FastQCFastQC Report
Thu 26 May 2016
SRR1546729_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546729_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1294517
Sequences flagged as poor quality0
Sequence length50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67650.5225887338675352No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59890.46264359602848015No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG302.527966E-644.01
TACGTAG750.044.01
CGTTTTT63200.041.354431
TATGCGA950.039.3684232
GAGCGAT12300.039.3495947
CACGACG452.3514076E-839.11110726
AGCCGAT1700.038.8235328
GTTTTTA35750.038.6461522
GCGATCG1100.038.09
GCCGATA1350.037.481489
TAATCGT1300.037.2307721
CTATCTC6150.037.203256
TATTACG301.3011861E-436.6666641
AGCGATA2550.036.2352948
AGACCGA4800.035.758
ACATACG1600.035.7517
GAGACCG5750.035.582617
TGTATCG250.002352854535.21
TGTAACG505.94664E-835.21
TAATCCG250.002352854535.21
Produced by FastQC (version 0.11.5)