FastQCFastQC Report
Thu 26 May 2016
SRR1546738_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546738_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences956922
Sequences flagged as poor quality0
Sequence length50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35370.36962260246916673No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32330.3378540779708273No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA10020.10471072877413207No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAG454.802132E-1044.01
ATATACG207.85582E-444.01
CCGAATC207.85582E-444.027
GTAACGA351.445751E-744.02
TACCGAA207.85582E-444.012
GCGTAAG650.043.9999961
CGTTTTT40750.041.840491
GAGCGAT10400.041.038467
GTATGCG501.3478711E-939.6000021
CTATCTC4750.039.3684236
TATCTCT4750.039.3684237
GTTTTTA23300.039.1845472
AACCGAT452.3503162E-839.111118
TACGTAG452.3503162E-839.111111
AACGTAG850.038.8235321
CGCATGA404.1235762E-738.52
ACCCGCT4350.038.43678334
AGCGATA2350.038.382988
GGTACGA357.288927E-637.7142872
ATGTCTC6950.037.3525168
Produced by FastQC (version 0.11.5)