FastQCFastQC Report
Thu 26 May 2016
SRR1546734_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546734_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1764718
Sequences flagged as poor quality0
Sequence length50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52290.2963079653519713No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51880.29398464797208396No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA22200.1257991361792649No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGTA207.857628E-444.0000041
GTAATCG254.443812E-544.01
TATTACG254.443812E-544.01
GAGCGAT17700.040.644077
CGTTTTT55850.040.5729641
TATGCGA1100.040.02
TATCTCT11500.039.4086957
AGCGATA3700.039.2432448
GCGTAAG1350.039.1111151
ACGTGCG452.3521352E-839.111111
CTATCTC11600.039.0689626
TACGAGC1400.037.7142874
TATCGCG357.292425E-637.7142871
GTTTTTA33300.036.79882
AGCGCGA3650.036.7671248
CGTTGTA301.3013782E-436.66666432
TCGTAAG601.9826984E-1036.6666641
ACGAATC301.3013782E-436.66666439
CGTAAGA1150.036.3478282
ATGTCTC16300.036.171788
Produced by FastQC (version 0.11.5)