##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546735_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3104982
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.488805088081026	31.0	31.0	33.0	30.0	34.0
2	31.867177651915533	31.0	31.0	34.0	30.0	34.0
3	31.99065340797467	33.0	31.0	34.0	30.0	34.0
4	35.68641814992808	37.0	35.0	37.0	33.0	37.0
5	35.62862200167344	37.0	35.0	37.0	33.0	37.0
6	35.65966501577143	37.0	35.0	37.0	33.0	37.0
7	36.01287543695906	37.0	35.0	37.0	35.0	37.0
8	18.694422383124927	32.0	0.0	37.0	0.0	37.0
9	28.041101365482955	31.0	17.0	39.0	17.0	39.0
10	33.59338411623642	32.0	32.0	39.0	27.0	39.0
11	35.74649031781827	37.0	35.0	39.0	32.0	39.0
12	36.490619913416566	37.0	35.0	39.0	32.0	39.0
13	36.71668370380247	39.0	35.0	39.0	33.0	39.0
14	37.84111019001077	40.0	36.0	41.0	33.0	41.0
15	38.040875277215775	40.0	37.0	41.0	33.0	41.0
16	38.11011625832291	40.0	37.0	41.0	33.0	41.0
17	38.04989851793022	40.0	37.0	41.0	33.0	41.0
18	38.03243497063751	40.0	36.0	41.0	33.0	41.0
19	38.040397657699785	40.0	37.0	41.0	33.0	41.0
20	37.976831105623155	40.0	36.0	41.0	33.0	41.0
21	37.8282659931684	40.0	36.0	41.0	33.0	41.0
22	37.80849550818652	40.0	36.0	41.0	33.0	41.0
23	37.675195862649126	39.0	36.0	41.0	33.0	41.0
24	37.56799041025037	39.0	35.0	41.0	33.0	41.0
25	37.41349482863347	39.0	35.0	41.0	32.0	41.0
26	37.40100425702951	39.0	35.0	41.0	33.0	41.0
27	37.270849235196856	39.0	35.0	41.0	32.0	41.0
28	37.26400958202012	39.0	35.0	41.0	32.0	41.0
29	37.16250110306598	39.0	35.0	41.0	32.0	41.0
30	37.0714397056086	39.0	35.0	41.0	32.0	41.0
31	37.10494070497027	39.0	35.0	41.0	32.0	41.0
32	36.963479015337285	39.0	35.0	41.0	31.0	41.0
33	36.904585598241795	39.0	35.0	41.0	31.0	41.0
34	36.78002932062086	39.0	35.0	41.0	31.0	41.0
35	36.630662271150044	39.0	35.0	41.0	31.0	41.0
36	36.50442901118267	39.0	35.0	41.0	30.0	41.0
37	36.44770340053501	39.0	35.0	41.0	30.0	41.0
38	36.34560876681411	39.0	35.0	41.0	30.0	41.0
39	36.259477832721736	39.0	35.0	41.0	30.0	41.0
40	36.141688744089336	39.0	35.0	40.0	30.0	41.0
41	36.08959472228825	39.0	35.0	40.0	30.0	41.0
42	35.92764563530481	38.0	35.0	40.0	29.0	41.0
43	35.91174473797272	38.0	35.0	40.0	29.0	41.0
44	35.64953774289191	38.0	35.0	40.0	28.0	41.0
45	35.53639248150231	38.0	35.0	40.0	28.0	41.0
46	35.510753363465554	38.0	35.0	40.0	28.0	41.0
47	35.372942258602464	38.0	34.0	40.0	28.0	41.0
48	35.33798875484624	38.0	34.0	40.0	28.0	41.0
49	35.296722493077255	38.0	34.0	40.0	28.0	41.0
50	34.67958397182335	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	3.0
12	2.0
13	10.0
14	25.0
15	68.0
16	159.0
17	364.0
18	845.0
19	1794.0
20	3185.0
21	5376.0
22	8708.0
23	13017.0
24	18475.0
25	26115.0
26	34554.0
27	41035.0
28	46918.0
29	55413.0
30	69721.0
31	89515.0
32	117611.0
33	157125.0
34	234630.0
35	341475.0
36	349047.0
37	503438.0
38	532967.0
39	453384.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.020416865540604	25.0893886019307	28.05562157848258	13.83457295404611
2	31.308233026793715	26.106979042068524	28.90332375517797	13.681464175959796
3	29.25466234586867	25.84607575824916	31.020212033435296	13.879049862446868
4	25.7748998222856	28.598587689075174	29.73440747804657	15.892105010592655
5	23.359426882345854	31.859218507546906	29.035949322733597	15.745405287373645
6	21.866535780239627	40.02110157160331	26.692972777297904	11.419389870859154
7	85.66452237082211	4.326723955243541	7.66890114016764	2.339852533766701
8	44.20431422790857	49.99304343793297	3.7203758347069322	2.0822664994515265
9	79.3845503774257	5.357164711421838	10.534972505476683	4.723312405675782
10	42.9425999893075	25.042625045813473	16.279450251241393	15.73532471363763
11	34.2484111019001	24.98658607360687	24.319464653901377	16.44553817059165
