##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546731_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1377110
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.499249878368467	31.0	31.0	33.0	30.0	34.0
2	31.876491347822615	31.0	31.0	34.0	30.0	34.0
3	31.99383781978201	33.0	31.0	34.0	30.0	34.0
4	35.688491115451924	37.0	35.0	37.0	33.0	37.0
5	35.626400941101295	37.0	35.0	37.0	33.0	37.0
6	35.65456644712478	37.0	35.0	37.0	33.0	37.0
7	36.01356826978237	37.0	35.0	37.0	35.0	37.0
8	19.067052014726492	33.0	0.0	37.0	0.0	37.0
9	28.23951536188104	33.0	17.0	39.0	17.0	39.0
10	33.67221572713872	33.0	32.0	39.0	27.0	39.0
11	35.77929214078759	37.0	35.0	39.0	32.0	39.0
12	36.478755509726895	37.0	35.0	39.0	32.0	39.0
13	36.69468088968927	39.0	35.0	39.0	33.0	39.0
14	37.825186804249476	40.0	36.0	41.0	33.0	41.0
15	38.02325086594389	40.0	37.0	41.0	33.0	41.0
16	38.093996122314124	40.0	37.0	41.0	33.0	41.0
17	38.05791476352652	40.0	37.0	41.0	33.0	41.0
18	38.01794555264286	40.0	36.0	41.0	33.0	41.0
19	38.021358497142565	40.0	37.0	41.0	33.0	41.0
20	37.95875855959219	40.0	36.0	41.0	33.0	41.0
21	37.801110296200015	40.0	36.0	41.0	33.0	41.0
22	37.796953039336	40.0	36.0	41.0	33.0	41.0
23	37.66775275758654	39.0	36.0	41.0	33.0	41.0
24	37.53958797772146	39.0	35.0	41.0	33.0	41.0
25	37.376792703560355	39.0	35.0	41.0	32.0	41.0
26	37.35505369941399	39.0	35.0	41.0	32.0	41.0
27	37.220319364466164	39.0	35.0	41.0	32.0	41.0
28	37.22194305465794	39.0	35.0	41.0	32.0	41.0
29	37.132109998475066	39.0	35.0	41.0	32.0	41.0
30	37.02501180007407	39.0	35.0	41.0	32.0	41.0
31	37.02965558306889	39.0	35.0	41.0	31.0	41.0
32	36.83341708360262	39.0	35.0	41.0	31.0	41.0
33	36.759904437554006	39.0	35.0	41.0	31.0	41.0
34	36.60031297427221	39.0	35.0	41.0	30.0	41.0
35	36.43072957134869	39.0	35.0	41.0	30.0	41.0
36	36.293139981555576	39.0	35.0	41.0	30.0	41.0
37	36.24254053779291	39.0	35.0	41.0	30.0	41.0
38	36.163304311202445	39.0	35.0	41.0	30.0	41.0
39	36.07288088823696	39.0	35.0	40.0	30.0	41.0
40	35.99488784483447	39.0	35.0	40.0	29.0	41.0
41	35.961548460181106	39.0	35.0	40.0	29.0	41.0
42	35.78080400258512	38.0	35.0	40.0	28.0	41.0
43	35.75374661428644	38.0	35.0	40.0	28.0	41.0
44	35.46961172310128	38.0	35.0	40.0	27.0	41.0
45	35.35824589175883	38.0	34.0	40.0	27.0	41.0
46	35.34213897219539	38.0	34.0	40.0	27.0	41.0
47	35.204097711874866	38.0	34.0	40.0	26.0	41.0
48	35.16694526944108	38.0	34.0	40.0	26.0	41.0
49	35.114070771398076	37.0	34.0	40.0	27.0	41.0
50	34.48974519101597	37.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	6.0
13	4.0
14	12.0
15	27.0
16	61.0
17	142.0
18	365.0
19	837.0
20	1527.0
21	2612.0
22	4109.0
23	6237.0
24	8852.0
25	12590.0
26	16328.0
27	19353.0
28	22175.0
29	26010.0
30	31494.0
31	40727.0
32	52532.0
33	69278.0
34	103087.0
35	149801.0
36	151062.0
37	222185.0
38	233696.0
39	201999.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.69796167335943	24.250205139749184	28.090203396969017	13.961629789922373
2	32.44526581028386	25.47944608636928	28.23601600453123	13.839272098815636
3	29.599741487608107	25.99196868804961	30.718098045907734	13.69019177843455
4	25.501593917697207	28.703662016832354	30.195554458249525	15.59918960722092
5	23.78945763228791	31.56523444024079	29.600177182650622	15.045130744820675
6	21.650194973531526	40.19896740274923	26.779124398196224	11.371713225523015
7	85.63818431352615	4.4543282671682	7.5965609137977355	2.3109265055079113
8	45.29442092498058	49.080247765247506	3.7113229879966014	1.9140083217753119
9	79.41275569852809	5.77840550137607	10.429304848559665	4.379533951536188
10	43.51054018923688	24.894525491790777	16.43579670469316	15.159137614279178
11	34.18434257248876	25.952102591659344	23.658531272011675	16.205023563840214
12	29.94764397905759	23.29044157692559	27.817966611236578	18.943947832780243
