##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546727_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1259602
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.57378600542076	31.0	31.0	33.0	30.0	34.0
2	31.96975314424715	31.0	31.0	34.0	30.0	34.0
3	32.097709435202546	33.0	31.0	34.0	30.0	34.0
4	35.776084826794495	37.0	35.0	37.0	35.0	37.0
5	35.72710268799192	37.0	35.0	37.0	35.0	37.0
6	35.75731778768214	37.0	35.0	37.0	35.0	37.0
7	36.05407263564205	37.0	35.0	37.0	35.0	37.0
8	19.051104237687777	33.0	0.0	37.0	0.0	37.0
9	28.263668206306438	33.0	17.0	39.0	17.0	39.0
10	33.78774247738571	34.0	32.0	39.0	27.0	39.0
11	35.885436034556946	37.0	35.0	39.0	32.0	39.0
12	36.61285469537203	37.0	35.0	39.0	33.0	39.0
13	36.82213826272108	39.0	35.0	39.0	33.0	39.0
14	37.974371269655016	40.0	37.0	41.0	33.0	41.0
15	38.18474724555852	40.0	37.0	41.0	33.0	41.0
16	38.25013456631539	40.0	37.0	41.0	34.0	41.0
17	38.22428433743357	40.0	37.0	41.0	34.0	41.0
18	38.18993221668432	40.0	37.0	41.0	33.0	41.0
19	38.19818006005072	40.0	37.0	41.0	34.0	41.0
20	38.13159474183115	40.0	37.0	41.0	34.0	41.0
21	37.99187600527786	40.0	36.0	41.0	33.0	41.0
22	37.97354481812509	40.0	36.0	41.0	33.0	41.0
23	37.841009302938545	40.0	36.0	41.0	33.0	41.0
24	37.703846135525346	40.0	36.0	41.0	33.0	41.0
25	37.53385355056597	39.0	35.0	41.0	33.0	41.0
26	37.52455775713281	39.0	35.0	41.0	33.0	41.0
27	37.38612037770661	39.0	35.0	41.0	33.0	41.0
28	37.37741921654618	39.0	35.0	41.0	33.0	41.0
29	37.2890841710318	39.0	35.0	41.0	32.0	41.0
30	37.18919468212975	39.0	35.0	41.0	32.0	41.0
31	37.2404640513432	39.0	35.0	41.0	32.0	41.0
32	37.07919565068966	39.0	35.0	41.0	32.0	41.0
33	37.00923148740634	39.0	35.0	41.0	31.0	41.0
34	36.884546864803326	39.0	35.0	41.0	31.0	41.0
35	36.70376039415625	39.0	35.0	41.0	31.0	41.0
36	36.57925360550396	39.0	35.0	41.0	31.0	41.0
37	36.45261677895081	39.0	35.0	41.0	30.0	41.0
38	36.36921980117529	39.0	35.0	41.0	30.0	41.0
39	36.2433554408456	39.0	35.0	41.0	30.0	41.0
40	36.165545942289704	39.0	35.0	40.0	30.0	41.0
41	36.13973064507678	39.0	35.0	40.0	30.0	41.0
42	35.98299859796983	38.0	35.0	40.0	30.0	41.0
43	35.98086379665958	38.0	35.0	40.0	30.0	41.0
44	35.74316172886356	38.0	35.0	40.0	29.0	41.0
45	35.633807345494844	38.0	35.0	40.0	29.0	41.0
46	35.61845408311514	38.0	35.0	40.0	29.0	41.0
47	35.47816056182826	38.0	34.0	40.0	28.0	41.0
48	35.44226112692739	38.0	34.0	40.0	28.0	41.0
49	35.38825994242626	37.0	34.0	40.0	28.0	41.0
50	34.76012819922484	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	4.0
12	4.0
13	8.0
14	12.0
15	14.0
16	54.0
17	121.0
18	278.0
19	554.0
20	1098.0
21	1868.0
22	3104.0
23	4544.0
24	6655.0
25	9818.0
26	12789.0
27	15466.0
28	17620.0
29	21192.0
30	26347.0
31	34543.0
32	45829.0
33	61910.0
34	95033.0
35	139771.0
36	144480.0
37	210646.0
38	218674.0
39	187164.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.958586918725125	24.60475610549999	28.862053251741422	14.574603724033464
2	30.541710794362032	26.2166938445636	29.97788190237869	13.263713458695683
3	29.230264797928236	25.48932123003933	31.306714343102026	13.973699628930408
4	25.927316723854045	28.08220374372222	30.387296939827024	15.60318259259671
5	23.70359843823684	31.38737474218047	29.730819735122683	15.178207084460013
6	21.973448756035637	40.368941935627284	26.4480367608181	11.20957254751898
7	86.45572172797439	4.302867096114487	7.011262287611483	2.2301488882996376
8	45.87830124118571	49.05231970098492	3.3657456879236456	1.703633369905732
9	81.27495828047272	5.228238761132484	9.293491118623184	4.203311839771611
10	44.70165973061332	24.308710211638278	15.730524403740228	15.25910565400817
11	34.489545110280865	25.479318070311095	23.330067751559618	16.701069067848415
