FastQCFastQC Report
Thu 26 May 2016
SRR1546728_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546728_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2156912
Sequences flagged as poor quality0
Sequence length50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123310.5716969445206851No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112910.523479863805292No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAG850.044.01
CGTTTTT115300.041.5958371
GTTTTTA62550.040.342132
GTATACG557.8216544E-1140.01
CTATCTC12400.039.5645186
TTAGCGA2300.039.2173922
GAGCGAT21750.038.9425287
TACGTAG800.038.5000041
TATGCGA750.038.133332
CGTAAGG2500.037.842
TATCTCT13050.037.593877
TATACGA651.0913936E-1137.2307662
TATACTA10950.036.96803744
ATGTCTC17950.036.8913658
TAAGACG601.9826984E-1036.6666681
ATTAGCG1450.036.4137951
ACGTAAG1050.035.619051
CGTAAGA1800.035.4444432
GTTACAC4100.035.41463533
AACCGTA250.00235320135.241
Produced by FastQC (version 0.11.5)