##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546737_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1449595
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.530828955673826	31.0	31.0	33.0	30.0	34.0
2	31.912065783891364	31.0	31.0	34.0	30.0	34.0
3	32.01526219392313	33.0	31.0	34.0	30.0	34.0
4	35.708384065894265	37.0	35.0	37.0	35.0	37.0
5	35.651968308389584	37.0	35.0	37.0	33.0	37.0
6	35.68747132819857	37.0	35.0	37.0	33.0	37.0
7	36.028404485390745	37.0	35.0	37.0	35.0	37.0
8	18.701885699109063	32.0	0.0	37.0	0.0	37.0
9	28.053988872754115	32.0	17.0	39.0	17.0	39.0
10	33.68271482724485	32.0	32.0	39.0	27.0	39.0
11	35.8284727803283	37.0	35.0	39.0	32.0	39.0
12	36.53728386204423	37.0	35.0	39.0	33.0	39.0
13	36.7312663192133	39.0	35.0	39.0	33.0	39.0
14	37.81504144260983	40.0	36.0	41.0	33.0	41.0
15	38.056480603202964	40.0	37.0	41.0	33.0	41.0
16	38.129621721929226	40.0	37.0	41.0	33.0	41.0
17	38.10477823116112	40.0	37.0	41.0	33.0	41.0
18	38.09101300708129	40.0	37.0	41.0	33.0	41.0
19	38.08411659808429	40.0	37.0	41.0	34.0	41.0
20	37.9850151249142	40.0	36.0	41.0	33.0	41.0
21	37.86893994529507	40.0	36.0	41.0	33.0	41.0
22	37.850821781256144	40.0	36.0	41.0	33.0	41.0
23	37.72183333965694	39.0	36.0	41.0	33.0	41.0
24	37.56582976624505	39.0	35.0	41.0	33.0	41.0
25	37.412993974178995	39.0	35.0	41.0	33.0	41.0
26	37.39114925203246	39.0	35.0	41.0	33.0	41.0
27	37.28030380899493	39.0	35.0	41.0	32.0	41.0
28	37.279841610932706	39.0	35.0	41.0	33.0	41.0
29	37.16583942411501	39.0	35.0	41.0	32.0	41.0
30	37.041409497135405	39.0	35.0	41.0	32.0	41.0
31	37.05102045743811	39.0	35.0	41.0	32.0	41.0
32	36.863589485339006	39.0	35.0	41.0	31.0	41.0
33	36.74114494048337	39.0	35.0	41.0	31.0	41.0
34	36.56650788668559	39.0	35.0	41.0	31.0	41.0
35	36.38692738316564	39.0	35.0	41.0	30.0	41.0
36	36.210860274766404	39.0	35.0	41.0	30.0	41.0
37	36.141026286652476	39.0	35.0	41.0	30.0	41.0
38	36.04693448859854	39.0	35.0	40.0	30.0	41.0
39	35.96533583518155	38.0	35.0	40.0	29.0	41.0
40	35.869947123161985	38.0	35.0	40.0	29.0	41.0
41	35.833032674643604	38.0	35.0	40.0	29.0	41.0
42	35.68972920022489	38.0	35.0	40.0	29.0	41.0
43	35.688679251791015	38.0	35.0	40.0	29.0	41.0
44	35.447461532359036	38.0	35.0	40.0	28.0	41.0
45	35.34254878086638	38.0	35.0	40.0	27.0	41.0
46	35.32657811319714	38.0	34.0	40.0	27.0	41.0
47	35.209146692696926	38.0	34.0	40.0	27.0	41.0
48	35.16085872260873	37.0	34.0	40.0	27.0	41.0
49	35.128469676012955	37.0	34.0	40.0	27.0	41.0
50	34.50710163873358	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	2.0
13	5.0
14	9.0
15	27.0
16	55.0
17	137.0
18	361.0
19	764.0
20	1423.0
21	2524.0
22	4203.0
23	6275.0
24	9350.0
25	13828.0
26	17952.0
27	20542.0
28	22158.0
29	25486.0
30	31620.0
31	41283.0
32	54356.0
33	74412.0
34	112665.0
35	164174.0
36	164760.0
37	236140.0
38	242861.0
39	202221.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.985137227984367	25.52464653920578	28.859509035282265	14.630707197527585
2	32.009630276042614	25.882677575460733	28.725264642883015	13.382427505613636
3	29.026866124676204	24.90468027276584	32.43285193450585	13.63560166805211
4	25.554654920857207	28.02596587322666	31.272458859198604	15.146920346717533
5	23.383910678499856	31.480241032840205	30.73837865058861	14.397469638071323
6	21.487518927700496	39.48447669866411	27.4069653937824	11.621038979852994
7	84.68399794425339	4.141846515750951	8.771415464319345	2.4027400756763098
8	43.74145882125697	49.90007553834002	4.155574488046661	2.2028911523563477
9	77.99813051231551	5.490843994357045	11.847102121627074	4.663923371700371
10	43.68565012986386	25.812589033488663	16.28254788406417	14.219212952583307