12	30.371641445908544	21.616936909779184	27.687471296129896	20.323950348182372
13	23.912505772980328	26.944439613498567	30.133218163583557	19.009836449937552
14	19.903593644021125	29.506644482963186	30.876249846214886	19.7135120268008
15	19.16275199018867	23.303323497527522	38.438097225684395	19.095827286599405
16	21.960674812285546	20.781795192371487	36.39135428160292	20.86617571374005
17	21.679288317935498	22.65768368383456	29.400299260994107	26.262728737235836
18	24.53750134461327	22.509566883157454	30.983045956466093	21.96988581576318
19	27.433750018518626	26.025819151286544	25.816864638828825	20.723566191366004
20	29.541684943745246	24.222233816492334	24.88339706961264	21.35268417014978
21	25.37064627105729	27.43478062030633	26.70170068618755	20.492872422448826
22	24.354279670542372	24.18919014667396	26.502375859183726	24.954154323599944
23	23.278170372646283	27.93017801713504	25.42552581625272	23.366125793965956
24	23.22416039770923	24.205422124830353	30.935348417478746	21.635069059981667
25	22.695719331062143	25.248036864625945	28.605415425918736	23.450828378393176
26	21.09139441066003	27.84161067600392	27.876586724174246	23.190408189161804
27	22.091303588877487	26.01068218752959	28.018519914125108	23.879494309467816
28	21.183826508495056	26.59219924624362	30.523655209595418	21.700319035665906
29	22.28334979075563	24.226130779502103	29.92993196095823	23.56058746878404
30	23.768157110089525	24.33801548608011	29.92993196095823	21.963895442872133
31	25.953032900029694	24.52410352137307	26.202084263290416	23.32077931530682
32	27.14176764953871	24.076693520284497	26.497931388974234	22.28360744120256
33	25.33177325987719	23.88352009770105	27.367276203211482	23.41743043921028
34	21.379254372489115	24.641946394536266	29.654890108863757	24.323909124110866
35	21.69220304658771	24.277660868887484	30.904462570153385	23.12567351437142
36	25.59390038331945	24.898083145087476	26.1760615681508	23.331954903442277
37	22.90306352822657	26.018604938772594	28.398715354871623	22.679616178129216
38	23.95418073277075	26.64682114099212	25.781663146517435	23.61733497971969
39	23.031148006655112	25.325879505903735	26.05264700407281	25.590325483368343
40	25.41625040016335	23.065286690872927	27.773655370626948	23.744807538336776
41	21.017094463027483	23.06812084578912	28.1417412403679	27.773043450815493
42	23.9898009070584	24.817567380422815	26.270361631726047	24.92227008079274
43	22.922580549581287	23.50879972895173	28.3434493340058	25.225170387461183
44	23.288186533770567	25.59229006802616	27.292460954685083	23.82706244351819
45	21.82392683757909	25.760729047704622	27.222315620509235	25.193028494207052
46	23.331568427771884	25.68311185056789	27.25152673992957	23.73379298173065
47	23.23404773361005	24.32403794933433	28.306057812895535	24.13585650416009
48	23.4297654543569	22.71942317217942	29.05053877929083	24.80027259417285
49	23.39449954943378	22.717973888415457	28.37646079751831	25.511065764632452
50	21.728370728075074	24.822398326302697	28.984709090101006	24.464521855521223
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1404.0
1	2404.0
2	3404.0
3	16244.0
4	29084.0
5	19916.0
6	10748.0
7	10912.5
8	11077.0
9	11815.5
10	12554.0
11	12680.0
12	12806.0
13	12701.0
14	12596.0
15	12504.0
16	12412.0
17	12470.0
18	12528.0
19	12000.5
20	11473.0
21	12177.0
22	12881.0
23	14352.5
24	15824.0
25	18015.0
26	20206.0
27	23981.0
28	27756.0
29	33053.5
30	38351.0
31	43590.0
32	48829.0
33	55208.5
34	61588.0
35	69973.0
36	78358.0
37	86221.5
38	94085.0
39	111992.5
40	129900.0
41	153153.0
42	176406.0
43	193309.5
44	210213.0
45	233284.5
46	256356.0
47	270837.0
48	285318.0
49	276489.0
50	267660.0
51	243266.0
52	218872.0
53	201547.0
54	184222.0
55	172354.0
56	160486.0
57	156287.0
