FastQCFastQC Report
Thu 26 May 2016
SRR1546735_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546735_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3104982
Sequences flagged as poor quality0
Sequence length50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95290.3068938885958115No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87190.2808067808444622No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA38160.1228992631841344No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATTA1050.041.90476225
CGTTTTT102800.041.3463061
TATGCGA3000.040.3333322
TATAGCG1250.038.721
AGCGATA7200.038.1944478
GAGCGAT31350.038.0350887
ATGTCTC24350.037.6755648
GTTTTTA61150.037.3802152
CGTTCAT1250.036.9617
CTATCTC20700.036.6666686
CGACGGT1200.036.66666428
CGGACTA301.3016058E-436.66666414
CGAATAT3400.036.23529414
TATCTCT21550.035.6287737
TACGTTA250.002353359235.21
GCGATAT4200.035.0952389
AGACCGA12250.035.020418
GACCGAT24000.034.8333329
GTTGATC15300.034.79738616
GTTACGA950.034.7368432
Produced by FastQC (version 0.11.5)