FastQCFastQC Report
Thu 26 May 2016
SRR1546732_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546732_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3429701
Sequences flagged as poor quality0
Sequence length50
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247240.7208791670177662No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229850.6701750385820804No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT340800.042.8767621
GTTTTTA187450.041.4414522
GAGCGAT32100.040.6417477
AGCGATA7750.040.3096778
CTATCTC31600.039.8924066
CGTTAGA1800.039.111112
ATGTCTC39000.038.1333358
TATTTTT209000.037.4947367
CTATGCG1300.037.230771
TATCTCT33900.037.185847
TTATTTT211400.037.1210986
ACGTCGT301.3016337E-436.66666838
TTTATTT215400.036.646245
CGTTGAG4150.036.5783123
TTTTATT217200.036.5349924
TCGTTAG1150.036.3478281
GACCGAT19450.036.1953749
CGCAATC3100.036.1935520
AGACCGA10800.036.0555538
GCGATAT4400.036.09
Produced by FastQC (version 0.11.5)