##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546733_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1899281
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.44694860844709	31.0	31.0	33.0	30.0	34.0
2	31.818830388973513	31.0	31.0	34.0	30.0	34.0
3	31.94696677321576	31.0	31.0	34.0	30.0	34.0
4	35.63588431622282	37.0	35.0	37.0	33.0	37.0
5	35.58369509303784	37.0	35.0	37.0	33.0	37.0
6	35.61401446126192	37.0	35.0	37.0	33.0	37.0
7	35.98336896962587	37.0	35.0	37.0	35.0	37.0
8	18.581810169216666	31.0	0.0	37.0	0.0	37.0
9	27.957790342766554	30.0	17.0	39.0	17.0	39.0
10	33.520872898744315	32.0	32.0	39.0	27.0	39.0
11	35.69012115637444	37.0	34.0	39.0	32.0	39.0
12	36.37286583712468	37.0	35.0	39.0	32.0	39.0
13	36.57548303805493	39.0	35.0	39.0	33.0	39.0
14	37.67652811774561	39.0	36.0	41.0	33.0	41.0
15	37.90697900942514	40.0	36.0	41.0	33.0	41.0
16	37.97794849735242	40.0	36.0	41.0	33.0	41.0
17	37.93145353425849	40.0	36.0	41.0	33.0	41.0
18	37.905087767423566	40.0	36.0	41.0	33.0	41.0
19	37.919832294431416	40.0	36.0	41.0	33.0	41.0
20	37.84594749276174	40.0	36.0	41.0	33.0	41.0
21	37.700279210922446	40.0	36.0	41.0	33.0	41.0
22	37.68803826290054	40.0	36.0	41.0	33.0	41.0
23	37.55558129629054	39.0	35.0	41.0	33.0	41.0
24	37.44435025675506	39.0	35.0	41.0	32.0	41.0
25	37.29347000259572	39.0	35.0	41.0	32.0	41.0
26	37.264562747692416	39.0	35.0	41.0	32.0	41.0
27	37.13158611074401	39.0	35.0	41.0	32.0	41.0
28	37.14666023616305	39.0	35.0	41.0	32.0	41.0
29	37.032994591111056	39.0	35.0	41.0	32.0	41.0
30	36.92264651728733	39.0	35.0	40.0	31.0	41.0
31	36.928230735736314	39.0	35.0	41.0	31.0	41.0
32	36.78381661270765	39.0	35.0	41.0	31.0	41.0
33	36.70646734211525	39.0	35.0	41.0	31.0	41.0
34	36.611419795175124	39.0	35.0	41.0	31.0	41.0
35	36.457506814420825	39.0	35.0	41.0	30.0	41.0
36	36.32270264378994	39.0	35.0	41.0	30.0	41.0
37	36.2364921251779	39.0	35.0	41.0	30.0	41.0
38	36.12362151782701	39.0	35.0	40.0	30.0	41.0
39	36.023458877333056	38.0	35.0	40.0	29.0	41.0
40	35.917331348020646	38.0	35.0	40.0	29.0	41.0
41	35.86573287470364	38.0	35.0	40.0	29.0	41.0
42	35.70459874026013	38.0	35.0	40.0	28.0	41.0
43	35.666057839782525	38.0	35.0	40.0	28.0	41.0
44	35.39804010043801	38.0	34.0	40.0	27.0	41.0
45	35.27643724125077	38.0	34.0	40.0	27.0	41.0
46	35.2673127357142	38.0	34.0	40.0	27.0	41.0
47	35.10144312505627	37.0	34.0	40.0	27.0	41.0
48	35.061650171828184	37.0	34.0	40.0	27.0	41.0
49	35.03126025058957	37.0	34.0	40.0	27.0	41.0
50	34.37850639268228	36.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	3.0
13	9.0
14	22.0
15	31.0
16	103.0
17	235.0
18	573.0
19	1138.0
20	2250.0
21	3752.0
22	5859.0
23	8682.0
24	12101.0
25	16879.0
26	21819.0
27	26504.0
28	30810.0
29	36887.0
30	45971.0
31	58538.0
32	75637.0
33	99592.0
34	148748.0
35	217520.0
36	211540.0
37	305613.0
38	314934.0
39	253527.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.32814838878502	25.4710071864037	27.28711549265222	12.913728932159064
2	31.42936721843687	26.220817246105238	28.14891530005302	14.200900235404873
3	29.491633939369688	26.104773332645355	30.171417499569575	14.232175228415384
4	25.292781847446484	28.886826119989617	29.98313572346588	15.837256309098022
5	23.836230657812088	31.38745662174265	29.227902558915712	15.548410161529546
6	21.765868241718838	40.16172435779645	26.581058832263366	11.491348568221342
7	85.83790392258965	4.4132490137057125	7.384426001207825	2.364421062496808
8	43.96953373408148	50.27539368845368	3.5781435185209562	2.1769290589438843
9	79.03738309391817	5.639923739562498	10.730534344312401	4.59215882220693
10	45.46257241556147	23.874508300772764	15.86531956040207	14.7975997232637
11	37.07013338205352	23.78810718371847	23.069098253496982	16.072661180731025
12	31.88553984376193	21.879700792036566	26.645767529923166	19.588991834278342
