##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546729_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1294517
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.46216388042799	31.0	31.0	33.0	30.0	34.0
2	31.846352732331827	31.0	31.0	34.0	30.0	34.0
3	31.95578350844369	31.0	31.0	34.0	30.0	34.0
4	35.660696615030936	37.0	35.0	37.0	33.0	37.0
5	35.60563206199687	37.0	35.0	37.0	33.0	37.0
6	35.633954594648046	37.0	35.0	37.0	33.0	37.0
7	35.99441027039428	37.0	35.0	37.0	35.0	37.0
8	18.97892495811179	33.0	0.0	37.0	0.0	37.0
9	28.18032517147322	32.0	17.0	39.0	17.0	39.0
10	33.63858952798611	33.0	32.0	39.0	27.0	39.0
11	35.744439045605425	37.0	35.0	39.0	32.0	39.0
12	36.46028132500384	37.0	35.0	39.0	32.0	39.0
13	36.70046279809381	39.0	35.0	39.0	33.0	39.0
14	37.79761640828201	39.0	36.0	41.0	33.0	41.0
15	37.99442185772763	40.0	37.0	41.0	33.0	41.0
16	38.06187867752992	40.0	37.0	41.0	33.0	41.0
17	38.01627634090553	40.0	37.0	41.0	33.0	41.0
18	37.97692884682086	40.0	36.0	41.0	33.0	41.0
19	37.97342560970617	40.0	37.0	41.0	33.0	41.0
20	37.879825448410486	40.0	36.0	41.0	33.0	41.0
21	37.729792656257125	40.0	36.0	41.0	33.0	41.0
22	37.72547830580827	40.0	36.0	41.0	33.0	41.0
23	37.54826626456045	39.0	36.0	41.0	32.0	41.0
24	37.401425396499235	39.0	35.0	41.0	32.0	41.0
25	37.22940061814561	39.0	35.0	41.0	32.0	41.0
26	37.21122009212702	39.0	35.0	41.0	32.0	41.0
27	37.07454981278732	39.0	35.0	41.0	32.0	41.0
28	37.052735499031684	39.0	35.0	41.0	32.0	41.0
29	36.938860594337505	39.0	35.0	40.0	31.0	41.0
30	36.80621807206858	39.0	35.0	40.0	31.0	41.0
31	36.795974096902555	39.0	35.0	41.0	31.0	41.0
32	36.611329940047135	39.0	35.0	41.0	30.0	41.0
33	36.450992918594345	39.0	35.0	41.0	30.0	41.0
34	36.2785208691736	39.0	35.0	41.0	30.0	41.0
35	36.07952155128129	39.0	35.0	41.0	29.0	41.0
36	35.929983152017314	39.0	35.0	40.0	29.0	41.0
37	35.88206489370167	39.0	35.0	40.0	29.0	41.0
38	35.77465185856964	38.0	35.0	40.0	28.0	41.0
39	35.68165423860791	38.0	35.0	40.0	27.0	41.0
40	35.584419517086296	38.0	35.0	40.0	27.0	41.0
41	35.53550552059185	38.0	35.0	40.0	27.0	41.0
42	35.354229415295436	38.0	35.0	40.0	26.0	41.0
43	35.33157849607228	38.0	34.0	40.0	27.0	41.0
44	35.050530043251655	38.0	34.0	40.0	26.0	41.0
45	34.936020925179044	37.0	34.0	40.0	25.0	41.0
46	34.91588909222513	37.0	34.0	40.0	26.0	41.0
47	34.761638510734116	37.0	34.0	40.0	25.0	41.0
48	34.71631118015445	37.0	34.0	40.0	25.0	41.0
49	34.66144283929837	37.0	34.0	40.0	25.0	41.0
50	34.047819379737774	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	2.0
12	2.0
13	8.0
14	8.0
15	25.0
16	60.0
17	177.0
18	395.0
19	849.0
20	1558.0
21	2666.0
22	4235.0
23	6200.0
24	9254.0
25	13449.0
26	18016.0
27	21437.0
28	23406.0
29	26760.0
30	32241.0
31	40280.0
32	51612.0
33	67695.0
34	99278.0
35	138345.0
36	146951.0
37	206716.0
38	208461.0
39	174429.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.58504909553138	24.644713047414594	27.495660543662233	15.274577313391791
2	33.24444561176099	25.35895627481138	27.58349253041868	13.813105583008953
3	29.26157014546738	25.285260834736047	31.578032578946434	13.875136440850138
4	25.53886893721751	27.877579050719305	30.899632836030737	15.683919176032452
5	23.614753610806194	30.81519980038887	30.36514777326215	15.204898815542785
6	21.888704435708455	39.548804689316555	27.3181425968141	11.24434827816089
7	84.4810844508029	4.177465417603631	9.088177289290137	2.2532728423033457
8	44.48408170769484	49.05335349014342	4.553281262432243	1.9092835397294898
9	78.31283791560868	5.455625534465751	11.606722816309095	4.6248137336164765
10	42.30883024324903	25.290900003630696	17.354349151073333	15.045920602046941
11	33.816705381234854	24.34390587377377	25.072826390074443	16.766562354916932
