FastQCFastQC Report
Thu 26 May 2016
SRR1546737_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546737_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1449595
Sequences flagged as poor quality0
Sequence length50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60200.41528840814158435No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55120.38024413715555033No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGA502.7284841E-1144.02
TACGAGT502.7284841E-1144.04
TCATACG302.528177E-644.01
AATCGTA207.857164E-444.012
CGTTTTT63800.041.6896551
CTATCTC9350.040.9411776
ATACGAA2300.040.17391619
GTTACGA557.8216544E-1140.02
GCTAACG557.8216544E-1140.01
GAGCGAT14300.039.6923077
AACCGAT501.3478711E-939.6000028
AATACGG501.3478711E-939.6000022
GTTTTTA33550.039.344262
TACGAAA2350.039.31915320
TATCTCT9650.039.2124377
AGCGATA2750.039.28
ATTAACG452.3515895E-839.111111
GCTATCT5900.038.779665
TAGCATA6850.038.21897530
ATGTCTC12550.038.2151378
Produced by FastQC (version 0.11.5)