13	24.067285837732644	28.494746243945656	29.67199424882544	17.765973669496262
14	20.552025618868498	30.364967213948052	30.16251425085868	18.920492916324765
15	18.997247859648102	23.619318718185184	38.289679110601185	19.093754311565526
16	21.61773569286404	22.126917965885077	35.936853265171266	20.318493076079616
17	22.024457015053265	23.031203026628226	29.44165680301501	25.502683155303497
18	23.838473324571023	23.035269513691716	31.876829011480563	21.249428150256698
19	27.69531845676816	25.680882427692776	26.806645801715185	19.817153313823876
20	28.94068011996137	24.976363543943474	25.45359484717996	20.629361488915194
21	25.54785020804438	27.245753788731474	27.487128842285657	19.719267160938486
22	24.503198727770474	24.448155920732546	26.953257183522012	24.095388167974964
23	22.977975615600787	27.726761115669774	26.08077786088257	23.21448540784687
24	22.681920834210775	24.624685028792182	31.879733645097343	20.813660491899704
25	22.32639367951725	25.45620901743506	29.619347764521354	22.598049538526336
26	21.055689088017658	28.56155281713153	27.928633152035786	22.454124942815028
27	21.4602319349943	27.29193746323823	28.61695870337155	22.630871898395917
28	21.066508848240158	26.686829665023126	31.531758537807438	20.71490294892928
29	22.384631583533633	24.785964810363733	30.23062790917211	22.598775696930527
30	24.29348418063916	25.788862182396464	28.908946997698077	21.00870663926629
31	27.613189941253786	25.029155259928398	25.540298160640763	21.817356638177053
32	28.116272483679587	24.935916520829853	26.422653237577244	20.525157757913313
33	27.459462207085856	25.177001111022356	26.038805905120142	21.324730776771645
34	23.730130490665232	25.34329138558285	29.557624300164836	21.368953823587077
35	24.021392626587566	25.33051099766903	29.670759779538308	20.977336596205095
36	26.60666177720008	25.3703770940593	28.07473622295968	19.948224905780947
37	24.47357146487935	27.184466019417474	28.16289185322886	20.179070662474313
38	23.448163182316588	27.7814408435056	27.799740035291297	20.97065593888651
39	23.118559882652804	26.525041572568643	27.368111479838213	22.988287064940348
40	24.413082469809964	24.249188517983313	28.361133097573905	22.97659591463282
41	21.500533726427083	23.87078737355767	28.36701498064788	26.261663919367372
42	24.009774092120455	25.19058027318079	26.87592131347532	23.923724321223432
43	23.21920543747413	24.63986173943984	28.238049248062975	23.902883575023058
44	22.64437844471393	26.526276041855773	28.4810944659468	22.348251047483497
45	21.97682102373812	27.16210034056829	27.41661886123839	23.4444597744552
46	23.639869001023882	26.715585537829224	27.567296730108705	22.07724873103819
47	22.8087080915831	25.965318674615677	28.554291233089586	22.671682000711634
48	23.28274429784113	24.114413518164852	29.237896754798093	23.364945429195924
49	23.41265403635149	23.42151316888266	29.326633311790633	23.839199482975214
50	21.70037251926135	26.70825133794686	28.51950824552868	23.07186789726311
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	575.0
1	1142.5
2	1710.0
3	7364.0
4	13018.0
5	9082.5
6	5147.0
7	5255.5
8	5364.0
9	5700.0
10	6036.0
11	6052.5
12	6069.0
13	6139.0
14	6209.0
15	6142.5
16	6076.0
17	5995.0
18	5914.0
19	5895.5
20	5877.0
21	6366.5
22	6856.0
23	7590.0
24	8324.0
25	9876.5
26	11429.0
27	13105.5
28	14782.0
29	16726.5
30	18671.0
31	21632.0
32	24593.0
33	27724.0
34	30855.0
35	35011.5
36	39168.0
37	44755.0
38	50342.0
39	58956.5
40	67571.0
41	75074.0
42	82577.0
43	89966.0
44	97355.0
45	105207.0
46	113059.0
47	116689.5
48	120320.0
49	117391.5
50	114463.0
51	104337.0
52	94211.0
53	85363.5
54	76516.0
55	71003.0
56	65490.0
57	63176.5
58	60863.0
59	57634.5
60	54406.0
61	50908.0
62	47410.0
63	42266.5
64	37123.0
65	31499.5
