##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546734_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1764718
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.45237709367729	31.0	31.0	33.0	30.0	34.0
2	31.822828349911997	31.0	31.0	34.0	30.0	34.0
3	31.944651213395	31.0	31.0	34.0	30.0	34.0
4	35.639935672441716	37.0	35.0	37.0	33.0	37.0
5	35.58142547421174	37.0	35.0	37.0	33.0	37.0
6	35.614575813246084	37.0	35.0	37.0	33.0	37.0
7	35.98114996277025	37.0	35.0	37.0	35.0	37.0
8	18.43902028539404	30.0	0.0	37.0	0.0	37.0
9	27.87624594977781	29.0	17.0	39.0	17.0	39.0
10	33.50982536586582	32.0	32.0	39.0	27.0	39.0
11	35.68415180215762	37.0	34.0	39.0	32.0	39.0
12	36.418011262989324	37.0	35.0	39.0	32.0	39.0
13	36.62906934705715	39.0	35.0	39.0	33.0	39.0
14	37.717606439102454	39.0	36.0	41.0	33.0	41.0
15	37.94227972967919	40.0	36.0	41.0	33.0	41.0
16	38.02387010275863	40.0	37.0	41.0	33.0	41.0
17	37.970576602040666	40.0	36.0	41.0	33.0	41.0
18	37.95499620902603	40.0	36.0	41.0	33.0	41.0
19	37.96411664639903	40.0	36.0	41.0	33.0	41.0
20	37.8957725823616	40.0	36.0	41.0	33.0	41.0
21	37.740418015796294	40.0	36.0	41.0	33.0	41.0
22	37.742077204403195	40.0	36.0	41.0	33.0	41.0
23	37.60768576055778	39.0	36.0	41.0	33.0	41.0
24	37.476501628022156	39.0	35.0	41.0	32.0	41.0
25	37.31814601539736	39.0	35.0	41.0	32.0	41.0
26	37.29023844036271	39.0	35.0	41.0	32.0	41.0
27	37.158540344689634	39.0	35.0	41.0	32.0	41.0
28	37.15459977174823	39.0	35.0	41.0	32.0	41.0
29	37.05620558072168	39.0	35.0	41.0	32.0	41.0
30	36.92476531661149	39.0	35.0	40.0	31.0	41.0
31	36.9357398745862	39.0	35.0	41.0	31.0	41.0
32	36.74912422268034	39.0	35.0	41.0	31.0	41.0
33	36.648578413094896	39.0	35.0	41.0	31.0	41.0
34	36.53111885298387	39.0	35.0	41.0	30.0	41.0
35	36.36803613948518	39.0	35.0	41.0	30.0	41.0
36	36.23436379070197	39.0	35.0	41.0	30.0	41.0
37	36.17434626948895	39.0	35.0	41.0	30.0	41.0
38	36.07477625320306	39.0	35.0	40.0	29.0	41.0
39	35.99104332816915	39.0	35.0	40.0	29.0	41.0
40	35.89458769049786	38.0	35.0	40.0	29.0	41.0
41	35.86859090234247	38.0	35.0	40.0	29.0	41.0
42	35.694832828814576	38.0	35.0	40.0	28.0	41.0
43	35.67147838918173	38.0	35.0	40.0	28.0	41.0
44	35.4003234511123	38.0	34.0	40.0	27.0	41.0
45	35.28381361781316	38.0	34.0	40.0	27.0	41.0
46	35.269532582542936	38.0	34.0	40.0	27.0	41.0
47	35.10440874972659	37.0	34.0	40.0	27.0	41.0
48	35.039965025573494	37.0	34.0	40.0	27.0	41.0
49	34.961202866406985	37.0	34.0	40.0	27.0	41.0
50	34.31500160365565	36.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	2.0
11	4.0
12	0.0
13	3.0
14	8.0
15	30.0
16	93.0
17	230.0
18	513.0
19	1070.0
20	1920.0
21	3419.0
22	5387.0
23	7974.0
24	11404.0
25	16087.0
26	21112.0
27	25062.0
28	29132.0
29	34781.0
30	42819.0
31	54950.0
32	70074.0
33	92157.0
34	136464.0
35	195407.0
36	199465.0
37	287050.0
38	292337.0
39	235763.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.873287403426495	24.930555476852394	27.930694875895185	13.265462243825926