11	35.030611998523725	23.101280012693202	25.774302477588567	16.093805511194507
12	30.92836274959558	20.96599395003432	28.50313363387705	19.602509666493052
13	24.181719721715375	27.28168902348587	30.431603309890004	18.10498794490875
14	19.491099238063043	29.55128846332941	31.24927997130233	19.708332327305214
15	18.448394206657724	22.60886661446818	39.80980894663682	19.132930232237282
16	21.319403005667102	20.7468982715862	37.02254767710981	20.911151045636885
17	21.879421493589586	22.458479782284016	29.93125666134334	25.730842062783054
18	23.50366826596394	22.659363477385064	32.450512039569674	21.386456217081324
19	26.886475187897307	26.323766293344004	26.421655703834517	20.368102814924168
20	27.934285093422645	24.594869601509387	26.063624667579568	21.407220637488404
21	24.26105222493179	27.851641320506765	27.723467589223194	20.16383886533825
22	23.442616730880005	24.051752386011266	27.414967628889446	25.09066325421928
23	22.466964910888905	27.471673122492835	26.016439074362154	24.044922892256114
24	21.908050179532903	24.628603161572716	32.561094650574816	20.902252008319564
25	22.71227480779114	24.79989238373477	29.47002438612164	23.017808422352452
26	20.25903786919795	27.151790672567166	28.656072903121217	23.93309855511367
27	21.83416747436353	25.413581034702798	29.06963669162766	23.682614799306013
28	21.156943836036962	26.321144871498593	31.05246637854021	21.469444913924235
29	22.136941697508615	23.650467889307013	30.25776165066795	23.954828762516428
30	23.780228270654906	25.359152039017797	29.93525777889686	20.925361911430436
31	26.86019198465778	24.23249252377388	26.685936416723294	22.221379074845043
32	29.057633338967094	23.46000089680221	26.645235393333998	20.8371303708967
33	27.18690392833861	24.17482124317482	27.352605382882807	21.285669445603773
34	23.253460449297908	24.073275639057805	30.94567793073238	21.727585980911908
35	23.741320851686158	25.82369558393896	29.129791424501327	21.30519213987355
36	27.39779041732346	25.29147796453492	26.672070474856767	20.638661143284846
37	23.732145875227218	29.533214449553153	26.504575415892024	20.230064259327605
38	23.549129239546218	27.86805969943329	26.158478747512238	22.424332313508256
39	22.440060844580728	28.717745301273805	25.20269454571794	23.639499308427524
40	24.937379061048087	24.808998375408304	26.953597384096938	23.300025179446674
41	20.923775261366107	24.60383762361211	27.641513664161373	26.830873450860416
42	23.081688333638017	25.251328819428874	26.453388705121085	25.213594141812024
43	22.4966283686133	24.359217574563928	28.95408717607332	24.19006688074945
44	23.982905570176495	25.71318195771923	27.930766869366963	22.373145602737317
45	21.25276370296531	26.949251342616385	27.399928945671032	24.39805600874727
46	23.44861840721029	26.070247206978504	27.227811906084114	23.253322479727096
47	22.303401984692275	24.831487415450525	28.481748350401322	24.383362249455885
48	23.08382686198559	23.848454223421026	29.028383789955125	24.03933512463826
49	24.056857260131277	22.791814265363776	28.283279122789466	24.86804935171548
50	21.472204305340455	25.16247641582649	29.209606821215583	24.155712457617472
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	638.0
1	1066.0
2	1494.0
3	9300.5
4	17107.0
5	11495.0
6	5883.0
7	6116.0
8	6349.0
9	6674.5
10	7000.0
11	7155.0
12	7310.0
13	7318.0
14	7326.0
15	7202.5
16	7079.0
17	7116.0
18	7153.0
19	6800.5
20	6448.0
21	6373.0
22	6298.0
23	6884.5
24	7471.0
25	8254.0
26	9037.0
27	10333.0
28	11629.0
29	14014.5
30	16400.0
31	19354.5
32	22309.0
33	25521.0
34	28733.0
35	32860.5
36	36988.0
37	41595.0
38	46202.0
39	56711.5
40	67221.0
41	79463.5
42	91706.0
43	105464.5
44	119223.0
45	129642.0