12	30.68119929946126	21.634611567780933	28.00781516701307	19.676373965744734
13	24.20907556513883	27.260277452719194	29.701921718130013	18.828725264011968
14	21.35206200053668	29.39912766095957	29.84053693150694	19.408273406996813
15	19.601270877626426	22.95828364832701	37.75240115528556	19.688044318761005
16	21.937643795421096	21.275688669913194	35.508358989585595	21.27830854508011
17	22.928353559298888	21.881514954723794	28.430329580296	26.759801905681318
18	24.132543454202203	22.80903015396927	31.247012945358932	21.8114134464696
19	28.50352730465655	24.226858960211242	26.061883039245732	21.20773069588648
20	29.314894704835336	23.706853434656345	25.20986787890143	21.76838398160689
21	26.47360039123469	25.895640051381307	27.308149717132874	20.322609840251125
22	25.29981692629894	23.519810225769728	26.476379046714754	24.703993801216576
23	24.253454662663284	26.838556941002	25.27568231870067	23.63230607763405
24	23.62222352774924	24.02433467079284	30.730659367006403	21.62278243445152
25	23.25933112205284	24.908264674079593	28.97327886110057	22.859125342767
26	22.0106827394685	27.539016292447933	27.391588771691378	23.058712196392193
27	22.66422250837963	26.33077749955938	27.666437493748024	23.33856249831296
28	21.71900330421832	25.2559935598705	31.064812536023283	21.960190599887902
29	22.30013925033463	24.134290037646814	30.09228311800077	23.473287594017794
30	23.96796765962582	25.071173275367936	29.035123793071143	21.925735271935103
31	27.50305255152024	24.386512565080082	24.829112687976043	23.281322195423634
32	28.543063602630035	24.30386741208731	25.519489489537172	21.63357949574548
33	27.781156269996394	24.099834709694015	25.273538784473192	22.845470235836398
34	24.779573230274323	24.637385459851604	27.77440810668767	22.808633203186403
35	25.093005568425582	24.20812288325995	27.986697385364582	22.712174162949882
36	29.420086662294914	24.103962997835822	25.57688857273964	20.899061767129616
37	25.244878937950244	27.258372088961437	26.379840616321665	21.11690835676666
38	26.473124050295255	27.310690202143217	24.757185206120663	21.45900054144087
39	24.28838633155552	26.773774573238214	26.05465853499756	22.8831805602087
40	25.333240182216286	24.461695043354965	26.786873949072803	23.418190825355943
41	22.350631389915225	23.856821440423246	27.94509694332019	25.847450226341333
42	24.47352417668438	25.17755608517611	25.869123739085836	24.479795999053668
43	23.861981800600507	24.393419508701957	27.397463643277796	24.34713504741974
44	24.01298187840286	25.563312855965613	27.683823938037573	22.739881327593956
45	22.616509024279097	26.72534657772852	26.905562233149837	23.752582164842543
46	24.11777688507957	25.581175641194598	27.55552944501517	22.745518028710656
47	23.574589433805283	24.864282527338	28.1805681477165	23.38055989114022
48	24.264410504270398	23.87436666502594	28.428344826381668	23.432878004322
49	23.783941276688985	23.44748579313148	28.25710025865313	24.511472671526402
50	22.874765203611936	26.18033315285304	27.75845068521644	23.18645095831858
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	450.0
1	898.5
2	1347.0
3	6180.5
4	11014.0
5	7537.5
6	4061.0
7	4295.0
8	4529.0
9	4735.0
10	4941.0
11	5010.5
12	5080.0
13	4989.0
14	4898.0
15	4756.5
16	4615.0
17	4661.5
18	4708.0
19	4841.0
20	4974.0
21	5198.0
22	5422.0
23	5855.5
24	6289.0
25	7428.5
26	8568.0
27	10126.5
28	11685.0
29	13559.5
30	15434.0
31	17660.5
32	19887.0
33	22992.0
34	26097.0
35	29561.0
36	33025.0
37	37222.5
38	41420.0
39	48564.0
40	55708.0
41	63216.0
42	70724.0
43	75949.0
44	81174.0
45	84912.0
46	88650.0
47	92829.5
48	97009.0
49	100570.0
50	104131.0
51	99702.0
52	95273.0
53	86911.0
54	78549.0
55	73421.5
56	68294.0
57	66009.0
58	63724.0
