FastQCFastQC Report
Thu 26 May 2016
SRR1546733_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546733_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1899281
Sequences flagged as poor quality0
Sequence length50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48940.25767645756473107No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45300.23851131033269957No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA28040.14763481549070412No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACA207.85778E-444.00000422
TCTAGCG650.044.0000041
GAGCGAT20500.040.8878067
CGTTTTT56950.040.7550471
AGCGATA3800.040.5263188
CTATCTC10550.040.0379146
AGACCGA4700.038.8510638
TCACGAC1250.038.7225
GTTTTTA34100.037.7419362
GCGATAA1750.037.7142879
GATCGAC357.2927196E-637.7142839
TATCTCT11700.036.85477
TATAGCG900.036.6666681
GACGATA900.036.6666687
TTATACG301.3014152E-436.6666641
TGATTCG2400.036.66666415
TAACGAG2350.036.5106353
ATTAGCG850.036.235291
GAGACCG6300.035.9682547
GTAGCAT10550.035.65876829
Produced by FastQC (version 0.11.5)