FastQCFastQC Report
Thu 26 May 2016
SRR1546731_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546731_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1377110
Sequences flagged as poor quality0
Sequence length50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41000.2977249457196593No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37710.2738343342216671No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA16870.12250292278757689No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTACG408.314601E-944.01
CCGGTAT302.5280842E-644.042
TATACGA254.4433014E-544.02
CGTTTTT46700.040.7494621
GCCGATT1150.040.1739169
CGACGGT1700.040.1176528
CACGACG1700.040.1176526
GAGCGAT12600.039.6349227
TATGCGA900.039.111112
GCGATAA1500.038.133339
TAATGCG750.038.133331
TCACGAC1800.037.8888925
TTGTCGA357.291257E-637.7142872
CGTAAGA1000.037.3999982
CGTACAT651.0913936E-1137.23076635
CTATCTC5800.037.1724176
TAGGATC4500.037.15555634
GTTTTTA28250.037.0690272
AGCGATA3400.036.8823558
ACGTTGA601.9826984E-1036.6666642
Produced by FastQC (version 0.11.5)