59	60814.5
60	57905.0
61	54395.5
62	50886.0
63	45696.5
64	40507.0
65	35216.5
66	29926.0
67	25726.0
68	21526.0
69	18282.5
70	15039.0
71	12257.0
72	9475.0
73	7551.0
74	5627.0
75	4531.0
76	3435.0
77	2675.5
78	1916.0
79	1458.0
80	1000.0
81	703.5
82	407.0
83	275.5
84	144.0
85	104.0
86	64.0
87	54.5
88	45.0
89	27.0
90	9.0
91	7.0
92	5.0
93	5.5
94	6.0
95	3.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1259602.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.62577707641115
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.40230790949607	20.153207718820926
2	6.561858326783572	3.7567658733899765
3	3.2911592021196805	2.826359689285715
4	2.2772496913508937	2.607521680477472
5	1.8620858820607822	2.6651827678502187
6	1.5471004250306872	2.6572171129049638
7	1.2918017926799705	2.5885181100915595
8	1.1316882211989914	2.591636379203408
9	1.0402397482587178	2.67999040257085
>10	10.506300048541386	54.52228046237031
>50	0.06928933506170443	1.2599878918206509
>100	0.018084737251965348	1.0086983773838851
>500	0.0	0.0
>1k	8.346801656665176E-4	0.6826335338301155
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3604	0.2861221242900535	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3322	0.2637341001363923	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1645	0.1305968075630239	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03644008186712946	0.0
2	0.0	0.0	0.0	0.11678292031927545	0.0
3	0.0	0.0	0.0	0.18783711045234924	0.0
4	0.0	0.0	0.0	0.30803380750427517	0.0
5	0.0	0.0	0.0	0.47689667053561363	0.0
6	0.0	0.0	0.0	0.8751970860636932	0.0
7	0.0	0.0	0.0	1.0688296779458908	0.0
8	0.0	0.0	0.0	1.661635977078474	0.0
9	0.0	0.0	0.0	2.2345153469111674	0.0
10	0.0	0.0	0.0	3.0175404611932977	0.0
11	0.0	0.0	0.0	3.519762591675783	0.0
12	0.0	0.0	0.0	3.966094051930689	0.0
13	0.0	0.0	0.0	4.204582082276783	0.0
14	0.0	0.0	0.0	4.320332930560606	0.0
15	0.0	0.0	0.0	4.429018054909408	0.0
16	0.0	0.0	0.0	4.594467141208096	0.0
17	0.0	0.0	0.0	4.769125485669282	0.0
18	0.0	0.0	0.0	5.028810687820439	0.0
19	0.0	0.0	0.0	5.153532623797041	0.0
20	0.0	0.0	0.0	5.3017540461193295	0.0
21	0.0	0.0	0.0	5.452992294391403	0.0
22	0.0	0.0	0.0	5.609232122527592	0.0
23	7.93901565732668E-5	0.0	0.0	5.790082899201494	0.0
24	7.93901565732668E-5	0.0	0.0	5.92996835508359	0.0
25	7.93901565732668E-5	0.0	0.0	6.055484192625925	0.0
26	7.93901565732668E-5	0.0	0.0	6.172822844041213	0.0
27	7.93901565732668E-5	0.0	0.0	6.314454883367921	0.0
28	7.93901565732668E-5	0.0	0.0	6.442431815764027	0.0
29	7.93901565732668E-5	0.0	0.0	6.574140085519077	0.0
30	7.93901565732668E-5	0.0	0.0	6.75380000984438	0.0
31	7.93901565732668E-5	0.0	0.0	6.8866197417914545	0.0
32	7.93901565732668E-5	0.0	0.0	7.01896313279909	0.0
33	7.93901565732668E-5	0.0	0.0	7.157102005236575	0.0
34	7.93901565732668E-5	0.0	0.0	7.298892824876429	0.0
35	1.587803131465336E-4	0.0	0.0	7.451798266436541	0.0
36	1.587803131465336E-4	0.0	0.0	7.5916837223186375	0.0
37	1.587803131465336E-4	0.0	0.0	7.730616496321854	0.0
38	1.587803131465336E-4	0.0	0.0	7.89408082870621	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	25	4.443083E-5	44.0	1
TATTCCG	20	7.8567694E-4	44.0	1
TATCGAT	20	7.8567694E-4	44.0	13
CGTTTTT	3880	0.0	40.76804	1
GAGCGAT	1140	0.0	40.333332	7
AGCGATC	165	0.0	40.0	8
CGTTCGA	45	2.3512257E-8	39.111115	14
CGTAAGA	85	0.0	38.82353	2
AGCGATA	215	0.0	37.860466	8
TTAGACG	70	0.0	37.714287	1
GTAACGA	35	7.290764E-6	37.714287	2
CTATCTC	430	0.0	37.348835	6
ACGAGTA	65	1.0913936E-11	37.23077	5
TACGTTC	30	1.301166E-4	36.666668	39
TAGACGA	145	0.0	36.413795	2
GTTTTTA	2185	0.0	36.347824	2
CGCGACA	25	0.0023528298	35.2	16
TCGATCC	25	0.0023528298	35.2	34
TATTGCG	75	1.8189894E-12	35.2	1
GACCGAT	855	0.0	34.994152	9
>>END_MODULE