2	31.28233519463166	26.159873702200574	28.230232819067975	14.327558284099782
3	29.99442403828827	25.249813284615445	30.74485555199188	14.010907125104408
4	25.88617558159434	28.562070540448957	29.622806590061412	15.92894728789529
5	23.915435780674308	31.133302884653524	29.501937420029716	15.449323914642452
6	22.02856207054045	39.46715565886448	26.768752854563733	11.735529416031342
7	84.85168735174685	4.441786166401657	8.06310130003774	2.6434251818137513
8	43.26526957848223	50.630979000610864	3.8622034795361073	2.2415479413708024
9	78.13135016472887	5.682210982151256	11.292285792970889	4.894153060148987
10	43.67944340115531	24.916388907462835	16.357117681125256	15.047050010256596
11	34.81950090609377	24.40242577000971	24.302523122674557	16.475550201221953
12	30.39692460778436	21.927752762764364	27.82070563115467	19.854616998296613
13	24.22126368065606	27.4538481502427	29.978727479404643	18.346160689696596
14	19.575762246432575	29.998334011439788	31.00393377298809	19.421969969139546
15	19.135011939584682	23.038978465681204	38.7165541463282	19.10945544840592
16	22.06499848701039	21.548882031010052	35.778464321211665	20.607655160767894
17	21.68493776342736	23.026738549728627	29.115756738470395	26.17256694837362
18	24.12663099713382	22.27024374432629	31.8253114661946	21.777813792345295
19	27.289232613936047	25.713400101319305	26.25382639039212	20.743540894352524
20	29.05994045507554	24.17587399233192	25.37946572766867	21.38471982492387
21	25.191900348950934	27.70731640976065	27.243106264003654	19.857676977284754
22	24.578204562995335	23.41467588589225	26.78189943095724	25.22522012015517
23	22.68317090889309	27.906781706765614	25.604997512350415	23.80504987199088
24	22.667191018621672	24.24070021385853	31.743258696290283	21.34885007122951
25	22.918392627037296	24.738740127317794	28.99318758011195	23.349679665532964
26	21.096571803540282	27.371115385007688	28.036434149818838	23.495878661633192
27	22.93397585336581	25.87744897485037	27.5619674078238	23.62660776396002
28	21.636488096115073	26.538517768844656	30.164196205852722	21.660797929187552
29	23.019428599923614	24.75353002576049	29.014097436530932	23.21294393778496
30	25.562611136736862	25.148323981508657	28.006287690157862	21.28277719159662
31	28.668829807368656	24.64898074366556	24.343832839014507	22.33835660995128
32	30.026837149051577	25.111377568540693	24.532474877005843	20.32931040540188
33	28.37722514305402	25.677020351126924	24.128387651738127	21.817366854080937
34	24.615434307351087	26.773002825380598	27.422568365030557	21.18899450223775
35	23.792299959540276	27.103423889822626	28.102393696896616	21.001882453740485
36	27.31739575388249	28.52030749388854	24.341226190246825	19.82107056198214
37	23.699707262010133	28.87452839490502	27.631213598999953	19.79455074408489
38	23.562234872653875	30.57882335874627	25.013004910699614	20.845936857900245
