##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546738_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	956922
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.465870781526604	31.0	31.0	33.0	30.0	34.0
2	31.848611485575628	31.0	31.0	34.0	30.0	34.0
3	31.959791916164537	31.0	31.0	34.0	30.0	34.0
4	35.66065572742606	37.0	35.0	37.0	33.0	37.0
5	35.60486852637937	37.0	35.0	37.0	33.0	37.0
6	35.63918898301011	37.0	35.0	37.0	33.0	37.0
7	35.992775795728384	37.0	35.0	37.0	35.0	37.0
8	18.652064640587216	32.0	0.0	37.0	0.0	37.0
9	28.005557401752704	31.0	17.0	39.0	17.0	39.0
10	33.58642188182527	32.0	32.0	39.0	27.0	39.0
11	35.730027107747546	37.0	35.0	39.0	32.0	39.0
12	36.43324429786336	37.0	35.0	39.0	32.0	39.0
13	36.662123976666855	39.0	35.0	39.0	33.0	39.0
14	37.79123063321776	39.0	36.0	41.0	33.0	41.0
15	37.993469687184536	40.0	37.0	41.0	33.0	41.0
16	38.046904554394196	40.0	37.0	41.0	33.0	41.0
17	37.98950071165675	40.0	36.0	41.0	33.0	41.0
18	37.940951300105965	40.0	36.0	41.0	33.0	41.0
19	37.94865830234857	40.0	36.0	41.0	33.0	41.0
20	37.88277309958387	40.0	36.0	41.0	33.0	41.0
21	37.75148444700822	40.0	36.0	41.0	33.0	41.0
22	37.73670999308199	40.0	36.0	41.0	33.0	41.0
23	37.58357629984471	39.0	36.0	41.0	33.0	41.0
24	37.44864471712428	39.0	35.0	41.0	32.0	41.0
25	37.287839552231006	39.0	35.0	41.0	32.0	41.0
26	37.27292297595833	39.0	35.0	41.0	32.0	41.0
27	37.14590217384489	39.0	35.0	41.0	32.0	41.0
28	37.1285590675102	39.0	35.0	41.0	32.0	41.0
29	37.016399455755014	39.0	35.0	41.0	32.0	41.0
30	36.8833290487626	39.0	35.0	40.0	31.0	41.0
31	36.893119815408156	39.0	35.0	41.0	31.0	41.0
32	36.6930230468105	39.0	35.0	41.0	31.0	41.0
33	36.5650962147385	39.0	35.0	41.0	30.0	41.0
34	36.40802176143928	39.0	35.0	41.0	30.0	41.0
35	36.16917157302267	39.0	35.0	41.0	30.0	41.0
36	36.064646857319616	39.0	35.0	41.0	30.0	41.0
37	35.96068018082978	39.0	35.0	40.0	29.0	41.0
38	35.87625219192369	38.0	35.0	40.0	29.0	41.0
39	35.76297441170754	38.0	35.0	40.0	28.0	41.0
40	35.70028069163422	38.0	35.0	40.0	28.0	41.0
41	35.681794336424495	38.0	35.0	40.0	28.0	41.0
42	35.51837140331187	38.0	35.0	40.0	27.0	41.0
43	35.50996528452685	38.0	35.0	40.0	28.0	41.0
44	35.25470205513093	38.0	34.0	40.0	27.0	41.0
45	35.12583993261729	38.0	34.0	40.0	26.0	41.0
46	35.10727520111357	38.0	34.0	40.0	26.0	41.0
47	34.9555648213752	37.0	34.0	40.0	26.0	41.0
48	34.91421766873371	37.0	34.0	40.0	26.0	41.0
49	34.859378298335706	37.0	34.0	40.0	26.0	41.0
50	34.21262234539492	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	2.0
11	2.0
12	2.0
13	2.0
14	9.0
15	22.0
16	42.0
17	129.0
18	283.0
19	638.0
20	1094.0
21	1857.0
22	2998.0
23	4438.0
24	6376.0
25	9479.0
26	12450.0
27	14942.0
28	16135.0
29	19095.0
30	23217.0
31	29572.0
32	37876.0
33	49985.0
34	73912.0
35	105877.0
36	107367.0
37	155195.0
38	156784.0
39	127141.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.490817433395826	23.909890252288065	27.89746708718161	14.7018252271345
2	32.961725198083016	25.208219687707047	28.127579886343923	13.702475227866012
3	29.606383801396564	25.168822537260088	31.55429596142632	13.670497699917025
4	25.321395056232376	28.497202488813087	30.890501002171543	15.290901452782984
5	23.861610455188615	31.137229575660296	30.432887946980003	14.568272022171087
6	21.559855453213533	39.795511023887	27.400247878092465	11.244385644806995
7	85.15699294195346	4.169827843857702	8.339655687715403	2.333523526473422
8	44.084470834613484	49.908351986891304	4.040036701005933	1.9671404774892833
9	79.06161630728523	5.064362612626735	11.496652809737888	4.377368270350143
10	44.22847421210924	24.047832529715066	16.601353088339486	15.122340169836205
11	35.2532390309764	23.709351441392297	24.640984322651168	16.396425204980133