13	23.94511396681165	28.576656113550335	29.269023383059167	18.209206536578844
14	20.020260298502432	30.52381401172338	29.914478163052227	19.541447526721957
15	18.52674775349198	23.082103174833	39.66327257525348	18.72787649642154
16	21.5161421611652	21.139157396930734	36.97199097974444	20.372709462159627
17	21.71779741912861	22.60365896357622	28.463508032776613	27.21503558451856
18	24.25617904880847	22.276482521543677	31.643079670675377	21.824258758972476
19	27.64361882207004	25.6633957797714	25.63254199878796	21.060443399370605
20	29.124916218295237	24.69513463252673	24.514697930427356	21.665251218750676
21	25.586629887836505	27.86338619719778	26.55378535351009	19.996198561455625
22	24.423505526565055	23.78384241194431	26.29431874483028	25.49833331666036
23	22.890714960029612	27.832637719221115	25.25276670487411	24.023880615875164
24	22.88150094693729	24.35126766392124	31.798559560170403	20.96867182897107
25	22.796837329494686	24.51274982480212	29.282765425442577	23.407647420260613
26	20.851890794463802	28.027342978737746	27.244099214386917	23.87666701241154
27	21.848636405039592	26.976840183206168	27.24678444105954	23.9277389706947
28	20.869634351104445	26.80861862989205	30.77390865280072	21.54783836620279
29	22.13895679470284	24.89600011793937	29.304773753857383	23.660269333500413
30	25.060325460003025	26.141418779001107	27.025542823837018	21.77271293715885
31	27.43359197506846	25.808398020092866	23.977757898910166	22.780252105928504
32	28.98022988699408	25.093548558638766	23.87608784587431	22.050133708492844
33	27.022804945661015	26.8855424763371	23.430182263709266	22.66147031429262
34	22.077881050776583	26.853003847245354	26.45064105837946	24.618474043598603
35	21.675676216420843	28.537746652549046	27.013380326555154	22.773196804474956
36	25.99020366128024	28.19093119975401	23.463510665351784	22.35535447361396
37	22.49609194216127	32.07561177098071	24.560873298895743	20.867422987962286
38	22.66873622175971	31.941139831336173	22.508043833429596	22.88208011347452
39	22.385471133550013	30.17362886271173	23.855079896023813	23.585820107714444
40	24.406657045481946	25.753271896049085	25.97809381550176	23.861977242967207
41	20.26377350165668	25.883531715422837	26.673514872206905	27.17917991071358
42	24.024986297446244	26.57237133420489	24.62189639131861	24.780745977030254
43	22.851542241511392	24.991825854099524	27.352034796325558	24.804597108063525
44	23.219155038143384	25.908804437047493	27.165069307806483	23.706971217002646
45	21.578586844179455	27.71812069935939	26.340967976829127	24.36232447963203
46	24.26539306190079	26.66956600945305	26.022952896385526	23.04208803226063
47	23.024555081633522	25.954347987475263	27.264001482666334	23.75709544822488
48	22.95779297534172	24.06121053177492	28.98696927942732	23.99402721345604
49	23.941796922098415	22.983118348469763	28.283439891200935	24.79164483823089
50	21.7098996936209	26.035694560204625	27.982694503867517	24.271711242306957
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1024.0
1	1699.0
2	2374.0
3	8924.0
4	15474.0
5	10660.0
6	5846.0
7	6098.5
8	6351.0
9	6820.5
10	7290.0
11	7554.5
12	7819.0
13	7774.0
14	7729.0
15	7376.5
16	7024.0
17	7232.0
18	7440.0
19	7361.5
20	7283.0
21	7504.5
22	7726.0
23	8619.0
24	9512.0
25	10922.0
26	12332.0
27	14859.5
28	17387.0
29	19351.0
30	21315.0
31	25061.0
32	28807.0
33	32188.5
34	35570.0
35	40779.0
36	45988.0
37	52345.0
38	58702.0
39	71829.0
40	84956.0
41	102983.5
42	121011.0
43	140258.0
44	159505.0
45	170246.0
46	180987.0
47	173186.5
48	165386.0
49	152355.0
50	139324.0
51	128131.5
52	116939.0
53	110331.5
54	103724.0
55	99842.5
56	95961.0
57	93272.5
58	90584.0
59	85807.0
60	81030.0
61	76510.0
62	71990.0
63	63938.0
64	55886.0
65	47316.5
66	38747.0
67	33399.5
68	28052.0
69	23898.0
70	19744.0
71	16538.0
72	13332.0
73	10783.0
74	8234.0
75	6655.0
76	5076.0
77	3970.0