39	22.749810451301567	27.294162580083615	27.082230702015845	22.87379626659897
40	24.646714092563233	24.7959730676516	27.94327478951311	22.614038050272057
41	21.09946178369575	24.07183470673501	28.561560543950932	26.2671429656183
42	24.329779602180064	25.30931287605158	26.45261169206638	23.90829582970197
43	23.885119322180653	23.463352218314768	28.642763319691873	24.008765139812706
44	23.913225795849534	25.555357853209408	27.640223537131714	22.891192813809344
45	22.07134511009691	26.967481489960438	27.013947837558185	23.94722556238447
46	24.164767401930508	26.394472091291636	26.650716998409944	22.790043508367912
47	23.157750983443247	25.12718745997944	28.220032889107493	23.495028667469818
48	23.572548135169473	24.165787394926554	29.01302077725733	23.248643692646645
49	24.083337961079334	22.964972307190155	28.7204527862242	24.231236945506307
50	22.032472043691968	25.335096032340576	28.73733933693655	23.895092587030902
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	828.0
1	1357.0
2	1886.0
3	9133.0
4	16380.0
5	11260.5
6	6141.0
7	6312.0
8	6483.0
9	6846.0
10	7209.0
11	7408.0
12	7607.0
13	7668.5
14	7730.0
15	7524.5
16	7319.0
17	7246.0
18	7173.0
19	7179.5
20	7186.0
21	7389.0
22	7592.0
23	8282.0
24	8972.0
25	10810.5
26	12649.0
27	14724.5
28	16800.0
29	18787.0
30	20774.0
31	23458.5
32	26143.0
33	29801.5
34	33460.0
35	37919.0
36	42378.0
37	48799.0
38	55220.0
39	67506.5
40	79793.0
41	95584.5
42	111376.0
43	127750.5
44	144125.0
45	155777.5
46	167430.0
47	161058.0
48	154686.0
49	140465.5
50	126245.0
51	117692.5
52	109140.0
53	103927.5
54	98715.0
55	94374.5
56	90034.0
57	86837.0
58	83640.0
59	79352.5
60	75065.0
61	70566.5
62	66068.0
63	57908.0
64	49748.0
65	42538.5
66	35329.0
67	30387.0
68	25445.0
69	21753.5
70	18062.0
71	15357.5
72	12653.0
73	10211.0
74	7769.0
75	6159.5
76	4550.0
77	3587.5
78	2625.0
79	2018.0
80	1411.0
81	957.0
82	503.0
83	364.0
84	225.0
85	146.0
86	67.0
87	48.5
88	30.0
89	28.0
90	26.0
91	20.0
92	14.0
93	10.0
94	6.0
95	6.0
96	6.0
97	3.0
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1764718.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.489696749659643
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.75300912488335	22.0444357021735
2	6.914360906427971	4.078048126965253
3	2.856246446876442	2.5268952468203736
4	1.827881345427297	2.156146662840434
5	1.362824357821712	2.009463851760597
6	1.0882401100420906	1.9255122501574498
7	0.9080194549505163	1.8744052858497375
8	0.8067967590558407	1.9033753410531966
9	0.6952776737372293	1.8453174979787719
>10	8.54330538623082	52.539255414092636
>50	0.2079012854163172	3.925385982538845
>100	0.03181642504260626	1.5779476809543624
>500	0.0033387413406196324	0.7294614266900316
>1k	5.89189648344641E-4	0.26103077809384456
>5k	3.9279309889642735E-4	0.6033187520309693
