16	10582.0
17	10140.5
18	9699.0
19	9563.5
20	9428.0
21	9698.0
22	9968.0
23	10267.5
24	10567.0
25	11933.5
26	13300.0
27	15606.0
28	17912.0
29	20615.5
30	23319.0
31	26914.5
32	30510.0
33	34038.5
34	37567.0
35	42255.0
36	46943.0
37	53797.0
38	60651.0
39	70519.5
40	80388.0
41	94216.0
42	108044.0
43	126026.0
44	144008.0
45	156964.5
46	169921.0
47	166555.0
48	163189.0
49	149052.5
50	134916.0
51	125134.0
52	115352.0
53	110644.0
54	105936.0
55	103272.0
56	100608.0
57	98807.0
58	97006.0
59	92315.0
60	87624.0
61	81964.0
62	76304.0
63	67953.5
64	59603.0
65	51690.0
66	43777.0
67	37289.0
68	30801.0
69	26159.5
70	21518.0
71	18393.5
72	15269.0
73	12436.0
74	9603.0
75	7498.5
76	5394.0
77	4282.0
78	3170.0
79	2458.0
80	1746.0
81	1208.0
82	670.0
83	511.5
84	353.0
85	240.0
86	127.0
87	90.0
88	53.0
89	37.5
90	22.0
91	14.5
92	7.0
93	4.5
94	2.0
95	1.0
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1949601.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.88941107536686
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.53736677054502	26.03323374352746
2	7.396019643245208	5.308775785958308
3	3.6177464859788477	3.895163724052763
4	2.5173222248293614	3.6138084854423207
5	1.9111118092882438	3.4294338667266944
6	1.5987551151342376	3.4427027721538876
7	1.3342787806170269	3.35205357526926
8	1.1702561278831278	3.3599842589651727
9	0.9913267030863574	3.202031840134899
>10	6.8404325923207985	39.304366379676736
>50	0.05654041394908119	1.3747652689079126
>100	0.02555158683499651	1.7064862426197025
>500	0.002664746994901023	0.69611902509344
>1k	3.1349964645894385E-4	0.11875765815195184
>5k	1.5674982322947192E-4	0.5623971144221045
>10k+	1.5674982322947192E-4	0.5999202588974014
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10664	0.5469837161552543	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9997	0.5127715876222879	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05416492913165309	0.0
2	0.0	0.0	0.0	0.15526253833476697	0.0
3	0.0	0.0	0.0	0.26574668355217296	0.0
4	0.0	0.0	0.0	0.4380383473336339	0.0
5	0.0	0.0	0.0	0.7195831352158724	0.0
6	0.0	0.0	0.0	1.4074161841320352	0.0
7	0.0	0.0	0.0	1.791802527799278	0.0
8	0.0	0.0	0.0	2.7792866335214232	0.0
9	0.0	0.0	0.0	3.8916167974883065	0.0
10	0.0	0.0	0.0	5.357865532485878	0.0
11	0.0	0.0	0.0	6.180905734045068	0.0
12	0.0	0.0	0.0	6.889666141943915	0.0
13	0.0	0.0	0.0	7.284157117276817	0.0
14	5.129254652618664E-5	0.0	0.0	7.4954824089647065	0.0
15	5.129254652618664E-5	0.0	0.0	7.660952164058184	0.0
16	5.129254652618664E-5	0.0	0.0	7.914901561909335	0.0
17	5.129254652618664E-5	0.0	0.0	8.18059695291498	0.0
18	5.129254652618664E-5	0.0	0.0	8.591963176054998	0.0
19	5.129254652618664E-5	0.0	0.0	8.781078795097049	0.0
20	5.129254652618664E-5	0.0	0.0	8.994660958832089	0.0
21	5.129254652618664E-5	0.0	0.0	9.22034816354731	0.0
22	5.129254652618664E-5	0.0	0.0	9.449061628507577	0.0
23	5.129254652618664E-5	0.0	0.0	9.713525998396594	0.0
24	5.129254652618664E-5	0.0	0.0	9.925620678282376	0.0
25	5.129254652618664E-5	0.0	0.0	10.11053030850928	0.0
26	5.129254652618664E-5	0.0	0.0	10.283693945581685	0.0
27	5.129254652618664E-5	0.0	0.0	10.485735286348335	0.0
28	5.129254652618664E-5	0.0	0.0	10.671619474959236	0.0
29	5.129254652618664E-5	0.0	0.0	10.865043667909486	0.0
30	5.129254652618664E-5	0.0	0.0	11.119352113586318	0.0
31	5.129254652618664E-5	0.0	0.0	11.313135354362252	0.0
32	5.129254652618664E-5	0.0	0.0	11.549696578941024	0.0
33	5.129254652618664E-5	0.0	0.0	11.766766635839847	0.0
34	5.129254652618664E-5	0.0	0.0	11.983323767273406	0.0
35	5.129254652618664E-5	0.0	0.0	12.206241174476213	0.0
36	5.129254652618664E-5	0.0	0.0	12.40941095126644	0.0
37	5.129254652618664E-5	0.0	0.0	12.636329177098288	0.0
38	5.129254652618664E-5	0.0	0.0	12.902947833941406	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTAGA	150	0.0	44.0	2
CGTTTTT	10955	0.0	42.21269	1
TATACGA	105	0.0	41.90476	2
TATAGCG	75	0.0	41.066666	1
CTATCTC	1340	0.0	40.22388	6
TATTACG	50	1.3496901E-9	39.600002	1
GAGCGAT	2215	0.0	39.232506	7
GTTTTTA	6240	0.0	39.169872	2
TAATGCG	45	2.3523171E-8	39.11111	1
AGCGATA	480	0.0	38.958332	8
ATATACG	75	0.0	38.13333	1
ACGTTAG	110	0.0	38.0	1
CTATACG	70	0.0	37.714287	1
TAACGCC	70	0.0	37.714287	12
TATCTCT	1435	0.0	37.560974	7
AGCGATC	325	0.0	37.23077	8
ATGTCTC	2005	0.0	37.19701	8
GAGACCG	825	0.0	37.066666	7
TTTGCGA	220	0.0	37.0	2
GGTACGA	90	0.0	36.666668	2
>>END_MODULE