12	28.86667382506371	21.562096133152366	29.688679252570648	19.882550789213273
13	24.556031322879498	25.367221905930936	31.43334540990964	18.64340136127992
14	20.75901668344255	27.539769659262873	32.31939016637093	19.38182349092364
15	19.816734735812663	22.32940934727006	38.427305319281245	19.42655059763603
16	22.982703201271207	21.519300248664173	34.99954036911064	20.498456180953976
17	23.072080165807016	22.282982765000384	29.900032212786698	24.744904856405903
18	25.029180767807606	22.00805396916379	31.729517650212397	21.233247612816207
19	27.889089135175517	24.58291393624031	27.306169019024086	20.22182790956009
20	29.17026195870738	23.967472037833414	26.541250520464388	20.321015482994817
21	25.990465942123585	25.961420359871674	28.265291224448962	19.782822473555775
22	25.260618439155298	22.8087387033156	28.117436850964488	23.81320600656461
23	23.957120686711722	25.886566186461824	27.1554564366478	23.000856690178654
24	23.514716299592823	23.775894793193135	31.23751947637613	21.471869430837913
25	24.00910918898709	24.207716082523444	29.13673594089533	22.646438787594136
26	22.075028755898916	25.98644899989726	28.31828396228091	23.62023828192291
27	22.531569689698937	24.727832851944008	29.418076394516255	23.3225210638408
28	22.277034600549857	25.248335865809413	30.85984965821229	21.614779875428443
29	23.436308677290448	24.17627578471353	29.75928473708727	22.628130800908757
30	25.016975443350688	23.69872315311425	29.60694992804266	21.677351475492404
31	26.764886054026327	23.694551713109988	27.361401974636102	22.179160258227586
32	28.939596776249367	22.99058258794593	26.615718449429398	21.454102186375305
33	27.557459654836514	23.188648739259506	27.270711778987838	21.98317982691614
34	23.65600451751503	24.062488171263876	30.435985004445676	21.845522306775422
35	23.880335291077674	24.72914608305646	29.004717589649264	22.385801036216595
36	26.386907240306616	24.619761656277976	27.619336014899766	21.373995088515642
37	24.411035158286836	25.918856222050383	26.90771924972789	22.762389369934887
38	24.116794140208277	26.19293528010833	26.26755770685128	23.422712872832108
39	23.582231828550725	24.68426447856614	26.063079897753372	25.670423795129764
40	24.69206661635189	22.96354547680718	27.591912659316176	24.752475247524753
41	21.69117902661765	22.86590288115181	27.663213383833508	27.77970470839703
42	24.166928669148415	24.148543433574066	26.723326151761622	24.961201745515897
43	23.14747508143964	23.35056241053613	28.7813910516432	24.72057145638103
44	23.905209433325325	24.42424471830034	28.58293865588478	23.087607192489553
45	22.552581387498194	25.239297745800172	27.840113339569893	24.36800752713174
46	23.756119077617367	24.941039785495285	27.75915650393158	23.543684632955767
47	23.12298718363683	24.745909091962485	28.46389811798532	23.667205606415365
48	23.567091046313028	23.480726788446965	29.07470508305414	23.877477082185866
49	24.057157997925096	22.69850453875847	28.846898109487938	24.397439353828492
50	22.229294787167724	24.768620265319033	28.882046353968317	24.12003859354493
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	485.0
1	966.5
2	1448.0
3	10368.0
4	19288.0
5	12820.5
6	6353.0
7	6325.5
8	6298.0
9	6600.5
10	6903.0
11	6901.0
12	6899.0
13	6882.5
14	6866.0
15	6541.0
16	6216.0
17	6072.5
18	5929.0
19	5885.0
20	5841.0
21	6018.0
22	6195.0
23	6801.0
24	7407.0
25	8456.0
26	9505.0
27	10731.5
28	11958.0
29	13838.0
30	15718.0
31	18001.5
32	20285.0
33	22552.0
34	24819.0
35	28363.5
36	31908.0
37	35235.5
38	38563.0
39	44400.5
40	50238.0
41	57212.0
42	64186.0
43	69268.0
44	74350.0
45	78618.0
46	82886.0
47	90048.0
48	97210.0
49	102695.5
50	108181.0
51	104808.5
52	101436.0
53	92958.5
54	84481.0
55	79757.0
56	75033.0
57	71617.0