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5229	0.2963079653519713	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5188	0.29398464797208396	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2220	0.1257991361792649	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.6666277558227435E-5	0.0	0.0	0.07513948404220958	0.0
2	5.6666277558227435E-5	0.0	0.0	0.23442839025838688	0.0
3	5.6666277558227435E-5	0.0	0.0	0.3639108344789366	0.0
4	5.6666277558227435E-5	0.0	0.0	0.5896692842709147	0.0
5	5.6666277558227435E-5	0.0	0.0	0.9404902086339008	0.0
6	5.6666277558227435E-5	0.0	0.0	1.6143655813563413	0.0
7	5.6666277558227435E-5	0.0	0.0	1.946656632957787	0.0
8	5.6666277558227435E-5	0.0	0.0	2.8973467715521686	0.0
9	5.6666277558227435E-5	0.0	0.0	3.8040638787613656	0.0
10	5.6666277558227435E-5	0.0	0.0	5.0412587166901455	0.0
11	5.6666277558227435E-5	0.0	0.0	5.858556437912459	0.0
12	5.6666277558227435E-5	0.0	0.0	6.5469383776898065	0.0
13	5.6666277558227435E-5	0.0	0.0	6.898439297383491	0.0
14	1.1333255511645487E-4	0.0	0.0	7.070251450940037	0.0
15	1.1333255511645487E-4	0.0	0.0	7.245803578815425	0.0
16	1.1333255511645487E-4	0.0	0.0	7.555031455450672	0.0
17	1.1333255511645487E-4	0.0	0.0	7.869812627286626	0.0
18	1.1333255511645487E-4	0.0	0.0	8.34150272168131	0.0
19	1.1333255511645487E-4	0.0	0.0	8.554964589243154	0.0
20	1.1333255511645487E-4	0.0	0.0	8.800442903625395	0.0
21	1.1333255511645487E-4	0.0	0.0	9.05476115730672	0.0
22	1.1333255511645487E-4	0.0	0.0	9.31242272136398	0.0
23	1.1333255511645487E-4	0.0	0.0	9.599097419531052	0.0
24	1.1333255511645487E-4	0.0	0.0	9.828482511086758	0.0
25	1.1333255511645487E-4	0.0	0.0	10.023244507054384	0.0
26	1.1333255511645487E-4	0.0	0.0	10.215399854254334	0.0
27	1.1333255511645487E-4	0.0	0.0	10.417755131414765	0.0
28	1.1333255511645487E-4	0.0	0.0	10.61387711804379	0.0
29	1.1333255511645487E-4	0.0	0.0	10.829775635540637	0.0
30	1.1333255511645487E-4	0.0	0.0	11.089817183255342	0.0
31	1.1333255511645487E-4	0.0	0.0	11.309059011128124	0.0
32	1.1333255511645487E-4	0.0	0.0	11.537650774798013	0.0
33	1.1333255511645487E-4	0.0	0.0	11.753379293462185	0.0
34	1.1333255511645487E-4	0.0	0.0	11.977097757262067	0.0
35	1.1333255511645487E-4	0.0	0.0	12.216966110166043	0.0
36	1.1333255511645487E-4	0.0	0.0	12.432807961385333	0.0
37	1.1333255511645487E-4	0.0	0.0	12.658113080956845	0.0
38	1.1333255511645487E-4	0.0	0.0	12.911184676531887	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACGTA	20	7.857628E-4	44.000004	1
GTAATCG	25	4.443812E-5	44.0	1
TATTACG	25	4.443812E-5	44.0	1
GAGCGAT	1770	0.0	40.64407	7
CGTTTTT	5585	0.0	40.572964	1
TATGCGA	110	0.0	40.0	2
TATCTCT	1150	0.0	39.408695	7
AGCGATA	370	0.0	39.243244	8
GCGTAAG	135	0.0	39.111115	1
ACGTGCG	45	2.3521352E-8	39.11111	1
CTATCTC	1160	0.0	39.068962	6
TACGAGC	140	0.0	37.714287	4
TATCGCG	35	7.292425E-6	37.714287	1
GTTTTTA	3330	0.0	36.7988	2
AGCGCGA	365	0.0	36.767124	8
CGTTGTA	30	1.3013782E-4	36.666664	32
TCGTAAG	60	1.9826984E-10	36.666664	1
ACGAATC	30	1.3013782E-4	36.666664	39
CGTAAGA	115	0.0	36.347828	2
ATGTCTC	1630	0.0	36.17178	8
>>END_MODULE