78	2864.0
79	2210.5
80	1557.0
81	1192.0
82	827.0
83	601.0
84	375.0
85	241.5
86	108.0
87	81.0
88	54.0
89	42.5
90	31.0
91	17.5
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1899281.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.644873452501162
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.91190091577393	20.98574894355377
2	6.987409340557342	3.8633209396106456
3	2.6619873282372	2.2077090846383722
4	1.676750826104953	1.854142575961928
5	1.1816802846787895	1.633370096563034
6	0.9645531862947917	1.5998970463995774
7	0.7635152351877947	1.4775097439076275
8	0.6619773094246069	1.4640223157976366
9	0.6004087569129355	1.4938401694168466
>10	8.018206880942472	50.92252398065309
>50	0.5167784351567279	8.809295828772118
>100	0.05035945569230231	2.1697549189294354
>500	0.0031109878512093295	0.6314222878414316
>1k	0.0013610571849040818	0.8874420679545447
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4894	0.25767645756473107	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4530	0.23851131033269957	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2804	0.14763481549070412	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06223407700071764	0.0
2	5.26515033847019E-5	0.0	0.0	0.20381396960218104	0.0
3	5.26515033847019E-5	0.0	0.0	0.31564576279128786	0.0
4	5.26515033847019E-5	0.0	0.0	0.5313589721584115	0.0
5	5.26515033847019E-5	0.0	0.0	0.871277078010047	0.0
6	5.26515033847019E-5	0.0	0.0	1.535212535691138	0.0
7	5.26515033847019E-5	0.0	0.0	1.8627575382473684	0.0
8	5.26515033847019E-5	0.0	0.0	2.8606614818976235	0.0
9	5.26515033847019E-5	0.0	0.0	3.739362421884913	0.0
10	5.26515033847019E-5	0.0	0.0	4.95150533280752	0.0
11	5.26515033847019E-5	0.0	0.0	5.757389243613767	0.0
12	5.26515033847019E-5	0.0	0.0	6.469448175388476	0.0
13	5.26515033847019E-5	0.0	0.0	6.817316658251201	0.0
14	5.26515033847019E-5	0.0	0.0	6.979746546193007	0.0
15	5.26515033847019E-5	0.0	0.0	7.153496507362523	0.0
16	5.26515033847019E-5	0.0	0.0	7.452872955607938	0.0
17	5.26515033847019E-5	0.0	0.0	7.768466066895841	0.0
18	5.26515033847019E-5	0.0	0.0	8.2156879366455	0.0
19	5.26515033847019E-5	0.0	0.0	8.424925011096304	0.0
20	5.26515033847019E-5	0.0	0.0	8.672702986024712	0.0
21	5.26515033847019E-5	0.0	0.0	8.923271490632509	0.0
22	5.26515033847019E-5	0.0	0.0	9.178683933551696	0.0
23	5.26515033847019E-5	0.0	0.0	9.457526295477077	0.0
24	5.26515033847019E-5	0.0	0.0	9.691193667498386	0.0
25	5.26515033847019E-5	0.0	0.0	9.882476579295007	0.0
26	5.26515033847019E-5	0.0	0.0	10.068020477222696	0.0
27	5.26515033847019E-5	0.0	0.0	10.266832554003331	0.0
28	5.26515033847019E-5	0.0	0.0	10.45753629926272	0.0
29	5.26515033847019E-5	0.0	0.0	10.654242315907966	0.0
30	5.26515033847019E-5	0.0	0.0	10.907917259215461	0.0
31	5.26515033847019E-5	0.0	0.0	11.118891833277962	0.0
32	5.26515033847019E-5	0.0	0.0	11.338606556902322	0.0
33	5.26515033847019E-5	0.0	0.0	11.546737949782049	0.0
34	5.26515033847019E-5	0.0	0.0	11.761450780584864	0.0
35	5.26515033847019E-5	0.0	0.0	11.986641260561234	0.0
36	5.26515033847019E-5	0.0	0.0	12.193140456836034	0.0
37	5.26515033847019E-5	0.0	0.0	12.408274499665927	0.0
38	5.26515033847019E-5	0.0	0.0	12.639046039001075	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACA	20	7.85778E-4	44.000004	22
TCTAGCG	65	0.0	44.000004	1
GAGCGAT	2050	0.0	40.887806	7
CGTTTTT	5695	0.0	40.755047	1
AGCGATA	380	0.0	40.526318	8
CTATCTC	1055	0.0	40.037914	6
AGACCGA	470	0.0	38.851063	8
TCACGAC	125	0.0	38.72	25
GTTTTTA	3410	0.0	37.741936	2
GCGATAA	175	0.0	37.714287	9
GATCGAC	35	7.2927196E-6	37.714283	9
TATCTCT	1170	0.0	36.8547	7
TATAGCG	90	0.0	36.666668	1
GACGATA	90	0.0	36.666668	7
TTATACG	30	1.3014152E-4	36.666664	1
TGATTCG	240	0.0	36.666664	15
TAACGAG	235	0.0	36.510635	3
ATTAGCG	85	0.0	36.23529	1
GAGACCG	630	0.0	35.968254	7
GTAGCAT	1055	0.0	35.658768	29
>>END_MODULE