FastQCFastQC Report
Thu 26 May 2016
SRR1546727_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546727_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1259602
Sequences flagged as poor quality0
Sequence length50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36040.2861221242900535No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33220.2637341001363923No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA16450.1305968075630239No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG254.443083E-544.01
TATTCCG207.8567694E-444.01
TATCGAT207.8567694E-444.013
CGTTTTT38800.040.768041
GAGCGAT11400.040.3333327
AGCGATC1650.040.08
CGTTCGA452.3512257E-839.11111514
CGTAAGA850.038.823532
AGCGATA2150.037.8604668
TTAGACG700.037.7142871
GTAACGA357.290764E-637.7142872
CTATCTC4300.037.3488356
ACGAGTA651.0913936E-1137.230775
TACGTTC301.301166E-436.66666839
TAGACGA1450.036.4137952
GTTTTTA21850.036.3478242
CGCGACA250.002352829835.216
TCGATCC250.002352829835.234
TATTGCG751.8189894E-1235.21
GACCGAT8550.034.9941529
Produced by FastQC (version 0.11.5)