46	140061.0
47	134159.5
48	128258.0
49	115734.5
50	103211.0
51	94978.0
52	86745.0
53	82198.5
54	77652.0
55	74977.0
56	72302.0
57	69433.5
58	66565.0
59	63052.0
60	59539.0
61	55002.5
62	50466.0
63	44684.5
64	38903.0
65	33439.5
66	27976.0
67	24088.5
68	20201.0
69	17770.5
70	15340.0
71	13125.0
72	10910.0
73	8675.5
74	6441.0
75	5148.0
76	3855.0
77	3110.0
78	2365.0
79	1704.5
80	1044.0
81	727.5
82	411.0
83	324.5
84	238.0
85	157.5
86	77.0
87	47.5
88	18.0
89	13.5
90	9.0
91	6.5
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1449595.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.24162125265705
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.35014588425169	23.649276224271844
2	7.4350578631674145	4.794366392316667
3	3.3729310189017285	3.2624629326830448
4	2.2013009673368327	2.8389404820792694
5	1.6479125573065718	2.6565686265087995
6	1.2658051015343803	2.448696519801155
7	1.0769164030317313	2.4305071551126
8	0.8952831146837579	2.309230327802627
9	0.7855447606196199	2.2794512984016237
>10	7.83440203715352	48.102305078815704
>50	0.10730417412623873	2.2547601825601458
>100	0.0239446407681688	1.4548634583562492
>500	0.002804325158646982	0.6186540213830224
>1k	2.157173198959217E-4	0.09785792108891653
>5k	4.314346397918434E-4	0.8020593788183265
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6020	0.41528840814158435	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5512	0.38024413715555033	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06091356551312608	0.0
2	0.0	0.0	0.0	0.19495100355616568	0.0
3	0.0	0.0	0.0	0.31415671273700585	0.0
4	0.0	0.0	0.0	0.5144195447694011	0.0
5	0.0	0.0	0.0	0.8296800140728963	0.0
6	0.0	0.0	0.0	1.5698867614747567	0.0
7	0.0	0.0	0.0	1.9692396841876525	0.0
8	0.0	0.0	0.0	3.1052811302467243	0.0
9	0.0	0.0	0.0	4.264432479416665	0.0
10	0.0	0.0	0.0	5.754710798533384	0.0
11	0.0	0.0	0.0	6.6646891028183735	0.0
12	0.0	0.0	0.0	7.442216619124652	0.0
13	0.0	0.0	0.0	7.869991273424646	0.0
14	0.0	0.0	0.0	8.088328119233303	0.0
15	0.0	0.0	0.0	8.275966735536477	0.0
16	0.0	0.0	0.0	8.56101186883233	0.0
17	0.0	0.0	0.0	8.860336852707135	0.0
18	0.0	0.0	0.0	9.313152984109355	0.0
19	0.0	0.0	0.0	9.529696225497467	0.0
20	0.0	0.0	0.0	9.783767190146213	0.0
21	0.0	0.0	0.0	10.053704655438244	0.0
22	0.0	0.0	0.0	10.341026286652479	0.0
23	0.0	0.0	0.0	10.642696753231075	0.0
24	0.0	0.0	0.0	10.898699291871178	0.0
25	0.0	0.0	0.0	11.103170195813313	0.0
26	0.0	0.0	0.0	11.311021354240323	0.0
27	0.0	0.0	0.0	11.545017746336045	0.0
28	0.0	0.0	0.0	11.761698957294968	0.0
29	0.0	0.0	0.0	11.99541941024907	0.0
30	0.0	0.0	0.0	12.31123175783581	0.0
31	0.0	0.0	0.0	12.548608404416406	0.0
32	0.0	0.0	0.0	12.790676016404582	0.0
33	0.0	0.0	0.0	13.030467130474374	0.0
34	0.0	0.0	0.0	13.275432103449583	0.0
35	0.0	6.89847854055788E-5	0.0	13.560201297603813	0.0
36	0.0	6.89847854055788E-5	0.0	13.799164594248738	0.0
37	0.0	6.89847854055788E-5	0.0	14.040542358382858	0.0
38	0.0	6.89847854055788E-5	0.0	14.350766938351747	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTACGA	50	2.7284841E-11	44.0	2
TACGAGT	50	2.7284841E-11	44.0	4
TCATACG	30	2.528177E-6	44.0	1
AATCGTA	20	7.857164E-4	44.0	12
CGTTTTT	6380	0.0	41.689655	1
CTATCTC	935	0.0	40.941177	6
ATACGAA	230	0.0	40.173916	19
GTTACGA	55	7.8216544E-11	40.0	2
GCTAACG	55	7.8216544E-11	40.0	1
GAGCGAT	1430	0.0	39.692307	7
AACCGAT	50	1.3478711E-9	39.600002	8
AATACGG	50	1.3478711E-9	39.600002	2
GTTTTTA	3355	0.0	39.34426	2
TACGAAA	235	0.0	39.319153	20
TATCTCT	965	0.0	39.212437	7
AGCGATA	275	0.0	39.2	8
ATTAACG	45	2.3515895E-8	39.11111	1
GCTATCT	590	0.0	38.77966	5
TAGCATA	685	0.0	38.218975	30
ATGTCTC	1255	0.0	38.215137	8
>>END_MODULE