12	30.49297643904101	21.15585178311294	28.68938116168298	19.66179061616307
13	24.13791301694391	25.50395957037251	31.207768240253646	19.150359172429937
14	20.483696685832285	27.729428312861444	31.594842630851833	20.192032370454434
15	20.17959666514094	21.595595043274166	38.765855524274706	19.45895276731019
16	22.79433433445986	20.470842973617493	36.132307544397555	20.602515147525086
17	22.867067535285006	22.036383320688625	30.032123830364437	25.064425313661932
18	24.896386539341762	21.92174492800876	31.900719180873676	21.281149351775795
19	27.65282854819933	25.85874292784574	26.364426776686084	20.124001747268846
20	29.165804527432748	23.945002831996756	26.047473043779952	20.841719596790544
21	26.004627336397323	27.295746152768984	26.93970877459187	19.759917736241825
22	25.012487956176155	23.275355776123863	27.41623664206696	24.29591962563302
23	23.554897891364188	26.922570491638815	26.37215990436002	23.150371712636975
24	24.133419442755	23.349029492476923	31.43641801526143	21.081133049506647
25	23.649576454507265	23.72199615015644	29.054928196864534	23.57349919847177
26	21.188142816237896	26.236203159714165	28.413914613730274	24.161739410317665
27	22.60654473405356	24.46322688787592	28.465851971216043	24.464376406854477
28	21.796551861071226	25.685583568984722	30.504889635727885	22.012974934216164
29	23.122051745074312	24.00624084303632	29.311584434259007	23.56012297763036
30	25.13945755244419	24.154006282643724	28.03467785253135	22.67185831238074
31	28.094452839416377	23.7316103088862	25.244063779492997	22.929873072204423
32	29.57827283728454	23.39605526887249	24.746949072129183	22.278722821713785
33	28.61476692980201	24.235831133572013	24.644537381312166	22.50486455531381
34	25.796250896102297	24.55142634404894	27.505585617218543	22.146737142630222
35	24.48276871051141	25.867625574498238	28.230827590963525	21.418778124026826
36	28.282869450174626	26.287931513749292	24.84340416460276	20.58579487147333
37	24.88865341166783	28.040425447424138	26.60718428461254	20.463736856295498
38	25.061290261902226	27.9272500788988	26.109338065171457	20.902121594027516
39	23.50881263049653	27.748552128595644	26.312175914024337	22.430459326883486
40	25.127962362658607	23.927551043867734	28.015449535071824	22.929037058401835
41	21.47646307640539	24.224753950687724	27.917635920169044	26.38114705273784
42	23.869448084587876	25.38137904656806	25.869611107279383	24.879561761564684
43	22.77750955668278	24.398226814724712	27.95410702230694	24.87015660628557
44	23.645605388944972	25.237689174248267	27.550625860832962	23.5660795759738
45	22.315611930752976	26.194506971310094	27.321140072022587	24.168741025914336
46	24.583090366821956	25.63834878913851	26.751814672460245	23.026746171579294
47	23.250170860320903	24.77276099828408	27.728174292157565	24.24889384923745
48	23.666296730559022	23.3885311446492	28.893682034690393	24.05149009010139
49	24.35977017980567	22.638522262002546	28.540570704822336	24.46113685336945
50	22.88995341313085	24.603677206710685	28.6449679284205	23.86140145173797
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	401.0
1	772.0
2	1143.0
3	6254.5
4	11366.0
5	7846.5
6	4327.0
7	4352.0
8	4377.0
9	4559.5
10	4742.0
11	4732.5
12	4723.0
13	4571.5
14	4420.0
15	4314.5
16	4209.0
17	4133.5
18	4058.0
19	4042.5
20	4027.0
21	4108.5
22	4190.0
23	4449.0
24	4708.0
25	5529.5
26	6351.0
27	7404.5
28	8458.0
29	10032.0
30	11606.0
31	13069.0
32	14532.0
33	16421.0
34	18310.0
35	20831.0
36	23352.0
37	26122.0
38	28892.0
39	33982.0
40	39072.0
41	44347.5
42	49623.0
43	54771.0
44	59919.0
45	66048.0
46	72177.0
47	76419.5
48	80662.0
49	78756.0
50	76850.0
51	70096.0
52	63342.0
53	59884.5