58	152088.0
59	145286.5
60	138485.0
61	128505.0
62	118525.0
63	104778.5
64	91032.0
65	77338.5
66	63645.0
67	53512.5
68	43380.0
69	36839.5
70	30299.0
71	25785.0
72	21271.0
73	17111.0
74	12951.0
75	10419.0
76	7887.0
77	6190.5
78	4494.0
79	3316.5
80	2139.0
81	1433.5
82	728.0
83	556.0
84	384.0
85	304.0
86	224.0
87	129.0
88	34.0
89	20.5
90	7.0
91	8.5
92	10.0
93	6.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3104982.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.766367288016767
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.49973595355058	25.2541781160084
2	7.218815769436084	4.586311062328679
3	2.553342101027783	2.433312089796149
4	1.5060953702405269	1.913727148073687
5	0.966160760542481	1.5345708789331038
6	0.7351249335912122	1.401134918582243
7	0.5969797841869864	1.3274715361603358
8	0.49882627576363203	1.2676718951056856
9	0.40814193035251034	1.1668667818696203
>10	5.1948957182801445	38.50232113635981
>50	0.7570160221006393	15.397087005874557
>100	0.06011040744109433	2.9150506552210462
>500	0.0025095693400252633	0.5515358440902088
>1k	0.001981238952651524	0.9831142155099037
>5k	2.6416519368686984E-4	0.7656467160865829
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9529	0.3068938885958115	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8719	0.2808067808444622	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3816	0.1228992631841344	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04734326962281907	0.0
2	0.0	0.0	0.0	0.18199783444799358	0.0
3	0.0	0.0	0.0	0.304188558903079	0.0
4	0.0	0.0	0.0	0.49346501847675767	0.0
5	0.0	0.0	0.0	0.8086681339859619	0.0
6	0.0	0.0	0.0	1.497593222762644	0.0
7	0.0	0.0	0.0	1.8387868271056	0.0
8	0.0	0.0	0.0	2.7792753710005402	0.0
9	0.0	0.0	0.0	3.6875898153354836	0.0
10	0.0	0.0	0.0	4.919481014704755	0.0
11	0.0	0.0	0.0	5.728922100031498	0.0
12	0.0	0.0	0.0	6.431695900330501	0.0
13	0.0	0.0	0.0	6.803066813269771	0.0
14	0.0	0.0	0.0	6.979653988332299	0.0
15	0.0	0.0	0.0	7.149026950880875	0.0
16	0.0	0.0	0.0	7.421395679588481	0.0
17	0.0	0.0	0.0	7.707967389182932	0.0
18	0.0	0.0	0.0	8.13618243197545	0.0
19	0.0	0.0	0.0	8.330450868958339	0.0
20	0.0	0.0	0.0	8.564719537826628	0.0
21	0.0	0.0	0.0	8.800115427400224	0.0
22	0.0	0.0	0.0	9.042210228593918	0.0
23	0.0	0.0	0.0	9.313226292455157	0.0
24	0.0	0.0	0.0	9.531552839919845	0.0
25	0.0	0.0	0.0	9.721731076057768	0.0
26	3.2206305865863314E-5	0.0	0.0	9.898318251120296	0.0
27	3.2206305865863314E-5	0.0	0.0	10.089784739492854	0.0
28	3.2206305865863314E-5	0.0	0.0	10.274198046880787	0.0
29	3.2206305865863314E-5	0.0	0.0	10.474392444142994	0.0
30	3.2206305865863314E-5	0.0	0.0	10.727888277613204	0.0
31	3.2206305865863314E-5	0.0	0.0	10.950079581781795	0.0
32	3.2206305865863314E-5	0.0	0.0	11.186699310978293	0.0
33	3.2206305865863314E-5	0.0	0.0	11.386636057793572	0.0
34	3.2206305865863314E-5	0.0	0.0	11.589278134301583	0.0
35	3.2206305865863314E-5	0.0	0.0	11.808892934000905	0.0
36	3.2206305865863314E-5	0.0	0.0	12.01205031140277	0.0
37	6.441261173172663E-5	0.0	0.0	12.23060230300852	0.0
38	6.441261173172663E-5	0.0	0.0	12.473083579872604	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATTA	105	0.0	41.904762	25
CGTTTTT	10280	0.0	41.346306	1
TATGCGA	300	0.0	40.333332	2
TATAGCG	125	0.0	38.72	1
AGCGATA	720	0.0	38.194447	8
GAGCGAT	3135	0.0	38.035088	7
ATGTCTC	2435	0.0	37.675564	8
GTTTTTA	6115	0.0	37.380215	2
CGTTCAT	125	0.0	36.96	17
CTATCTC	2070	0.0	36.666668	6
CGACGGT	120	0.0	36.666664	28
CGGACTA	30	1.3016058E-4	36.666664	14
CGAATAT	340	0.0	36.235294	14
TATCTCT	2155	0.0	35.628773	7
TACGTTA	25	0.0023533592	35.2	1
GCGATAT	420	0.0	35.095238	9
AGACCGA	1225	0.0	35.02041	8
GACCGAT	2400	0.0	34.833332	9
GTTGATC	1530	0.0	34.797386	16
GTTACGA	95	0.0	34.736843	2
>>END_MODULE