66	25876.0
67	22488.0
68	19100.0
69	16092.5
70	13085.0
71	10978.5
72	8872.0
73	7124.0
74	5376.0
75	4156.5
76	2937.0
77	2281.0
78	1625.0
79	1266.5
80	908.0
81	759.5
82	611.0
83	428.5
84	246.0
85	160.0
86	74.0
87	42.5
88	11.0
89	8.0
90	5.0
91	4.0
92	3.0
93	2.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1377110.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.82554660853088
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.19698286788342	21.831400241298933
2	6.653108401908795	3.968651894654783
3	3.0675793389917176	2.7447669165139144
4	2.027431975914772	2.4187706757308756
5	1.54404276197873	2.302595968148068
6	1.2816958281755033	2.2936367196724836
7	1.0501925946932347	2.1925797726669938
8	0.9709950139496288	2.316836563616459
9	0.8663398325839403	2.325515315000324
>10	9.221439933103895	53.81995690072198
>50	0.10045062943769355	1.8953755861672559
>100	0.018278538539866582	1.0712175210762707
>500	7.311414193929938E-4	0.12393470227004678
>1k	7.311414193929938E-4	0.6947612224616465
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4100	0.2977249457196593	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3771	0.2738343342216671	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1687	0.12250292278757689	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04669198538969291	0.0
2	0.0	0.0	0.0	0.15968223308232457	0.0
3	0.0	0.0	0.0	0.24667600990480062	0.0
4	0.0	0.0	0.0	0.37876422362774215	0.0
5	0.0	0.0	0.0	0.591964331099186	0.0
6	0.0	0.0	0.0	1.0903994597381472	0.0
7	0.0	0.0	0.0	1.3443370536848909	0.0
8	0.0	0.0	0.0	2.103680896950861	0.0
9	0.0	0.0	0.0	2.839860287123033	0.0
10	0.0	0.0	0.0	3.869407672589699	0.0
11	0.0	0.0	0.0	4.563034180276086	0.0
12	0.0	0.0	0.0	5.165600424076508	0.0
13	0.0	0.0	0.0	5.49091938915555	0.0
14	0.0	0.0	0.0	5.646026824291451	0.0
15	0.0	0.0	0.0	5.788353871513532	0.0
16	7.26158404194291E-5	0.0	0.0	6.037208356630916	0.0
17	7.26158404194291E-5	0.0	0.0	6.293324425790242	0.0
18	7.26158404194291E-5	0.0	0.0	6.646237410228667	0.0
19	7.26158404194291E-5	0.0	0.0	6.822040359884105	0.0
20	7.26158404194291E-5	0.0	0.0	7.02623610314354	0.0
21	7.26158404194291E-5	0.0	0.0	7.235224491870657	0.0
22	7.26158404194291E-5	0.0	0.0	7.440436856895963	0.0
23	7.26158404194291E-5	0.0	0.0	7.670411223504296	0.0
24	7.26158404194291E-5	0.0	0.0	7.866837071838851	0.0
25	7.26158404194291E-5	0.0	0.0	8.020927885208879	0.0
26	7.26158404194291E-5	0.0	0.0	8.176543631227716	0.0
27	2.1784752125828728E-4	0.0	0.0	8.360915250052647	0.0
28	2.1784752125828728E-4	0.0	0.0	8.514207289178062	0.0
29	2.1784752125828728E-4	0.0	0.0	8.675051375707097	0.0
30	2.1784752125828728E-4	0.0	0.0	8.890357342550704	0.0
31	2.1784752125828728E-4	0.0	0.0	9.055485763664485	0.0
32	2.1784752125828728E-4	0.0	0.0	9.24704635069094	0.0
33	2.1784752125828728E-4	0.0	0.0	9.427424098292802	0.0
34	2.1784752125828728E-4	0.0	0.0	9.599886719288946	0.0
35	2.1784752125828728E-4	0.0	0.0	9.781644167858778	0.0
36	2.1784752125828728E-4	0.0	0.0	9.94924152754682	0.0
37	2.1784752125828728E-4	0.0	0.0	10.126787257372323	0.0
38	2.1784752125828728E-4	0.0	0.0	10.33192700655721	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATTACG	40	8.314601E-9	44.0	1
CCGGTAT	30	2.5280842E-6	44.0	42
TATACGA	25	4.4433014E-5	44.0	2
CGTTTTT	4670	0.0	40.749462	1
GCCGATT	115	0.0	40.173916	9
CGACGGT	170	0.0	40.11765	28
CACGACG	170	0.0	40.11765	26
GAGCGAT	1260	0.0	39.634922	7
TATGCGA	90	0.0	39.11111	2
GCGATAA	150	0.0	38.13333	9
TAATGCG	75	0.0	38.13333	1
TCACGAC	180	0.0	37.88889	25
TTGTCGA	35	7.291257E-6	37.714287	2
CGTAAGA	100	0.0	37.399998	2
CGTACAT	65	1.0913936E-11	37.230766	35
CTATCTC	580	0.0	37.172417	6
TAGGATC	450	0.0	37.155556	34
GTTTTTA	2825	0.0	37.069027	2
AGCGATA	340	0.0	36.882355	8
ACGTTGA	60	1.9826984E-10	36.666664	2
>>END_MODULE