FastQCFastQC Report
Thu 26 May 2016
SRR1546736_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546736_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1726036
Sequences flagged as poor quality0
Sequence length50
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89170.5166172663837834No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82700.4791325325775361No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGCG650.044.0000041
AACGTAC207.857581E-444.039
TATGCGA1050.043.9999962
CGTTTTT96650.042.4066241
CGTTAGA1650.041.3333322
GAGCGAT17950.039.832877
ATATGCG1000.039.6000021
CTATCTC12100.038.7272726
GTTTTTA56600.038.286222
TACGTTG357.2923303E-637.7142871
GTTACGA950.037.052632
TATCTCT12750.036.752947
ACGTAGT301.3013661E-436.66666422
GCGATAT2650.036.52839
AGCGATA4100.036.4878048
TCGTAAG1150.036.3478281
GTAACGA850.036.2352942
CGTAAGA1300.035.5384642
AGCGATG8800.035.08
GCCAGTA9550.034.7853425
Produced by FastQC (version 0.11.5)