54	56427.0
55	54495.0
56	52563.0
57	51680.0
58	50797.0
59	48429.5
60	46062.0
61	43033.0
62	40004.0
63	35722.5
64	31441.0
65	27130.5
66	22820.0
67	19572.0
68	16324.0
69	13867.5
70	11411.0
71	9596.0
72	7781.0
73	6361.0
74	4941.0
75	4033.5
76	3126.0
77	2472.0
78	1818.0
79	1356.5
80	895.0
81	651.5
82	408.0
83	273.5
84	139.0
85	102.5
86	66.0
87	51.5
88	37.0
89	28.5
90	20.0
91	11.5
92	3.0
93	2.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	956922.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.92358597767566
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.71855851332751	24.68617045015456
2	7.869200603867571	5.653798089372277
3	4.252869072794631	4.583349233650071
4	3.18118542807867	4.571183529460521
5	2.4585964090617973	4.416079974266805
6	2.0636811732065503	4.448089683371765
7	1.7259905900816017	4.340263995161889
8	1.48532569412627	4.268658022223667
9	1.2722192132832897	4.1132408661749835
>10	6.9271137897678985	36.36006239827278
>50	0.03241194142893866	0.7879792346834404
>100	0.011679609978020845	0.9017682871186208
>500	2.9199024930061867E-4	0.054124978019031614
>1k	8.759707479018561E-4	0.8152312580696002
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3537	0.36962260246916673	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3233	0.3378540779708273	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1002	0.10471072877413207	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.044099728086510706	0.0
2	0.0	0.0	0.0	0.1324036859848556	0.0
3	0.0	0.0	0.0	0.21328802138523306	0.0
4	0.0	0.0	0.0	0.36188947479522887	0.0
5	0.0	0.0	0.0	0.5801935789959892	0.0
6	0.0	0.0	0.0	1.174494890910649	0.0
7	0.0	0.0	0.0	1.4826704788896066	0.0
8	0.0	0.0	0.0	2.400091125504482	0.0
9	0.0	0.0	0.0	3.3527288535533724	0.0
10	0.0	0.0	0.0	4.564948867305799	0.0
11	0.0	0.0	0.0	5.224459255822314	0.0
12	0.0	0.0	0.0	5.799741253728099	0.0
13	0.0	0.0	0.0	6.106662821003175	0.0
14	0.0	0.0	0.0	6.262161388284521	0.0
15	0.0	0.0	0.0	6.389548991453849	0.0
16	0.0	0.0	0.0	6.5848627160834425	0.0
17	0.0	0.0	0.0	6.786028537331151	0.0
18	0.0	0.0	0.0	7.079469382039497	0.0
19	0.0	0.0	0.0	7.2245177767884945	0.0
20	0.0	0.0	0.0	7.396632118396274	0.0
21	0.0	0.0	0.0	7.595498901686867	0.0
22	0.0	0.0	0.0	7.782974996917199	0.0
23	0.0	0.0	0.0	7.9943819872466095	0.0
24	0.0	0.0	0.0	8.176319491034796	0.0
25	0.0	0.0	0.0	8.321994896135735	0.0
26	0.0	0.0	0.0	8.461609201167912	0.0
27	0.0	0.0	0.0	8.623691377144636	0.0
28	0.0	0.0	0.0	8.770202796048162	0.0
29	1.0450172532348509E-4	0.0	0.0	8.929254421990507	0.0
30	1.0450172532348509E-4	0.0	0.0	9.148499041719178	0.0
31	1.0450172532348509E-4	0.0	0.0	9.305356131429729	0.0
32	1.0450172532348509E-4	0.0	0.0	9.483009064479655	0.0
33	1.0450172532348509E-4	0.0	0.0	9.65794495267117	0.0
34	1.0450172532348509E-4	0.0	0.0	9.840613968536621	0.0
35	1.0450172532348509E-4	0.0	0.0	10.031852125878599	0.0
36	1.0450172532348509E-4	0.0	0.0	10.207415024422053	0.0
37	1.0450172532348509E-4	0.0	0.0	10.396458645532238	0.0
38	1.0450172532348509E-4	0.0	0.0	10.620823849801761	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATAG	45	4.802132E-10	44.0	1
ATATACG	20	7.85582E-4	44.0	1
CCGAATC	20	7.85582E-4	44.0	27
GTAACGA	35	1.445751E-7	44.0	2
TACCGAA	20	7.85582E-4	44.0	12
GCGTAAG	65	0.0	43.999996	1
CGTTTTT	4075	0.0	41.84049	1
GAGCGAT	1040	0.0	41.03846	7
GTATGCG	50	1.3478711E-9	39.600002	1
CTATCTC	475	0.0	39.368423	6
TATCTCT	475	0.0	39.368423	7
GTTTTTA	2330	0.0	39.184547	2
AACCGAT	45	2.3503162E-8	39.11111	8
TACGTAG	45	2.3503162E-8	39.11111	1
AACGTAG	85	0.0	38.823532	1
CGCATGA	40	4.1235762E-7	38.5	2
ACCCGCT	435	0.0	38.436783	34
AGCGATA	235	0.0	38.38298	8
GGTACGA	35	7.288927E-6	37.714287	2
ATGTCTC	695	0.0	37.352516	8
>>END_MODULE