58	68201.0
59	64268.5
60	60336.0
61	57108.5
62	53881.0
63	48139.5
64	42398.0
65	36422.5
66	30447.0
67	26229.0
68	22011.0
69	18839.5
70	15668.0
71	13057.5
72	10447.0
73	8458.5
74	6470.0
75	5115.0
76	3760.0
77	2943.5
78	2127.0
79	1618.5
80	1110.0
81	783.5
82	457.0
83	330.0
84	203.0
85	126.0
86	49.0
87	43.0
88	37.0
89	24.5
90	12.0
91	11.0
92	10.0
93	8.0
94	6.0
95	4.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1294517.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.165143052301076
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.04858597942766	24.63268546577985
2	7.4097641366911615	5.211308317011098
3	3.940095758204317	4.156620929310584
4	2.8284595298425477	3.9785273593822974
5	2.264823481808263	3.9821420862999086
6	1.9148241040772456	4.0401038123922355
7	1.5746656601263502	3.876134023852245
8	1.4091199355723159	3.9641523289799805
9	1.2197497213159345	3.860340609427129
>10	7.327882417927317	38.94061801711494
>50	0.042767767326420604	0.9851432335520827
>100	0.01793312875465361	1.1865792873904626
>500	8.855859505316931E-4	0.19268961918249666
>1k	0.0	0.0
>5k	4.4279297526584655E-4	0.9929549103246716
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6765	0.5225887338675352	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5989	0.46264359602848015	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04967103560633039	0.0
2	0.0	0.0	0.0	0.15735598682751945	0.0
3	0.0	0.0	0.0	0.25314460914765896	0.0
4	0.0	0.0	0.0	0.3948963204036718	0.0
5	0.0	0.0	0.0	0.641474773989063	0.0
6	0.0	0.0	0.0	1.1853069523227582	0.0
7	0.0	0.0	0.0	1.476226268175698	0.0
8	0.0	0.0	0.0	2.230870664502668	0.0
9	0.0	0.0	0.0	3.033641118656611	0.0
10	0.0	0.0	0.0	4.114816568650701	0.0
11	0.0	0.0	0.0	4.826356084933608	0.0
12	0.0	0.0	0.0	5.413602138867238	0.0
13	0.0	0.0	0.0	5.73070882808028	0.0
14	0.0	0.0	0.0	5.896948437139103	0.0
15	0.0	0.0	0.0	6.046888530625708	0.0
16	0.0	0.0	0.0	6.282343144199729	0.0
17	0.0	0.0	0.0	6.522278193333884	0.0
18	0.0	0.0	0.0	6.882335264813054	0.0
19	0.0	0.0	0.0	7.059930460550151	0.0
20	7.724888896785442E-5	0.0	0.0	7.267729971873679	0.0
21	7.724888896785442E-5	0.0	0.0	7.472285029860558	0.0
22	7.724888896785442E-5	0.0	0.0	7.680084541184087	0.0
23	7.724888896785442E-5	0.0	0.0	7.910054483641389	0.0
24	7.724888896785442E-5	0.0	0.0	8.103562950505864	0.0
25	7.724888896785442E-5	0.0	0.0	8.265090377337648	0.0
26	7.724888896785442E-5	0.0	0.0	8.430789244173695	0.0
27	7.724888896785442E-5	0.0	0.0	8.624761204371978	0.0
28	7.724888896785442E-5	0.0	0.0	8.793318280099836	0.0
29	7.724888896785442E-5	0.0	0.0	8.972303955838354	0.0
30	7.724888896785442E-5	0.0	0.0	9.187982853836605	0.0
31	7.724888896785442E-5	0.0	0.0	9.375156911805716	0.0
32	7.724888896785442E-5	0.0	0.0	9.582801925351308	0.0
33	7.724888896785442E-5	0.0	0.0	9.778396112217916	0.0
34	7.724888896785442E-5	0.0	0.0	9.97375855241762	0.0
35	7.724888896785442E-5	0.0	0.0	10.169352739284227	0.0
36	7.724888896785442E-5	0.0	0.0	10.357299286143016	0.0
37	7.724888896785442E-5	0.0	0.0	10.55915063301602	0.0
38	1.5449777793570885E-4	0.0	0.0	10.797772451037723	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAACG	30	2.527966E-6	44.0	1
TACGTAG	75	0.0	44.0	1
CGTTTTT	6320	0.0	41.35443	1
TATGCGA	95	0.0	39.368423	2
GAGCGAT	1230	0.0	39.349594	7
CACGACG	45	2.3514076E-8	39.111107	26
AGCCGAT	170	0.0	38.823532	8
GTTTTTA	3575	0.0	38.646152	2
GCGATCG	110	0.0	38.0	9
GCCGATA	135	0.0	37.48148	9
TAATCGT	130	0.0	37.23077	21
CTATCTC	615	0.0	37.20325	6
TATTACG	30	1.3011861E-4	36.666664	1
AGCGATA	255	0.0	36.235294	8
AGACCGA	480	0.0	35.75	8
ACATACG	160	0.0	35.75	17
GAGACCG	575	0.0	35.58261	7
TGTATCG	25	0.0023528545	35.2	1
TGTAACG	50	5.94664E-8	35.2	1
TAATCCG	25	0.0023528545	35.2	1
>>END_MODULE